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Research of gene promoter in Mycobacterium tuberculosis using Fixed-RNA polymerase holoenzymes with the developed resin.

Research Project

Project/Area Number 12672181
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionDaiichi College of Pharmaceutical Sciences

Principal Investigator

ARAMAKI Hironori  Daiichi College of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Lecturer, 薬学部, 講師 (20221054)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsMycobacterium tuberculosis / Genome / Promoter / RNA polymerase / Fixed enzyme / Bioinformatics / バイオインフォーマティクス
Research Abstract

Recombinant RNA polymarase (RNAP) holoenzymes containing different sigma factors, SigA, SigB, SigC, SigD, SigE, SigG, SigH, SigI, respectively, in Mycobacterluin tuberculosis were purified using Escherichia coil expression system. We prepared eight kinds of fixed RNAP holoenzymes with the developed resin. Also, genome library was constructed with PCR using four primers designed from the appearance frequency in M. tuberculosis genome. From this genome library, we selected the DNA fragments, which have high affinity for the fixed RNAP holoenzymes and were diffused with transcription for one cycle.
As a result, SigA type promoters had the consensus sequence TTGACA<16/l7bp>TATAAT as same as E. coil sigma7O promoter consensus sequence. SigH type promoters were conserved 35 regions, comparing with ECF type promoter consensus sequence CCGGAACTT<16/17bp>TCTgA. However, SigE type promoters had no similarity with ECF type promoter, the consensus sequence was predicted GG(aa/gc) (C/A)K18bp>(c/g)GTT(g/c).

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Fujita, M.: "In vitro transcription system using reconstituted RNA polymerase (Es70, E sH, E sE and E sS ) of Pseudomonas aeruginosa"FEMS Microbiol.Lett.. 183. 253-257 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohdo, S.: "Basis for dosing lime-dependent changes in the antiviral activity of interferon-alpha in mice"J.Pharmacol.Exp.Ther.. 294. 488-493 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawashima, T.: "Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium"Proc.Natl.Acad.Sci.USA. 97. 14257-14262 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wang, D.S.: "Effect of dosing schedule on pharmacokinetics of alpha interferon and anti-alpha interferon neutralizing antibody in mice"Antimicrob.Agents.Chemother.. 45. 176-180 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohdo, S.: "Changing the dosing schedule minimizes the disruptive effects of interferon on clock function"Nat.Med.. 7. 356-360 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aramaki, H.: "Transcription analysis of rpoH in Pseudomonas putida"FEMS Microbiol.Lett.. 205. 165-169 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujita, M: "In vitro transcription system using reconstituted RNA polymeitase (Eo70, EoH, EoE and Eos) ofPseudomonas aeruginosa."FEMS Microbiol. Lett.,. 183. 253-257 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohdo, S.: "Basis for dosing time-dependent changes in the antiviral activity of interferon-alpha in mice"J. Pharmacol Exp. Ther.. 294. 488-493 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawashima, T.: "Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasm volcanium."Proc. Ncttl. Acad Sci. USA. 97. 14257-14262 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wang, D.S.: "Effect of dosing schedule on pharmacokinetics of alpha interferon and anti-alpha interferon neutralizing antibody in mice."Antimicrob. Agents. Chemother. 45. 176-180 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohdo, S.: "Changing the dosing schedule minimizes the disruptive effects of interferon on clock function."Nat. Med. 7. 356-360 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aramaki, H: "Transcription analysis ofrpoff m Pseuddmonas putida."FEMS Microbiol. Lett.,. 205. 165-169 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Aramaki: "Transcription analysis of rpoH in Pseudomonas putida"FEMS Microbiology Letters. 205・2. 165-169 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Ohdo: "Changing the dosing schedule minimizes the disruptive effects of interferon on clock function"Nature Medicine. 7・3. 356-360 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Fujita: "In vitro transcription system using reconstituted RNA polymerase (Esigma(70),Esigma(H),Esigma(E) and Esigma (S)) of Pseudomonas aeruginosa."FEMS Microbiol.Lett.. 183・2. 253-257 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Kawashima: "Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium."Proc.Natl.Acad.Sci.USA. 97・26. 14257-14262 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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