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The role and mechanism of activation of TRP channel in mast cells

Research Project

Project/Area Number 12672094
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionNagoya City University

Principal Investigator

HIRASHIMA Haohide  Nagoya City University, Graduate School of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (10192296)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsmast cell / TRP channel / calcium channel / exocytosis / allergy / calcium store / IP_3受容体
Research Abstract

Allergic responses are triggered by the exocytotic release of inflammatory mediators such as histamine from mast cells. Although calcium influx from extra-cellular medium is indispensable responsible for this influx is still unknown. Recently, we found that TRP (transient receptor potential) channel, which is activated b the depletion of calcium store, is expressed in mast cells. Thus, we investigated the role of TRP and its activation mechanism in this study.
1. Intracellular Ca dynamics in RBL-2H3 cells with low activity of TRP.
To examine the role of TRP in RBL-2H3 cells, we prepared two types of cells in which the activity of TRP is very low. One is the knock-down cell line in which the expression level of TRP1 is suppressed b anti-sense technique. The other is the dominant negative cell line in which the function of TRP1 is suppressed by the expression of truncated TRP1. In both cell lines, no significant difference on the calcium dynamics was observed. Therefore, it is suggested that TRP1 is not involved in the calcium influx, although TRP1 s expressed in mast cells.
2. The effects of hapten on the intracellular Ca dynamics.
Mast cells are activated by the cross-linking of IgE receptors by multi-valent antigen, and intracellular calcium increase. It is known that addition of mono-valent hapten causes dissociation of IgE receptors and decrease in calcium concentration. To investigate the activation mechanism of calciium influx, we examined the effects of hapten on thapsigargin-induced calcium increase. Hapten did not decrease the calcium increase evoked by thapsigargin. These data suggest that the activation mechanism of calcium channel is different between antigen and thapsigargin stimulation, although both stimulation deplete calcium store.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Tadahide Furuno: "Nuclear shuttling of MAP kinase (ERK2) was dynamically controlled by MEK after antigen stimulation in RBL-2H3 cells"J. Immunol.. 166. 4416-4421 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasushi Satoh: "β-amyloid peptides inhibit acetylcholme release from cholinergic presynaptic nerve endings isolated from the electric"Neuroscience Lett.. 302. 97-100 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tetsuhi Amano: "Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells"J. Biochem.. 129. 739-744 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Seiji Hasegawa: "Microtubule involvements in the intracellular dynamics for gene transfection mediated by cationic liposomes"Gene Therapy. 8. 1669-1673 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshikazu Inoh: "Biosurfactants of MEL-A increase gene transfection mediated by cationic liposomes"Biochem. Biophys. Res. Commun.. 289. 57-61 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tomonobu Yoshimura: "Anchoring and bola cationic amphiphiles for nucleotide delivery"11. 2897-2901 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tadahide Furuno: "Nuclear shuttling of MAP kinase (ERK2) was dynamically controlled by MEK after antigen stimulation in RBL-2H3 cells"J. Immunol. 166. 4416-4421 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasushi Satoh: "β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from the electric ray"Neurosci. Lett. 302. 97-100 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tetsuhi Amano: "Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells"J. Bioche. 129. 739-744 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Seiji Hasegawa: "Microtubule involvements in the intracellular dynamics for gene transfection mediated by cationic liposomes"Gene Theraphy. 8. 1669-1673 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshikazu Inoh: "Biosurfactants of MEL-A increase gene transfection mediated by cationic liposomes"Biochem. Biophys. Res. Commun.. 289. 57-61 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tomonobu Yoshimura: "Anchoring and bola cationic amphiphiles for nucleotide delivery. Effects of orientation and expression of hydrophobic regions"Bioorg. Med. Chem. Lett. 11. 2897-2901 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tadahide Furuno: "Nuclear shuttling of MAP kinase (ERK2) was dynamically controlled by MEK after antigen stimulation in RBL-2H3 cells"J. Immunol.. 166. 4416-4421 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasushi Satoh: "β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from the electric ray"Neuroscience Lett.. 302. 97-100 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tetsuhi Amano: "Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells"J. Biochem.. 129巻. 739-744 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seiji Hasegawa: "Microtubule involvements in the intracellular dynamics for gene transfection mediated by cationic liposomes"Gene Therapy. 8巻. 1669-1673 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshikazu Inoh: "Biosurfactants of MEL-A increase gene transfection mediated by cationic liposomes"Biochem. Biophys. Res. Commun.. 289巻. 57-61 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomonobu Yoshimura: "Anchoring and bola cationic amphiphiles for nucleotide delivery"Bioorg. Med. Chem. Lett.. 11巻. 2897-2901 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirashima,N.: "Intracelluar dynamics of a high affinity NGF receptor TrkA in PC12 cell"Biol.Pharm.Bull.. 23巻. 1097-1099 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomoko Fujiwara: "Gene transfection activities of novel amphiphilic cholestane-polyamine conjugates."Biochim.Biophys.Acta. 1468巻. 396-402 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Dong-Seok Lee: "Rapid transbilayer phospholipid redistribution associated with neurotransmitters from cholinergic nerve"Neuroscience Lett.. 291巻. 21-24 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tadahide Furuno: "Nuclear shuttling of MAP kinase (ERK2) was dynamically controlled by MEK after antigen stimulation in RBL-2H3 cells"J.Immunol.. 166巻(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yasushi Satoh: "β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from the electric ray"Neuroscience Lett.. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tetsuhi Amano: "Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells"J.Biochem.. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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