| Project/Area Number |
12680813
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kochi Medical School |
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Principal Investigator |
FURUYA Masato Kochi Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00035437)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Yoshiya Kochi Medical School, Faculty of Medicine, Assistant, 医学部, 助手 (60243846)
TOMINAGA Akira Kochi Medical School, Faculty of Medicine, Professor, 医学部, 教授 (50172193)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | eosinophilic leukocytes / Leishmania protozoa / H_2O_2 / IL-5 / IR-4 / IFN-γ / INF-γ / リーシュマニア |
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| Research Abstract |
The Leishmania protozoa killing mechanisms by eosinophilic leukocytes were investigated in vivo and in vitro experiments using IL-5 transgenic mice (C3H/HeN-TgN (IL-5) Imeg ; IL-5 TG mouse) and BALB/c mice. The following results were obtained.
1. It was observed that growth of lesion site and protozoan proliferation in the infection site of IL-5 TG mice infected with L. amazonensis (L. a.) were significantly controlled compared with those of C3H/HeN mice.
2. Eosinophilic leukocytes separated from IL-5 TG mice showed remarkable killing activity to L. a. protozoa under presence of IL-4 or INF- γ. This killing activity was inhibited under presence of the catalase. From these results, it was thought that the effecter molecule of eosinophils, which participates in L. a. protozoan killing, was H_2O_2.
3. The degranulation of the eosinophilic leukocytes was not participating in the protozoan-killing action of the eosinophilic leukocytes.
4. The protozoan matter and IL-4 were required for the chemotaxis of the eosinophilic leukocytes.
5. The suppression of the growth of lesion and the increase of IL-4 and INF-γ in peripheral blood were observed in BALB/c mice (having a high sensitivity to Leishmania) made to increase the eosinophils by IL-5 inoculation. Furthermore, when LPS treatment was performed to these mice, it also became clear that a depressor effect was reinforced.
6. The functional and morphological difference did not accept between the eosinophilic leukocytes of TG mice and the cells of BALB/c mice.
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