| Project/Area Number |
12680734
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Saga Medical School |
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Principal Investigator |
MASUKO Sadahiko Saga Medical School, Dept of Anatomy and Physiology, Professor, 医学部, 教授 (20015100)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Yuzo Saga Medical School, Dept of Anatomy and Physiology, Assistant, 医学部, 助手 (20128143)
LI Ming-zi Saga Medical School, Dept of Anatomy and Physiology, Assistant, 医学部, 助手 (00304882)
KAWANO Hitosi Saga Medical School, Dept of Anatomy and Physiology, Assistant Professor, 医学部, 助教授 (10152985)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Sacral spinal cord / Parasympathetic preganglionic neuron / Visceral afferent nerve / Inter Neuron / Spinal cord descending fiber / Neuropeptide / Vesicular acetylcholine transporter / Vanilloid Reception 1 / Vanilloid Receptor 1(VR1) / 脳幹 / カテコラミン / モノアミン / 中枢性投射線維 / 内臓性求心線維 / シナップス構築 / vesicular acetylcholine transporter |
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| Research Abstract |
To clarify the neural regulation to parasympathetic preganglionic neurons, nerve fibers and terminals distributing around sacral preganglionic neurons of the rat were investigated by using immunohistochemistry combined with axonal racing and nerve transection experiments, and the following results were obtained.
1. About 50-60% of the sacral preganglionic neurons were immunoreactive for NO synthase.
2-1). In the sacral spinal cord, substance P (SP)-, calcitonin gene-related peptide (CGRP)-, vesicular acetylcholine transporter (VAChT)-, and vasoactive intestinal polypeptide (VIP)-immunoreactive fibers extending from Lissauer's tract along the lateral edge of the dorsal horn to the autonomic nucleus were found, and fibers immunoreactive for SP and CGRP formed a dense networks of nerve terminals around the preganglionic cells.
2). Although, both lumbar and sacral preganglionic neurons received enkephalin (ENK)-immunoreactive nerve fibers from the dorsal central gray, ENK fibers in the sacral
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spinal cord also contained neuropeptide Y (NPY) and formed nerve terminals mainly distributing outside of the CGRP/SP nerve networks.
3). Lumbar sympathetic preganglionic neurons received many serotonin (5-HT)- and tyrosine hydroxylase (TH)-immunoreactive descending fibers. In contrast, 5-HT and TH fibers around sacral preganglionic neurons were scarce and. Did not showed selective distribution.
3. These fibers did not disappear after the transection of the L4, L5 spinal cord, suggesting that they are of intrinsic or of peripheral origin.
4. Transection experiments of the spinal nerve revealed that VAChT, VIP, SP and CGRP fibers were of peripheral origin, and VAChT and VIP fibers came from the myenteric neurons in the most distal potion of the rectum.
5. In the pelvic visceral sensory system, the expression of vanilloid reception 1 (VR1) was restricted to the afferents from the ureter and fundus of urinary bladder, the superficial dorsal horn and fibers in the lateral collateral pathway extending along the lateral edge of the dorsal horn into the parasympathetic preganglionic nucleus, suggesting that these VR1-positve afferents were involved in regulation of the sacral preganglionic neurons. Less
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