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Analysis on various toxic actions of organotins including tri-n-butyltin

Research Project

Project/Area Number 12680544
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionThe University of Tokushima

Principal Investigator

OYAMA Yasuo  The University of Tokushima, Faculty & Integrated Arts and Sciences, Professor, 総合科学部, 教授 (80214229)

Co-Investigator(Kenkyū-buntansha) KANEMARU Kaori  The University of Tokushima, Faculty & Integrated Arts and Sciences, Lecturer, 総合科学部, 講師 (00243676)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordstri-n-butyltin / organotin / apoptosis / NMDA / environmental pollution / cytotoxicity / neurotoxicity / immunotoxicity / トリプチル錫 / 脳神経細胞 / 胸腺細胞 / 細胞死 / アポトーシス / 環境影響評価 / トリフェニル錫
Research Abstract

(A) As tri-n-butyltin (TBT), one of environmental pollutants, is accumulated in wild animals, concern regarding the toxicity of TBT in both wildlife and humans is increasing. TBT has been reported to increase intracellular Ca2+ concentration in several types of cells. In order to examine how Ca2+ is involved in TBT-induced cell death, the effect of TBT on rat thymocytes has been compared with that of A23187, a calcium ionophore, under various concentrations of external Ca2+ using a flow cytometer and fluorescent probes. Although both TBT and A23187 were toxic to cells under normal Ca2+ condition, under external Ca2+-free condition the cytotoxic action of TBT was potentiated without changing the threshold concentration while that of A23187 was completely abolished. A23187 attenuated the TBT-induced descent in cell viability under normal Ca2+ concentration despite intracellular Ca2+ concentration was increased. As external Ca2+ concentration increased, the TBT-induced increase in number … More of dead cells gradually decreased whereas the number of cells in an early stage of apoptosis increased. Results suggest that Ca2+ has contradictory actions on the process of TBT-induced cell death in rat thymocytes.
(B) The effects of tri-n-butyltin (TBT), an environmental pollutant, on membrane currents elicited by N-methyl-D-asparlate (NMDA) were examined in the neurons acutely dissociated from the dorsal motor nucleus of vagus of rats, using a nystatin-perforated patch recording mode under the voltage-clamp conditions, in order to predict a neurotoxic action of TBT on humans and wild animals. TBT at an environmentally-relevant concentration (l00 nM) greatly increased the amplitude of NMDA-induced outward current, but not that of NMDA-induced inward current. The reversal potential of the NMDA-induced outward current was close to the theoretical K+ equilibrium potential of -82 mV. The NMDA-induced outward current augmented by TBT was markedly inhibited by 5 mM tetraethylammonium chloride and 300 nM charybdotoxin. The outward current was abolished by removal of extracellular Ca2+, suggesting that the outward current was due to the activation of Ca2+-activated K+ channels by Ca2+ passing through NMDA channels. The NMDA-induced outward current was completely blocked by Cs+-internal solution. Under this condition, TBT had no effect on the NMDA-induced inward current. The present results suggest that TBT potentiates Ca2+-activated K+ current induced by NMDA without augmenting the NMDA-induced inward current. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Kanemoto Y. et al.: "Modification of NMDA responses by tri-n-butyltin in rat brain neurons"The British Journal of Pharmacology. 136. 35-46 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Oyama Y. et al.: "Effects of A23187 and CaCl2 on tri-n-butyltin-induced cell death in rat thymocytes"Environmental Toxicology and Pharmacology. 13. 29-36 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Arata T. et al.: "Cytotoxic effects of triphenylbismuth on rat thymocytes : Comparison with bismuth chloride and triphenyltin chloride"Environmental Toxicology. 17. 472-477 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kishimoto K. et al.: "Nanomolar concentrations of tri-n-butyltin facilitate aminobutylic acidergic synaptic transmission in rat hypothalamic neuronss"Journal of Pharmacology and Experimental Therapeutics. 299. 171-177 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai K. et al.: "Tri-n-butyltin delays the cell death induced by hydrogen peroxide in rat thymocytes"Environmental Toxicology and Pharmacology. 10. 95-101 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okada Y. et al.: "Tri-n-butyltin-induced decrease in cellular level of glutathione in rat thymocytes : a flow cytometric study using 5-CMF-DA"Toxicology Letters. 117. 128-128 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Oyama Y., Arata T., Chikahisa L., Umebayashi C. and Hayashi H.: "Effects of A23187 and CaCl2 on tri-n-butyltin-induced cell death in rat thymocytes."Environmental Toxicology and Pharmacology. 13. 29-36 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanemoto Y., Ishibashi H., Matsuo S., Oyama Y. and Akaike N.: "Modification of NMDA responses by tri-n-butyltin in rat brain neurons."The British Journal of Pharmacology. 136. 35-46 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Arata T., Oyama Y., Satoh M., Hayashi H., Okano Y.: "Cytotoxic effects of triphenylbismuth on rat thymocytes : Comaprison with bismuth chloride and triphenyltin chloride"Environmental Toxicology. 17. 472-477 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kishimoto K., Matsuo S., Kanemoto Y., Ishibashi H., Oyama Y. and Akaike N.: "Nanomolar concentrations of tributyltin facilitate gamma-aminobutylic acidergic synaptic transmission rat hypothalamic neurons"Journal of Pharmacology and Experimental Therapeutics. 299. 171-177 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai K., Oyama Y., Okada Y., Akaike N., Nakata M., Chikahisa L.: "Tri-n-butyltin delays the cell death induced by hydrogen peroxide in rat thymocytes"Environmental Toxicology and Pharmacology. 10. 95-101 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okada Y., Oyama Y., Chikahisa L., Kanemaru K., Sakai H., Noda K., Satoh M.: "Tri-n-butyltin-induced decrease in cellular level of glutathione in rat thymocytes : a flow cytometric study using 5-CMFDA"Toxicology Letters. 117. 123-128 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanemoto Y.et al.: "Modification of NMDA responses by tri-n-butyltin in rat brain neurons"The British Journal of Pharmacology. 136. 35-46 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oyama Y.et al.: "Effects of A23187 and CaCl2 on tri-n-butyltin-induced cell death in rat thymocytes"Environmental Toxicology and Pharmacology. 13. 29-36 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kei Sakai: "Tri-n-butyltin delays the cell death induced by hydrogen peroxide in rat thymocytes"Environmental Toxicology and Pharmacology. 10. 95-101 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kiyonori Kishimoto: "Nanomolar concentrations of tri-n-butyltin facilitate γ-aminobutyric acidergic synaptic transmission in rat hypothalamus neurons"Journal of Pharmacology and Experimental Therapeutics. 299. 171-177 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasuo Oyama: "Protective action of tri-n-butyltin on the cells suffering from oxidative stress : Its implication in bioaccumulation"Natural Science Research. 14. 9-20 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshihiko Okada: "Cytotoxic action of tri-n-butyltin hydride, a nucleophilic organotin, on rat thymocytes : a flow cytometric study"Natural Science Research. 14. 21-34 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Okada,Y. et al.: "Tri-n-butyltin-induced change in cellular level of glutathione in rat thymocytes : a flow cytometric study"Toxiology Letters. 117. 123-128 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Okada,Y. et al.: "Cytotoxic action of tri-n-butyltin hydride, a nucleophilic triorganotin, on rat thymocytes : a flow cytometric study"Natural Sciences Research. 14. 21-34 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Oyama,Y. et al.: "Protective action of tri-n-butyltin on the cells suffering from oxidative stress : Its implication in bioaccumulation"Natural Sciences Research. 14. 9-19 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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