| Project/Area Number |
12672008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
MAKI Kenshi Kyushu Dental College, Associate Professor, 歯学部, 助教授 (60209400)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Tomoo Kyushu Dental College, Professor, 歯学部, 教授 (30073688)
KIMURA Mitsutaka Kyushu Dental College, Professor, 歯学部, 教授 (70047801)
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| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | matrix vesicles / Alkaline phosphatase / Lactate dehydrogenase / protease / Matrix Vesicles / Lactate dehydrogenase / Alkaline phosphatase |
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| Research Abstract |
The dentin was removed from bovine tooth germs, followed by the separation of the intracellular matrix vesicles fraction after collagenase treatment.
Alkaline phosphatase activity, protease activity, and lactate dehydrogenase activity were assayed for the marker enzyme of matrix vesicles. Matrix vesicles containing alkaline phosphatase, metalloprotease, and lactate dehydrogenase were found in the dentin. We also found matrix vesicle-like vesicles with a high density. Those also contained alkaline phosphatase and lactate dehydrogenase, but not metalloprotease.
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