| Project/Area Number |
12672245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kochi Medical School |
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Principal Investigator |
OKUMIYA Toshika Kochi Medical School, Faculty of Medicine, Research Associate, 医学部, 助手 (50284435)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEMOTO Yoshiaki Osaka City University Hospital, Assistant Professor, 医学部・附属病院, 講師 (00254409)
SAIBARA Toshiji Kochi Medical School, Faculty of Medicine, Assistant Professor, 医学部・附属病院, 講師 (60145125)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | erythrocyte creatine / erythropoiesis / liver cirrhosis / splenomegaly / hypersplenism / cardiac valve / intravascular hemolysis / mechanical valve prosthesis / erythropoietin / renal anemia / hemodialysis |
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| Research Abstract |
Erythrocyte creatine is a sensitive marker of erythrocyte age, and can be used to detect slight and continuous hemolysis. Excessive blood cell destruction caused by increased spleen function is important evidence of hypersplenism. We evaluated the usefulness of erythrocyte creatine as a marker of excessive erythrocyte destruction in patients with liver cirrhosis. The patients with splenomegaly showed significantly higher erythrocyte creatine than those without splenomegaly and healthy controls, but there was no significant difference in erythrocyte creatine between healthy controls and those without splenomegaly. Fourteen of the 15 patients with abnormally high erythrocyte creatine had splenomegaly. There were no significant differences in reticulocyte count between healthy controls and the patients with and without splenomegaly. Erythrocyte creatine showed good correlation with spleen size. Thus, erythrocyte creatine can be used for estimating hypersplenism in patients with liver cirr
… More
hosis.
Cardiac vale replacement with mechanical prosthesis is associated with mild intravascular hemolysis. To estimate the intravascular hemolysis due to cardiac valve dysfunction, we analyzed erythrocyte creatine in 32 patients with prosthetic mitral and/or aortic valve, and the data were compared with cardiac valve flow dynamics. No significant differences in erythrocyte creatine and other hemolytic markers including serum lactic dehydrogenase, haptoglobin, indirect bilirubin and reticulocyte counts were observed between the patients in the presence and absence of regurgitations. Although erythrocyte creatine, serum lactic dehydrogenase and haptoglobin in the patients with prosthetic valve showed significant differences as compared to those in healthy controls, total peak transvalvular flow velocities (peak transmitral flow velocity + peak transaortic flow velocity) had significant correlation with only erythrocyte creatine. These results suggest that the intravascular hemolysis is attributed chiefly to transvalvular flow velocity rather than regurgitation in patients with mechanical valve prosthesis, and hence erythrocyte creatine is a possible marker for assessment of cardiac valve dysfunction. Less
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