Generation of ES cells that stably its translocation to mouse chromosome

• Project/Area Number: 12672200
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human genetics
• Research Institution: Tottori University
• Principal Investigator: OSHIMURA Mitsuo Department of Molecular and Cell Genetics, Faculty of Medicine, Tottori University, Professor, 医学部, 教授 (20111619)
• Co-Investigator(Kenkyū-buntansha): KATOH Motonobu Department of Molecular and Cell Genetics, Faculty of Medicine, Tottori University, Assistant, 医学部, 助手 (00273904)
• Project Period (FY): 2000 – 2001
• Project Status: Completed (Fiscal Year 2001)
• Budget Amount: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: Chromosome 10 / TC mouse / Chromosome stability / Translocation / DT40 / Targeting / Cre-LoxP

Research Abstract

To investigate the gene dosage effects of an extra copy of human chromosome on various phenotypes and the regulatory mechanism, we translocated to the distal region of mChr. 10 by a combination of Cre/loxP mediated chromosome translocation and telomere directed chromosome truncation in embryonic stem (ES) cells and homologous recombination proficient DT40 cells. We are, for example, producing chimeric mice that maintain the DCR form the ES cells. These mice should allow stable maintenance in various tissue and stable germline transmission of the DCR (Down syndrome Critical Region). Furthermore, these ES cells will allow mapping and identification of the genes responsible for various phenotype.

Research Products

• [Publications] Shinohara, T., et al.: "Stability of human chromosome fragments in cultured cells and in mice"Chromosome Res.. 8. 713-725 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shinohara, T., et al.: "Mice containing a human chromosome 21 model behavioral impairment and cardiac anomalies of Down syndrome"Hum. Mol. Genet.. 10. 1163-1175 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kazuki, Y., et al.: "Germline transmission of a transferred human chromosome 21 fragment in transchromosomal mice"J. Hum. Genet.. 46. 600-603 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shinohara, T., Tomizuka, K. Takehara, S., Yamauchi, K., Katoh, M., Ohguma, A., Ishida, I. And Oshimura, M.: "Stability of transferred human chromosome fragments in cultured cells and in mice"Chromosome Res.. 8. 713-725 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shinohara, T., Tomizuka, K., Miyabara, S., Takehara, S., Kazuki, Y., Inoue, J., Katoh, M., Nakane, H., Iino, A., Ohguma, A., Ikegami, S., Inokuchi, K., Ishida, I., Reeves, R.H. and Oshimura, M.: "Mice containing a human chromosome 21 model behavioral impairment and cardiac anomalies of Down syndrome"Hum. Mol. Genet.. 10. 1163-1175 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kazuki, Y., Shinohara, T., Tomizuka, K., Katoh, M., Ohguma, A., Ishida, I. and Oshimura, M.: "Germline transmission of a transferred human chromosome 21 fragment in transchromosomal mice"J. of Hum. Genet.. 46. 600-603 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shinohara, T., et al.: "Mice containing a human chromosome 21 model behavioral impairment and cardiac anomalies of Down syndrome"Hum.Mol.Genet.. 10. 1163-1175 (2001)
• [Publications] Kazuki, Ye., et al.: "Germline transmission of a transferred human chromosome 21 fragment in transchromosomal mice"J.Hum.Genet.. 46. 600-603 (2001)
• [Publications] Shinohara,T., et al.: "Stability of human chromosome fragments in cultured cells and in mice"Chrom.Res.. 8. 713-725 (2000)
• [Publications] Shinohara T., et al.: "Mice containing a human chrmosome 21 model behavioral impairment and cardiac anomalies of Down syndrome"Hum.Mol.Genet.. (in press).