| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
Periodontitis is a general term for disease categories, including juvenile periodontitis (JP), rapidly progressive periodontitis (RPP), and adult periodontitis (AP), which may or may not share a common etiology and pathogenesis. These disease categories are characterized by difference in progression of tissue destruction and differences in age group susceptibility, but not to our knowledge, by differences in cytokine responses of inflammatory cells. Therefore, the purpose of this study is to investigate the difference of the levels of gingival crevicular Interleukin-1 (IL-1) molecules and the clinical status ot patients with different categories of periodontitis. IL-1 alfa, IL-1 beta, IL-1 receptor antagonist (ra) and the total IL-1/IL-1 ra ratio (IL-1 activity index ; IL-1AI) were measured in 353 gingival crevicular fluid (GCF) samples from 45 non-inflamed gingiva sites in 9 healthy subjects and 215 diseased sites in 50 patients with AP and 93 diseased sites in 18 patients with RPP. IL-1 alfa, IL-1 beta and IL-1 ra were measured by specific non-crossreactive enzyme linked immunosorbent assay (ELISA). The probing depth, gingival index and alveolar bone loss of each site was recorded at the time of GCF sampling. The total amount of IL-1 beta in RPP GCF was higher than AP GCF, however, the total amount of IL-1 alfa was approximately same levels in both AP and RPP GCF. The RPP IL-1AI was significantly higher than AP IL-1AI (p<0.05). The levels of IL-1 molecules in culture supernatant fluids of peripheral blood lymphocyte (PBL), which were cocultured with bacterial lipopolysaccharide, were also compared between AP and RPP. RPP PBL induced more IL-1 beta than AP PBL also. These data suggest that RPP cytokine responses of inflammatory cells are higher than AP and this phenomenon may relate to rapid and sever penodontal tissue destruction in RPP.
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