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Role of transcription factors and a differentiation inhibiting factor which concern differentiation of a common primordiam of adrenal cortex and gonad.

Research Project

Project/Area Number 12680720
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNara Medical University

Principal Investigator

HATANO Osamu  NARA MEDICAL UNIVERSITY, Medicine, Assistant Professor, 医学部, 講師 (40164850)

Co-Investigator(Kenkyū-buntansha) HAGA Satomi  NARA MEDICAL UNIVERSITY, Medicine, Assistant Professor, 医学部, 講師 (20192263)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsAd4BP / SF-1 / Pref-1 / WT1 / Emx2 / Adrenal Cortex / Gonad / Development / Organogenesis / 副腎皮質 / 生殖腺 / 器官形成 / As4BP / WT-1 / 胚発生
Research Abstract

Our previous study on expression profiles of Ad4BP/SF-1 in rat embryos demonstrated that steroid hormone-producing cells of adrenal cortex and gonads are derived from a common precursor named "adreno-genital primordium". The analysis of differentiation process of the primordium revealed that it consists of four steps. The aim of this project is to seek molecules which control differentiation of the adreno-genita primordium and examine the role of the factors. It was suggested that Pref-1/ZOG, WT1 , Emx2 and Ad4BP/SF-1 are the factors that control the differentiation of the adreno-genital primordium. Pref-1/ZOG was already expressed in the adreno-genital primordium and continued to be expressed only in the adrenocortical primordium but not in the gonadal primordium (=indifferent gonad). WT1 was expressed in the gonadal primordium, but not in the adrenocortical primordium. Analyses of Pref-1 gene promoter with co-transfection assays have shown that WT1 down-regulates the transcription of Pref-1 gene. On the other hands, Emx2-deficient mice fails to form gonads, but was not affected to form adrenal glands, suggesting that Emx2 acts in a different manner in the process of differentiation of the adreno-genital primordium. Emx2 also functions in the differentiation of brain. Using Emx2- and/or Emx1-deficient mice and anti-Emx2 antibody, we examined the role of Emx2 in the differentiation of brain and also in regeneration process of neural precursor cells after ischemic brain injury.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Hatano, O., et al.: "Genesis of the adrenal cortex and gonads from their common primordium"Frontier Science Series Universal Academy Press Inc. (Tokyo). 29. 197-200 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tekamori, H., et al.: "Characterization of proximal element in the rat Pref-1 (ZOG) gene promoter"Frontier Science Series Universal Academy Press Inc. (Tokyo). 29. 209-212 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harada, N., et al.: "Histological lesions in aromatase-deficient mice"Frontier Science Series Universal Academy Press Inc. (Tokyo). 29. 133-136 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suda, Y., et al.: "Emx2 directs the development of diencephalon in cooperation with Otx2"Development. 128. 2433-2450 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takemori, H., et al.: "Characterization of proximal element in the rat preadipocyte factor-1 (Pref-1) gene promoter"Eur.J.Biochem.. 268. 205-217 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakatomi, H., et al.: "Regeneration of hippocampal pyramidal neurons after ischemic brain injury by recruitment of endogeneous neural progenitors"Cell. 110. 429-441 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 秦野 修: "基礎生化学実験法 第3巻 タンパク質 I.検出・構造解析法(第5章「細胞内・組織上での検出」)"東京化学同人. 282 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suda Y, Hossain ZM, Kobayashi C. Hatano O. Yoshida M, Matsuo I and Aizawa S.: "Emx2 directs the development of diencephalon in cooperation with Otx2"Development. 128(13). 2433-2450 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takemori H, Doi J, Katoh Y, Helder SK, Lin XZ, Horike N, Hatano O and Okamoto M.: "Characterization of a proximal element in the rat preadipocyte factor-1 (Pref-1 ) gene promoter"Eur. J. Biochem.. 268(2). 205-217 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Haga S, Nakayama M, Tatsumi K, Maeda M, Imai S, Umesako S, Yamamoto H, Hilgers J & Sarker S.: "Overexpression of the p53 gene product in canine mammary tumors"Oncology Reports. 