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STUDIES ON REGULATION OF STEROID HORMONE SYNTHESIS AND METABOLISM IN FETO-PLACENTAL UNIT

Research Project

Project/Area Number 12672258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKobe Pharmaceutical University

Principal Investigator

KOBAYASHI Yoshiharu  KOBE PHARMACEUTICAL UNIVERSITY, FACULTY OF PHARMACY, ASSOCIATE PROFESSOR, 薬学部, 助教授 (20133647)

Co-Investigator(Kenkyū-buntansha) FUJINAMI Aya  Kobe Pharmaceutical University, Faculty of Pharmacy, Assistant, 薬学部, 助手 (30299086)
TAGAWA Noriko  Kobe Pharmaceutical University, Faculty of Pharmacy, Assistant, 薬学部, 助手 (00206905)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords16-dehydropregnenolone / EIA / mother-placenta / pregnenolone / steroid metabolism / 17-hydroxylation / 16-hydroxylation / GC / MS / エストリオール
Research Abstract

We had identified 16-dehydropregnenolone sulfate (16-DHPS) as a novel steroid in serum from neonate. We also revealed that this steroid is synthesized according to the following process : Pregnenolone sulfate (PregS)→16-hydroxy-PregS (16-OH-PregS) →16-DHPS. However, the physiological significance of 16-DHPS is unclear. The aim of this study is to investigate whether this metabolic pathway regulate the known steroid pathway, PregS→17-hydroxy-PregS (17-OH-PregS)→dehydroepiandrosterone sulfate (DHEAS)→16-OH-DHEAS, because these pathways use common substrate, PregS. Firstly, we developed methods for the measurement of these steroids : PregS, 16-OH-PregS, 16-DHPS, 17-OH-PregS, DHEAS and 16-OH-DHEAS by GC/MS. Methods for the measurement of their unconjugated steroids by EIA were also established (Biol. Pharm. Bull. 24,867 (2001). Using these methods, serum concentrations of these steroids were determined in the samples from pregnant women. Key step of the above two pathways is 17-hydroxylation and 16-hydroxylation of Preg(S). 17- and 16-hydroxylase activities were obtained by the ratio of product/substrate concentration that is 17-OH-Preg(S)/Preg(S) ratio and 16-OH-Preg(S)/Preg(S) ratio, respectively. As a result, the 17-hydroxylase activities were decreased according to the course of pregnancy. Inversely, the 16-hydroxylase activities were increased at term compared with those at the first trimester of pregnancy. There were no significant changes of the product/substrate ratios for the other steroids in the above pathway. Present data suggest that 17-hydroxylation of Preg(S) is to be regulated by 16-hydroxylation of Preg(S).

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Noriko Tagawa, Aya Fujunami, Nobuyuki Amino, Yoshiharu Kobayashi et al.: "Heterogeneous enzyme immunoassay for serum androstenediol"Clin.Chim.Acta. 296. 193-201 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Noriko Tagawa, Kayoko Saiki, Yoshiharu Kobayashi: "Development of an enzyme immunoassay for serum 16-dehydropregnenolone"Biol.Pharm.Bull.. 24. 867-871 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Noriko Tagawa, Aya Fujinami, Yoshiharu Kobayashi et al.: "Heterogeneous enzyme immunoassay for serum androstenediol"Clin. Chim. Acta. Vol.296 No.1, 2. 193-201 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Noriko Tagawa, Kayoko Saiki, Yoshiharu Kobayashi: "Development of an enzyme immunoassay for serum 1 6-dehydropregnenolone"Biol. Pharm. Bull.. Vol.24 No.8. 867-871 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Noriko Tagawa, Aya Fujunami, Nobuyuki Amino, Yoshiharu Kobayashi et al.: "Heterogeneous enzyme immunoassay for serum androstenediol"Clin. Chim. Acta. 296巻1,2号. 193-201 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Noriko Tagawa, Kayoko Saiki, Yoshiharu Kobayashi: "Development of an enzyme immunoassay for serum 16-dehydropregnenolone"Biol. Pharm. Bull.. 24巻8号. 867-871 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Noriko Tagawa,Satoshi Kusuda and Yoshiharu Kobayashi: "16-Dehydropregnenolone 3-sulfate, Its source and metabolism in the feto-placental unit"Biol.Pharm.Bull.. 22巻12号. 1262-1265 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Noriko Tagawa,Junko Tamanaka,Aya Fujinami,Yoshiharu Kobayashi et al: "Serum dehydroepiandrosterone, dehydroepiandrosterone sulfate, and pregnenolone sulfate concentrations in patients with hyperthyroidism and hypothyroidism"Clin.Chem.. 46巻4号. 523-528 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Noriko Tagawa,Junko Tamanaka,Aya Fujinami,Yoshiharu Kobayashi et al: "Heterologous enzyme immunoassay for serum androstenediol"Clin.Chim.Acta. 296巻. 193-201 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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