| Project/Area Number |
12680689
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
NAKASHIMA Shigeru Gifu University, School of Medicine, Department of Biochemistry, Professor, 医学部, 教授 (60188935)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HISAMOTO Naoki Nagoya University, Graduate School of Science, Division of Biological Science, Research Associate, 院理, 助手 (80283456)
BANNO Yoshiko Gifu University, School of Medicine, Department of Biochemistry, Associate Professor, 医学部, 助教授 (50116852)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | Caenorhabditis elegans / phospholipase D / pld-1 gene / cDNA / phospholipase D superfamily / cell-specific expression / gene knockout / GFP / SL-1 |
|---|
| Research Abstract |
The gene encoding phospholipase D (PLD) of Caenorhabditis elegans (pld-1) was isolated. The isolated cDNA encodes a 1,427 amino acid protein which contains four conserved regions defined by the primary structures of bacterial, plant, yeast and mammalian PLDs. Furthermore, HKD motifs (HxKxxxxD) in regions II and IV, which are critical for PLD activity, are completely conserved. The corresponding genomic sequence was covered by the cosmid clone C04G6. Comparison of the sequences between C04G6 and the cloned cDNA revealed that PLD gene has 18 exons and that the transcription unit is 7.8 kb. An extensive screening of the complete C. elegans genome by the BLAST algorithm has revealed that PLD-1 is the sole member of the PLD family which contains four conserved regions including two catalytic HKD motifs. The protein expressed in COS-7 cells catalyzed transphosphatidylation reaction to generate phosphatidylbutanol in the presence of 0.3 % butanol. It also released [^3H]choline from [^3H]phosphatidylcholine, indicating that expressed protein exhibited PLD activity. This PLD activity was dependent on phosphatidylinositol 4,5-bisphosphate and weakly stimulated by ADP-ribosylation factor. However, it was strongly inhibited by oleic acid. These properties are similar to those of mammalian PLD2. To determine where pld-1 is expressed, we constructed a translational fusion between pld-1 and the green fluorescent protein (GFP) to generate pld-1::GFP. This construct contains about 4.7 kb pld-1 promoter regions in addition to first 668 amino acids. PLD-1 was expressed in pharyngeal muscles and most of neurons. Weak expression was also observed in epithelial cells, body-wall muscles, vulval muscles and excretory canal.
|