8. 1215-1219 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakatomi H, Kuriu T, Okabe S, Yamamoto S, Hatano O. Kawahara N, Tamura A, Kirino T, Nakafuku M.: "Regeneration of hippocampal pyramidal neurons after ischemic brain injury by recruitment of endogeneous neural progenitors"Cell. 110. 429-441 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakao Y, Haga S, Umesako S and Imai S.: "Expression of p53 tumor suppressor gene product in human colorectal cancer"J. Analytical Bio-Science. 25. 315 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hatano O. Takemori H, Okamoto M and Tohno Y.: "Genesis of the adrenal cortex and gonads from their common primordium, Frontier Science series No. 29 "Molecular Steroidogenesis""Universal Academy Press Inc.. 197-200 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takemori H, Doi J, Kato Y, Hatano O and Okamoto M.: "Characterization of proximal element in the rat Pref1 (ZOG) gene promoter, Frontier Science series No. 29, "Molecular Steroidogenesis""Universal Academy Press Inc.. 209-212 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harada N, Honda S, Hirasawa G, Hatano O and Sasano H.: "Histological lesions in aromatase-deficient mice, Frontier Science series No. 29, "Molecular Steroidogenesis""Universal Academy Press Inc.. 133-136 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hatano O., Ishimura-Y eds.: "Chapter 5, Methods of protein detection in cells and tissues, in "Basic Methods of Biochemistry ".Vol. 3, Protein I. Methods of Detection and Structural Analysis"Tokyo Kagaku Dojin Inc.. 32-38 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto H, Hashimoto K, Haga S. Nagano-Tatsumi K, Maeda M, Sakumura R and Hamed MS: "Chapter 5, Nuclear Spatial Organization, in "Electron Microscopy in Medicine and Biology""Science Publishers, Inc.. 59-66 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Maeda M. Haga S. Nagano-Tatsumi K, Yamamoto H, Fukui A, Nakamura T, Tamai S, Ogata S, Iwata H and Ikeda Y.: "Chapter 9, Mechanism of Graft Healing. in "Electron Microscopy in Medicine and Biology""Science Publishers, Inc.. 99-107 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakatomi H, et al.: "Regeneration of hippocampal pyramidal neurons after ischemic brain injury by recruitment of endogenous neural progenitors"Cell. 110. 429-441 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakao T, et al.: "Expression of p53 tumor suppressor gene product in human colorectal cancer tissue"J. Analytical Bioscience. 25. 315-319 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 芳賀敏実 他: "柔整用語辞典"海馬書房(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suda Y et al.: "Emx2 directs the development of diencephalon in cooperation with Otx2"Development. 128(13). 2433-2450 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takemori H et al.: "Characterization of a proximal element in the rat preadipocyte factor-1 (Pref-1) gene promoter"Eur. J. Biochem. 268(2). 205-217 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 秦野 修: "第3巻 タンパク質I.検出・構造解析法 第5章「細胞内・組織上での検出」"基礎生化学実験法(東京化学同人、日本生化学会編). 第3巻. 32-38 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takemori-H, et al.: "Characterization of a proximal element in the rat preadipocyte factor-1 (Pref-1) gene promoter."Eur.J.Biochem.. 268. 205-217 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hatano-O, et al.: "Genesis of the adrenal cortex and gonads from their common primordium."Frontier Science Series : Molecular Steroidogenesis.(Universal Academy Press Inc.). 29. 197-200 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takemori-H, et al.: "Characterization of proximal element in the rat Pref-1 (ZOG) gene promoter."Frontier Science Series : Molecular Steroidogenesis.(Universal Academy Press Inc.). 29. 209-212 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Harada-N, et al.: "Histological legions in aromatase-deficient mice."Frontier Science Series : Molecular Steroidogenesis.(Universal Academy Press Inc.). 29. 133-136 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 秦野修: "基礎生化学実験法 第3巻 第5章 細胞内・組織上での検出"東京化学同人. 7 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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