| Project/Area Number |
12680736
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Nerve anatomy/Neuropathology
|
|---|
| Research Institution | OSAKA CITY UNIVERSITY |
|---|
Principal Investigator |
MAEDA Mitsuyo Osaka City University, Graduate School of medicine, Lecturer, 大学院・医学研究科, 講師 (40122080)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANAKA Akemi Osaka City University, Graduate School of medicine, Assistant Professor, 大学院・医学研究科, 助教授 (30145776)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | brain tumor / gene therapy / microglia / adenovirus / GFAP / Cre-loxP |
|---|
| Research Abstract |
We examined the intraarterial gene delivery system into tumor brain using isolated microglia as a carrier. At new born mouse, microglia isolated from a mixed glial culture drawn from neonatal mice were retained the capability to migrate into the brain parenchyma after intra-arterial injection. Although a few microglia migrated into the brain parenchyma in adult mouse, rat and also Mongolian gerbil, almost of them were retained in the brain capillary. So we stopped the use of this delivery system, and decided the use of the adenovirus- mediated gene-transfer system using Cre-loxP system. This system was included astrocyte specific GFAP promoter and chymidine kinase gene, and showed the inhibitory effect of proliferation of cultured astrocyte. Now experiment of gene therapy using C6 glioma is on going.
|
