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Construction of congenic strains from epileptic model rat NER

Research Project

Project/Area Number 12680810
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionHokkaido University (2002-2003)
Kyoto University (2000-2001)

Principal Investigator

KITADA Kazuhiro  Hokkaido University, Center for Advanced Science and Technology, Associate professor, 先端科学技術共同研究センター, 助教授 (70263093)

Co-Investigator(Kenkyū-buntansha) SERIKAWA Tadao  Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (30025655)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Keywordsepilepsy / animal model / NER / genetic analysis / congenic strain / 疾患モデル動物 / コンジェニック系統 / 対照系統
Research Abstract

NER is an animal model of epilepsy that exhibit spontaneous tonic-clonic convulsions, beginning with neck and forelimb clonus, wild jumping/running, opisthotonic posturing, and evolving to tonic, then clonic convulsion, followed by postictal flaccidity. Ictal cortical and hippocampal EEGs are characterized by high-voltage spikes followed by diffuse spike-and-wave or polyspike-and-wave complexes. Seizure susceptibility to pentylenetetrazol, tossing, and transcorneal electroshock, but not to tactile, photic, or acoustic stimuli, or to transauricular electroshock. No pathologic changes were demonstrated in the CNS. Spontaneous convulsions are controlled by a major autosomal recessive gene for epilepsy, that are comparable to generalized tonic-clonic seizures in humans
In this project, we have generated two congenic lines of F344.NER-ner1^<NER>/ner1^<NER> and NER.F344-ner1^<F344>/ner1^<F344>. The former is expected to to show GTC, and the latter is expected not to show GTC. In the future, it is considered that strict animal experiment for reseach on epilepsy will be possible using NER, F344 and these 2 lines of congenic strains.

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All 2003 Other

All Journal Article (6 results) Publications (18 results)

  • [Journal Article] Involvement of Ca2+ Channels in abnormal excitability of hippocampal CA3 pyramidal cells in Noda epileptic rats2003

    • Author(s)
      Kiura, Y.ら
    • Journal Title

      J Pharmacol Sci 91(2)

      Pages: 137-144

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Identification and distribution of aspartoacylase in the postnatal rat brain2003

    • Author(s)
      Klugmann, M.ら
    • Journal Title

      Neuroreport 14(14)

      Pages: 1837-1840

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Activation by N-acetyl-L-aspartate of acutely dissociated hippocampal neurons in rats via metabotropic gluamate receptors2003

    • Author(s)
      Yan, H-D.ら
    • Journal Title

      Epilepsia 44(9)

      Pages: 1153-1159

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Identification and distribution of aspartoacylase in the postnatal rat brain.2003

    • Author(s)
      Klugmann, M., Symes, CW., Klaussner, BK., Leichitlein, CB., Serikawa, T., Young, D., During, MJ
    • Journal Title

      Neuroreport. 14(14)

      Pages: 1837-1840

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Activation by N-acetyl-L-aspartate of acutely dissociated hippocampal neurons in rats via metabotropic gluamate receptors.2003

    • Author(s)
      Yan, H-D., Ishihara K., Serikawa, T., Sasa, M.
    • Journal Title

      Epilepsia. 44(9)

      Pages: 1153-1159

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Involvement of Ca^<2+> Channels in abnormal excitability of hippocampal CA3 pyramidal cells in Noda epileptic rats.2003

    • Author(s)
      Kiura, Y., Hanaya, R., Serikawa, T., Kurisu, K., Sakai, N., Sasa, M.
    • Journal Title

      J Pharmacol Sci 91

      Pages: 137-144

    • NAID

      130000073715

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kiura, Y.: "Involvement of Ca2+ Channels in abnormal excitability of hippocampal CA3 pyramidal cells in Noda epileptic rats"J Pharmacol Sci. 91(2). 137-144 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Klugmann, M.: "Identification and distribution of aspartoacylase in the postnatal rat brain"Neuroreport. 14(14). 1837-1840 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yan, H-D.: "Activation by N-acetyl-L-aspartate of acutely dissociated hippocampal neurons in rats via metabotropic gluamate receptors"Epilepsia. 44(9). 1153-1159 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuwamura M: "The myelin vacuolation (mv) rat with a null mutation in the attractin gene"Lab Invest.. 82(10). 1279-1286 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Baslow, M.H.: "The effects of lithium chloride and other substances on levels of brain N-acetyl-L-aspartic acid in canavan disease-like rats"Neurochem Res.. 27(5). 403-406 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yokoi, N.: "Cblb is a major susceptibility gene for rat type 1 diabetes mellitus"Nature Genet.. 31(4). 391-394 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Seki, T: "Adenoviral gene transfer of aspartoacylase into the tremor rat, a genetic model of epilepsy, as a trial of gene therapy for inherited epileptic disorder"Neuroscience Lett.. 328(3). 249-252 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakane, Y: "A novel mutation vf causing abnormal vacuoles in the central nervous system maps on rat chromosome 8"Exp Anim.. 51(2). 149-155 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakane, Y: "Comparative mapping of 31 rat genes"Exp Anim.. 51(1). 109-111 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kuramoto, T. et al.: "Attractin/Mahogany/Zitter plays a critical role in myelination of the central nervous system"Proc Natl Acad Sci USA. 98(2). 559-564 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Gunn, T.M.et al.: "Molecular and Phenotypic Analysis of Attractin Mutant Mice"Genetics. 158(4). 1683-1695 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakane, Y. et al.: "Comparative mapping of 31 rat genes"Exp Anim. 51(1). 109-111 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Baslow.M.et al.: "The effect of lithium chloride and other substances on levels of brain N-acethyl-L-asparatic acid in Canavan disease-like rats"Neurochem. Res.. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Maihara,T.,Noda,A.,Yamazoe,H., et al: "Chromosomal mapping of genes for epilepsy in NER : a rat strain with tonicclonic seizures."Epilepsia. 41(8). 789-790 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kuramoto,T.,Kitada,K.,Inui,T., et al.: "Attractin/Mahogany/Zitter plays a critical role in myelination of the central nervous system."Proc Natl Acad Sci USA. 98(2). 559-564 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitada,K.,Voigt,B.,Kondo,Y.and Serikawa,T.: "An integrated rat genome map based on genetic and cytogenetic data."Exp Anim. 49. 119-126 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitada,K.,Akimitsu,T.,Shigematsu,Y., et al.: "Accumulation of N-acetyl-L-aspartate in the brain of the tremor rat, a mutant exhibiting absence-like seizure and spongiform degeneration in the central nervous system."J.Neurochem. 74. 2512-2519 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yokoi,N.,Shimizu,S.,Ishibashi,K., et al.: "Genetic mapping of the rat mutation creeping and evaluation of its positional candidate gene reelin."Mamm Genome. 11. 111-114 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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