| Project/Area Number |
12680685
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUZUKI Emiko The University of Tokyo, The Institute of Medical Science, Research Associate, 医科学研究所, 助手 (20173891)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Drosophila / diacylglycerol kinase / visual mutant / retial degeneration / signal transduciton / phototransduciton / immunocyrochemistry / electron microscopy / 免疫電子顕微鏡法 / 視細胞 / 網膜変成 / 光情報交換 / システインリッチドメイン / 免疫組織化学 |
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| Research Abstract |
The final goal of this research project is to elucidate the mechanism for the functional expression of the diacylglycerol kinase (RDGA/DAGK2) that is a key enzyme of the phototransduction in Drosophila photoreceptors. Our previous studies by immnoelectron microscopy of photoreceptor cells revealed that RDGA protein localizes to the subcellular region at the bases of photoreceptive microvilli, subrhabdomeric region in which a specialized network of smooth endoplasmic reticulum resides. In the present study, we studied the domain functions of RDGA protein by the expression studies on the modified RDGA proteins. Our results demonstrated that the cysteine-rich domains (CRDs) of the RDGA protein are essential for the localization and enzyme activity of the protein. On the other hand, the ankyrin repeats at the C-terminus was unnecessary for the primary function of RDGA protein, suggesting that they have other functions. This remains to be uncovered by the future studies.
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