| Project/Area Number |
12671998
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
KAMIOKA Hiroshi OKAYAMA UNIVERSITY, DENTAL HOSPITAL, ASSISTANT PROFESSOR, 歯学部・附属病院, 講師 (80253219)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Manabu OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE AND DENTISTRY, ASSISTANT, 大学院・医歯学総合研究科, 助手 (40252978)
YAMAMOTO Teruko (TAKANO Teruko) OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE AND DENTISTRY, PROFESSOR, 大学院・医歯学総合研究科, 教授 (00127250)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | mechanical stress / osteocytes / osteoblasts / cytoskeleton / connective tissue growth factor / fluid shear stress / connective tissue growth factor |
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| Research Abstract |
Morphological changes of cytoskeleton in osteoblasts and osteocytes were investigated after subjection to fluid shear stress. It was observed that osteoblastic cell line MC3T3-E1 react to fluid shear stress and changes its cytoskeleton (especially, actin) after application of fluid shear stress of 12 dynes/cm2 for 1 hour. Hence, other cytoskeletal protein, such as vimentin and tubulin did not - change their morphology after adaptation of fluid shear stress. When the cells were microinjected with fluoresncence-labeled vinculin and subjected to fluid shear stress, it was confirmed that the assembly of vinculin was performed within 10 minutes after application of fluid shear stress. So, it was suggested that morphological changes after fluid shear stress happens very rapidly. However, when osteocytes were subjected to fluid shear stress, the cytoskeletal changes was not observed. Based on these results, the cytoskeleton in osteocytes was very stable comparing to osteoblasts. Next, we focused on the stability of osteocyte morphology and observed the three dimensional distribution of osteocyte processes in bone using confocal microscopy and differential interference contrast microscopy.
Furthermore, we found that connective tissue growth factor is a candidate of marker of cytoskeletal changes in bone cells.
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