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Investigation on migration mechanisms of glial progenitors: observations of living cells labeled by dual fluorescent molecules

Research Project

Project/Area Number 12680770
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionNiigata University

Principal Investigator

KAKITA Akiyoshi  Brain Research Institute, Niigata University, Associate Professor, 脳研究所, 助教授 (80281012)

Co-Investigator(Kenkyū-buntansha) YAMADA Mitsunori  Brain Research Institute, Niigata University, Associate Professor, 脳研究所, 助教授 (30240039)
TAKAHASHI Hitoshi  Brain Research Institute, Niigata University, Professor, 脳研究所, 教授 (90206839)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordssubventricular zone / non-radial migration / tangential migration / radial fiber / glial progenitor / corpus callosum / retrovirus / fluorescent labeling
Research Abstract

The great majority of glial cells of the mammalian forebrain are generated in the perinatal period from progenitors in the subventricular zone (SVZ). We investigated the migration of progenitors from the neonatal (postnatal day 0, PO) rat forebrain SVZ by labeling them in vivo with a GFP-retrovirus, and monitoring their movements by time-lapse video microscopy in P3 slices. We identified a small number of progenitors that migrated tangentially within the corpus callosum (CC) and crossed the midline. These retained a relatively uniform morphology: the leading process was extended toward the contralateral side, but showed no process branching or turning away from the migratory direction. Net migration requires the elongation of the leading process and nuclear translocation, and the migrating cells in the CC showed both modes. We confirmed the presence of unmyelinated axon bundles within the P3 CC, but failed to detect any radially directed glial processes (vimentin- or GLAST-immunolabeled fibers) spanning through the CC. The destination of the callosal fibers was examined by applying DiI to the right cingulum; the labeled fibers ran throughout the CC and reached the left cingulate and motor areas. The distribution and final fates of the retrovirus-labeled cells were examined in P28 brains. A small proportion of the labeled cells, less than 1%, were found in the contralateral hemisphere, where, as oligodendrocytes and astrocytes, they colonized predominantly the cortex and the underlying white matter of the cingulate and secondary motor areas. The distribution pattern appears to coincide well with the projection direction of the callosal fibers. Thus, glial progenitors migrate across the CC, presumably in conjunction with unmyelinated axons, to colonize the contralateral hemisphere.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 柿田明美: "発生期脳における未分化細胞の移動経路と動態"新潟医学会雑誌. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akiyoshi Kakita: "Expprimentally induced leptomeningeal glioneuronal heterotopia and underlying cortical dysplasia of the lateral limbic area in rats treated transplacentally with methylmercury"Journal of Neuropathology and Experimental Neurology. 60・8. 768-777 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akiyoshi Kakita: "Aprosencephaly : histopathological features of the rudimentary forebrain and retina"Acta Neuropathologica. 102・1. 110-116 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akiyoshi Kakita: "Migration pathways and behavior of glial progenitors in the postnatal forebrain"Human Cell. 14・1. 59-75 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akiyoshi Kakita: "Intrauterine methylmercury intoxication : consequence of the inherent brain lesions and cognitive dysfunction in maturity"Brain Research. 877・2. 322-330 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akiyoshi Kakita: "Distinct pattern of neuronal degeneration in the fetal rat brain induced by consecutive transplacental administration of methylmercury"Brain Research. 859・2. 233-239 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akiyoshi Kakita: "Understanding of Minamata Disease. Methylmercury Poisoning in Minamata and Niigata. Japan."Taidzawa Y and Osame M. 154 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakita A., Wakabayashi K., Su M., Piao Y.-S., Takahashi H.: "Experimentally induced leptomeningeal glioneuronal heterotopia and underlying cortical dysplasia of the lateral limbic area in rats treated transplacentally with methylmercury"Journal of Neuropathology and Experimental Neurology. 60 (8). 768-777 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakita A., Hayashi S., Arakawa M., Takahashi H.: "Aprosencephaly: histopathological features of the rudimentary forebrain and retina"Acta Neuropathologica. 102 (1). 110-116 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakita A.: "Migration pathways and behavior of glial progenitors in the postnatal forebrain"Human Cell. 14 (1). 59-75 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakita A., Wakabayashi K., Su M., Yoneoka Y., Sakamoto M., Ikuta F., Takahashi H.: "Intrauterine methylmercury intoxication: consequence of the inherent brain lesions and cognitive dysfunction in maturity."Brain Research. 877 (2). 322-330 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakita A., Wakabayashi K., Su M., Sakamoto M., Ikuta F., Takahashi H.: "Distinct pattern of neuronal degeneration in the fetal rat brain induced by consecutive transplacental administration of methylmercury"Brain Research. 859 (2). 233-239 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakita A., Wakabayashi, K., Su M., Sakamoto M., Ikuta F., Takahashi H.: "lntrauterine methylmercury intoxication: pattern of neuronal degeneration in the fetal brain and consequence on higher brain function in maturity"Understanding of Minamata Disease. Methylmercury Poisoning in Minamata and Niigata, Japan. 154 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 柿田明美: "発生期脳における未分化細胞の移動経路と動態"新潟医学会雑誌. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Experimentally induced leptomeningeal glioneuronal heterotopia and underlying cortical dysplasia of the lateral limbic area in rats treated transplacentally with methylmercury"Journal of Neuropathology and Experimental Neurology. 60・8. 768-777 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Aprosencephaly : histopathological features of the rudimentary forebrain and retina"Acta Neuropathologica. 102・1. 110-116 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Migration pathways and behavior of glial progenitors in the postnatal forebrain"Human Cell. 14・1. 59-75 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Intrauterine methylmercury intoxication : consequence of the inherent brain lesions and cognitive dysfunction in maturity"Brain Research. 877・2. 322-330 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Distinct pattern of neuronal degeneration in the fetal rat brain induced by consecutive transplacental administration of methylmercury"Brain Research. 859・2. 233-239 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Understanding of Minamata Disease. Methylmercury Poisoning in Minamata and Niigata, Japan. ed. by Takizawa Y and Osame M"Japan Public Health Association. 154 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Migration pathways and behavior of glial progenitors in the postnatal forebrain."Human Cell. 14・1(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Aprosencephaly : histopathological features of the rudimentary forebrain and retina."Acta Neuropathologica. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Intrauterine methylmercury intoxication. Consequence of the inherent brain lesions and cognitive dysfunction in maturity."Brain Research. 877. 322-330 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akiyoshi Kakita: "Distinct pattern of neuronal degeneration in the fetal rat brain induced by consecutive transplacental administration of methylmercury."Brain Research. 859. 233-239 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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