| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
In order to investigate the receptor molecules for H.pylori in human, this study involved
1)Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaecharide-conjugated neoglycoconjugates. "These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to" "ceramide, sphingomyelin, or polyacrylamides conjugated with β-galactose, lactose, 3'-" "sialyllactose or 3'-sulfo-β-galactose. Furthermore, neoglycolipids or BSA-derivatives" with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with α-hydroxyl fatty acid was much stronger than GalCer with the non-hydroxyl fatty acid.
2) When direct adhesion of H. pylori to immobilized human fluids, all of the clinical "isolated strains strongly adhered to immobilized human gastric juice. On the other hand, " 2 of 5 strains did not adhered to Leb-antigen and one strain did not adhered to sulfatide. The adhesion of H. pylori to human gastric juice remarkably reduced when gastric juice "immobilized on the plate were oxidized by periodate, but that was not affected by the" "treatment with sialidase, indicating that the receptors in gastric juice are non-sialylated" "glycoconjugates. In addition, Leb-oligosaccharides, sulfatide, and sulfated" oligosaccharides did not inhibit the adhesion to gastric juice.
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