Novel Factor Oxidized Galectin-1 Advances Functional Recovery after Peripheral Nerve Injury

• Project/Area Number: 12680758
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Waseda University (2001); Yokohama City University (2000)
• Principal Investigator: HORIE Hidenori Waseda University, Institute for Biomedical Science, Professor, 先端バイオ研究所, 教授 (80046135)
• Project Period (FY): 2000 – 2001
• Project Status: Completed (Fiscal Year 2001)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,700,000); Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000); Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: Oxidized galectin-1 / regeneration / peripheral nerve / functional recovery / injury / transection / 酸化型がレクチンー1 / 軸索再生 / 機能再建 / 末梢神経損傷 / 指間距離 / 坐骨神経 / 末梢神経再生 / マクロファージ / シュワン細胞 / 促進因子

Research Abstract

We have revealed that oxidized galectin-1 promotes axonal regeneration from transected-nerve sites in an in vitro DRG explant model as well as in vivo peripheral nerve axotomy models. The purpose of this project is to clarify whether oxidized galectin-1 advances restoration of nerve functions after peripheral nerve injury.
The sciatic nerve of an adult rat was transected and then the distal nerve was frozen after sutured into proximal site with four epineurial stitches. Peripheral delivery of oxidized galectin-1 or control solvent to the surgical site was performed with the osmotic pump. The functional recovery was evaluated by measuring the degree of toe spread of the hind paw. The recovery curves indicated that oxidized galectin-1 administration clearly advanced functional recovery (p<0.05 compared with control PBS group by ANOVA). The histological study showed that oxidized galectin-1 increased numbers of regenerating myelinated axons in a sciatic nerve after axotomy. There was the tendency that diameters of the myelinated axons were large in oxidized galectin-1 administrating group. These results indicate that administration of oxidized galectin-1 to the nerve injury sites is effective to the rapid restoration of the nerve function.
We have performed the purpose of this project during its period and are now preparing to publish a paper of this work.

