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Study of the CSF-1 gene effects causing absence of the third molars in mice

Research Project

Project/Area Number 12672020
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionTsurumi University (2001)
Nihon University (2000)

Principal Investigator

ASADA Yoshinobu  Tsurumi Univ., Sch Dent Med, Professor, 歯学部, 教授 (20184145)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
KeywordsEL mice / missing teeth / molecular genetic / linkage analysis / tooth anomaly / 欠如歯 / SSLPマーカー / 遺伝的交配実験
Research Abstract

To identify the candidate chromosome and to detect the region that included causative gene(s) causing absence of the third molars in EL mice, all chromosomes were examined by the individual genotyping of the F2 (EL x MSM) mice. The gene causing absence of the third molars in EL mice was mapped on the region from D3Mit141 to D3Mit290 (x 2 =22.8, p<0.0001). Gene such as Lef1, Egf and CSF-1 that have been shown to be associated with tooth development, are located in this region. To determine whether the congenital absence of the third molars in EL mice occurs due to failure of initiation or subsequent arrest in development, I analyzed H & E stained serial sagittal sections of normal and EL mice. Compared to normal mice, third molars development in EL mice progressed from early cap to cap stage from P3 to P5, but did not progress beyond the cap stage and the developing tooth gradually regressed to complete elimination. I examined whether the absence of the third molars in EL mice occurred due to lack of Lef1 expression. Lef1 expression in EL mice was not different from that observed in normal mice. From these data, it was concluded that Lef1 is not a likely candidate gene involved in the congenital absence of the third molars in EL mice. On the other hand, the candidate chromosomal analysis was also performed on genetic crosses using two strains of mice, EL and DDY. The highest scores were found at D13Mit78 and D13Mit35 markers (x2 =11.8, p<0.01). Based on these results, there was a possibility that a candidate gene causing absence of the third molars might be existed on mouse chromosome 3.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 中村 均: "マウス第3臼歯欠如の成因に対する遺伝学的研究"小児歯科学雑誌. 38・3. 502-508 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshinobu Asada: "Absence of the third molars in strain EL mice"PEDIATRIC DENTAL JOURNAL. 10・1. 19-22 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshinobu Asada: "Detection of the flanking MIT markers for the op genen on mouse chromosome 3"PEDIATRIC DENTAL JOURNAL. 10・1. 29-32 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Reiko Nomura: "Detection of informative SSLP markers for searching a causative gene(s) of absence of the third molars in EL mice"PEDIATRIC DENTAL JOURNAL. 10・1. 75-79 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 朝田 芳信: "DNAマーカー多型検出に対する非放射性SSCP法の有用性"日大口腔科学. 27・1. 1-9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshinobu Asada: "Analysis of Msx1 gene structure in EL mice with absence of the third molar"PEDIATRIC DENTAL JOURNAL. 11・1. 43-48 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazuyuki Saito: "Mapping of a Gene Causing Mouse Hypodontia to Chromosome 13"JAPANESE JOURNAL OF ORAL BIOLOGY. 43・6. 690-699 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hitoshi Nakamura: "Study of the genetically effects causing the absence of the third molars in mice"The Japanese Journal of Rediatric Dentistry. 38(3). 502-508 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshinobu Asada: "Absence of the third molars in strain EL mice"Pediatric Dental Journal. 10(1). 19-22 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshinobu Asada: "Detection of the flanking MIT markers for the op gene on mouse chromosome 3"Pediatric Dental Journal. 10(1). 29-32 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Reiko Nomura: "Detection of informative SSLP markers for searching a causative gene(s) of absence of the third molars in EL mice"Pediatric Dental Journal. 10(1). 75-79 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshinobu Asada: "Utility of a non-radioactive SSCP method for detection of polymorphisms in the DNA markers"Nihon Univ. J. Oral Sci.. 27(1). 1-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshinobu Asada: "Analysis of Msx1 gene structure in EL mice with absence of the third molar"Pediatric Dental Journal. 11(1). 43-48 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazuyuki Saito: "Mapping of a gene causing mouse hypodontia to chromosome 13"Japanese Journal of Oral Biology. 690-699 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 朝田 芳信: "DNAマーカー多型検出に対する非放射性SSCP法の有用性"日大口腔科学. 27・1. 1-9 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshinobu Asada: "Analysisi of Msxl gene structure in El mice with absence of the third molar"PEDIATRIC DENTAL JOURNAL. 11・1. 43-48 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kazumi Saito: "Mapping of a Gene Causing Mouse Hypodontia to Chromosome 13"JAPANESE JOUNAL OF ORAL BIOLOGY. 43・6. 690-699 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 朝田芳信,中村均,前田隆秀: "マウス第3臼歯欠如の成因に対する遺伝学的研究"小児歯科学雑誌. 38・3. 502-508 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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