| Project/Area Number |
12680725
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Okazaki National Research Institutes |
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Principal Investigator |
SHIMONO Akihiko Okazaki National Research Institutes Research Associate, 基礎生物学研究所, 助手 (10321605)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Lim1 / Head formation / Visceral endoderm / Cell movement / 下流遺伝子 / オーガナイザー / 転写因子 |
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| Research Abstract |
Stoma regulates regional visceral endoderm movements during mouse embryogenesis In mouse pregastrula stage embryos, visceral endoderm (VE) migrates from a distal to anterior position relative to the epiblast. This anterior visceral endoderm (AVE) is then displaced away from the epiblast apparently by the definitive endoderm to become associated with the extra-embryonic ectoderm. A differential screen between the anterior mesendoderm of single wild type and Liml mutant gastrula stage embryos yielded a CDNA clone (designated Stoma) that was expressed in the AVE ofgastrula stage wild type embryos in a pattern that was similar to Liml but absent or significantly reduced in Liml-nuli embryos. In this study, the role ofStoma gene in VE movements has been investigated. Stoma expression is initially detected in the AVE and subsequently in the VE associated with the extra embryonic ectoderm. Most Stoma mutant mice die soon after gastrulation with distinct furrows ofVE located at the anterior junction of the embryonic and extra-embryonic regions. Mutant analysis suggests that VE accumulation in these furrows is caused by defects in cell migration into proximal extra embryonic regions, although distal to anterior movements associated with the epiblast, definitive endoderm formation, and anterior specification of the epiblast appear normal. These data suggest that Stoma is required for VE movements in extra-embryonic regions that are independent of distal to anterior movements in the embryonic portion.
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