Research Products

• [Publications] Shuto T, Horie H, et al.: "IL-6 upregulates CNTF mRNA expression and enhances neurite regeneration"NeuroReport. 12. 1081-1085 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kato T., Kurita K et al.: "Galectin-1 is a component of neurofilamentous lesions in sporadic and familial amyotrophic lateral sclerosis"Biochem.Biophys.Res.Commun. 282. 166-172 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hirono M, Sugiyama T et al.: "Phospholipase Cβ4 and protein kinase Ca and/or protein kinase CβI are involved in the induction of long term depression in cerebellar purking"J.Boil.Chem.. 276. 45236-45242 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takano M, Horie H et al.: "Brain-derived neurotrophic factor enhances neurite regeneration from retinal ganglion cells in aged human retina in vitro"Exp.Eye Res.. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Saito H, Sango K. et al.: "Trachea enhances neurite regeneration from adult rat nodose ganglia in vitro"Life Sci.. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishioka T, Sakumi K et al.: "fosB gene products trigger cell proliferation and morphological alteration with an increased expression of a novel processed form of galectin-1 in the rat 3Y1 embryo cell line"J.Biochem.. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishioka T, Sakumi K, Miura T, Tahara K, Horie H, Kadoya T, and Nakabeppu Y.: "fosB gene products trigger cell proliferation and morphlogical alteration with an increased expression of a novel processed form of galectin-1 in the rat 3Y1 embryo cell line"J Biochem. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oshitari T, Dezawa M, Okada S, Takano M, Negishi H, Horie H, Sawada H, Tokuhisa T, Adachi-Usami E.: "Suppression of c-fos mediates inhibition of cell death and increases neurite regeneration in retinal ganglion cells"Invest. Ophthalmol. Vis. Sci.. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takano M, Horie H, Iijima Y, Dezawa M, Sawada H, Ishikawa Y.: "Brain-derived neurotrophic factor enhances neurite regeneration from retinal ganglion cells in aged human retina in vitro"Exp Eye Res. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hirono M, Sugiyama T, Kishimoto Y, Sakai I, Miyazawa T, Kishio M, Inoue H, Nakao K, Ikeda M, Kawahara S, Kirino Y, Katsuki M, Horie H, Ishikawa Y, Yoshioka T.: "Phospholipase Cβ4 and protein kinase Cα and/or protein kinase CβI are involved in the induction of long-term depression in cerebellar purkinje cells"J. Biol Chem. 276. 45236-45242 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kato T, Kurita K, Seino T, Kadoya T, Horie H, Wada M, Kawanami T, Daimon M, Hirano A.: "Galectin-1 is a component of neurofilamentous lesions in sporadic and familial amyotrophic lateral sclerosis"Biochem Biophes Res Commun. 282. 166-172 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shuto T, Horie H, Hikawa N, Sango K, Tokashiki A, Murata H, Yamamoto I, and Ishikawa Y.: "IL-6 upregulates CNTF mRNA expression and enhances neurite regeneration"NeuroReport. 12. 1081-1085 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Horie, H. and Kadoya, T.: "Identification of oxidized galectin-1 as an initial repair regulatory factor after axotomy in peripheral nerves"Neurosci. Res.. 38. 131-137 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Inagaki, Y., Sohma, Y., Horie, H., Nozawa, R. and Kadoya, T.: "Oxidized galectin-1 promotes axonal regeneration in peripheral nerves but does not possess lectin properties"Eur. J. Biochem.. 267. 2955-2964 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shuto T, Horie H. et al.: "IL-6 unregulated CNTF mRNA expression and enhances neurite reqereration"Neuro Report. 12. 1081-1085 (2001)
• [Publications] Kato T., Kurita K et al.: "Galectin-1 is a component of neurofilamentous lesions in sporadic and familial amyotrophic lateral sclerosis"Biochem. Biophys. Res. Commun. 282. 166-172 (2001)
• [Publications] Hirono M, Sugiyama T. et al.: "phospholipase CB4 and protein Kinase Cx and/or protein Kinase CBI are involed in the induction of long-term depression in cerebellar purkinje cell"J. Biol. Chem.. 276. 45236-45242 (2001)
• [Publications] Takano M, Horie H et al.: "Brain derived neurotrophic factor enhances neurite regeneration from retinal ganglion cells in aged human retina invitro"Exp. Eye Res.. (in press).
• [Publications] Saito H, Sango K. et al.: "Trachea enhances neurite regeneration from adult rat nodose ganglia in vitro"Life Sci.. (in press).
• [Publications] NishioKaT, SakumiK et al.: "fosB gene products trigger cell proliferation and morphological alteration with an increased expression of a novel processed form of galectm-1 in the rat3Y lonlnyo cell"J. Biochem.. (in press).
• [Publications] Inagaki Y,Sohma Y,Harie H,Nozawa R and Kadoya T: "Oxidized Galectin-1 promotes axonal regeneration in peripheral nerves but does not possess lectin properties."Eur.J.Biochem.. 267. 2955-2964 (2000)
• [Publications] Horie,H and Kadoya.T.: "Identification of oxidized galectin-1 as an intial repair regulatory factor after axotomy in peripheral nerves."Neurosci.Res.. 38. 131-137 (2000)
• [Publications] Shuto T,Horie H,Hikawa N,Sango K,Tokashiki A,Murata H,Yamamoto I, and Ishikawa Y.: "IL-6 upregulates CNTF mRNA expression and enhances neurite regeneration."Neuro Report. (in press). (2001)
• [Publications] 堀江秀典: "神経再生を促進する新たな神経活性化因子"神経研究の進歩. 44. 453-462 (2000)
• [Publications] 堀江秀典,門屋利彦,稲垣好昌,相馬良明: "新しいタイプの神経再生促進物質酸化型ガレクチン-1"生化学誌. 72. 1245-1249 (2000)
• [Publications] 堀江秀典: "神経再生と神経栄養因子"特集「神経再生と機能修復」Clinical Neuroscience. 18. 19-22 (2000)