| Project/Area Number |
12680558
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | Kitasato University (2001)
National Institute for Environmental Studies (2000) |
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Principal Investigator |
KUNIMOTO Manabu Kitasato University, School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (20142101)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Neurotoxicity / Development / Neurite extension / Biomarker / Gene expression / In vitro / 環境化学物質 / 神経発生毒性 / 培養神経細胞 / 器官培養系 / マーカー蛋白質 / スクリーニング / 画像解析 |
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| Research Abstract |
Development of a simple assay system that can evaluate the neurotoxicity of environmental chemicals especially on the developmental process of nervous system has been attempted. As a primary screening system, evaluation of degree of neurite extension in human neuroblastoma NB-1 cell by the computer image analysis, was used together with immunochemical determination of axonal marker proteins. Many chemicals (more than 10% of tested chemicals) turned out to affect the neurite extension in the NB-1 cell during the primary screening. Among 250 chemicals tested phthalate and cadmium remarkably promoted the neurite extension, while the neurite extension was remarkably suppressed by methylmercury, a cause of Minamata disease. In order to analyze the action mechanism at the molecular level, effects of cadmium and methylmercury on the gene expression in NB-1 cells was analyzed using CDNA macroarray and differential display methods, and several genes were identified to be affects by them.
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