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Establishment of Cell Strains Deficient in Genes Related to Mutation Induction, and Analysis of Mutation Mechanisms Using the Cell Strains.

Research Project

Project/Area Number 12680542
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionOsaka Rrefecture University

Principal Investigator

YAGI Takashi  Research Institute for Advanced Science and Technology, Osaka Prefecture University, Professor, 先端科学研究所, 教授 (80182301)

Co-Investigator(Kenkyū-buntansha) TAKEDA Shun-ichi  Graduate School of Medicine, Kyoto University, Professor, 医学研究科, 教授 (60188191)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsMutation / DNA Damage / DNA Polymerase / DNA Adduct / Environmental Mutagen
Research Abstract

Recently, several kinds of DNA polymerases have been found in Eschericnia coil and mammalian cells. The first purpose of this study is to elucidate roles of these DNA polymerases on mutation induction and DNA damage avoidance. The second purpose of this study is to investigate the types of base changes arisen from specific base damage by these DNA polymerases.
We cloned DNA polymerase ζ(Rev3) cDNA from chicken DT40 cells, and established hprpt (+/-) heterozygous cell, lines by the somatic recombination method to utilize mutation assay. We also established a DT40 cell line deficient in xeroderma pigmentosum A (XPA) gene, and comfirmed UV hypersensitivity of the cell line.
We constructed the plasmid containing one 3-nitrobenzanthrone (NBA) adduct on a guanine base of the plasmid and rendered the plasmid to replicate in E. coil. We found that an adenine is inserted at the opposite site of the guanine having a NBA adduct. We found that bydroquinone, a metabolite of benzene, produces reactive oxygene species in the presence of NADH and copper ions, and causes G : C →A : T transition mutations in mammalian cells.
These results clarified a part of the pathway leading to base changes from DNA damage, and provided the advance toward the clarification of complete mutation mechanisms.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 八木孝司: "DT40細胞株で致死的変異を導入したコンディショナルミュータントをつくる"実験医学. 18. 67-73 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Otoshi Eriko: "Respective roles of cyclobutane pyrimidine dimers, (6-4)photoproducts and minor photoproducts in UV mutagenesis of repair deficient xeroderma pigmentosum A cells"Cancer Research. 60. 1729-1735 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawanishi Masanobu: "Postlabelling analysis of DNA adducts formed in human hepatoma cells treated with 3-nitrobenzanthrone"Mutation Research. 470. 133-139 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中山 亜紀: "ベンゼン代謝生成物による染色体異常誘発リスクの評価"放射線生物研究. 36. 120-135 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakayama Aki: "Comparison of the mutation induced by p-benzoquinone, a benzene metabolite, in human and mouse cells"Mutation Research. 470. 147-153 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 八木孝司: "クローズアップ実験法総集編"葛西文明. 213 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Yagi: "To Establish Conditionally Lethal Mutants of DT40 Cell strains."Experimental Medicine. 18. 67-73 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aki Nakayama: "Comparison of the mutations induced by p-benzoquinone, a benzene metabolite, in human and mouse cells."Mutation Research. 470 (2). 147-153 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masanobu Kawanishi: "Postlabelling analysis of DNA adducts formed in human hepatoma cells treated with 3-nitrobenzanthrone."Mutation Research. 470 (2). 133-139 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Eriko Otoshi: "Respective roles of cyclobutane pyrimidine dimers, (6-4) photoproducts and minor photoproducts in UV mutagenesis of repair deficient xeroderma pigmentosum A cells."Cancer Research. 60. 1729-1735 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aki Nakayama: "Risk Evaluation for Chromosome Aberrations Induced by Benzene Metabolites."Radial. Biol. Res. Commun.. 36 (2). 120-135 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Yagi: "To Establish Conditionally Lethal Mutants of DT40 Cell strains."In: "Experimental Protocol Close Up". Experimental Medicine Special Issue.. 64-72 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 八木孝司: "DT40細胞株で致死的変異を導入したコンディショナルミュータントをつくる"実験医学. 18. 67-73 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Otoshi Eriko: "Respective roles of cyclobutane pyrimidine dimers, (6-4) photoproducts and minor photoproducts in UV mutagenesis of repair deficient xeroderma pigmentosum cell"Cancer Research. 60. 1729-1735 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawanishi Masanobu: "Postlabelling analysis of DNA adducts formed in human hepatoma cells treated with 3-nitrobenzanthrone"Mutation Research. 470. 133-139 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakayama Aki: "Comparison of the mutations induced by p-benzoquinone, a benzene metabolite, in human and mouse cells"Mutation Research. 470. 147-153 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中山亜紀: "ベンゼン代謝生成物による染色体異常誘発リスクの評価"放射線生物研究. 36巻2号. 120-135 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 八木孝司: "クローズアップ実験法1999-2001"葛西文明((株)羊土社)(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 八木孝司: "DT40細胞株で致死的変異を導入したコンディショナルミュータントをつくる"実験医学. 18. 67-73 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] OTOSHI Eriko: "Respective roles of cyclobutane pyrimidine dimers,(6-4) photoproducts and minor photoproducts in UV mutagenesis of repair deficient xeroderma pigmentose A cells."Cancer Research. 60. 1729-1735 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] KAWANISHI Masanobu: "Postlabelling analysis of DNA adducts formed in human hepatoma cells treated with 3-nitrobenzanthrone."Mutation Research. 470. 133-139 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] NAKAYAMA Aki: "Comparison of the mutations induced by p-benzoquinone, a benzene metabolite, in human and mouse cells."Mutation Research. 470. 147-153 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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