• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of HTLV-I Tax transgenic mice as an animal model for adult T-cell leukemia by using T cell specific gene promoter.

Research Project

Project/Area Number 12680816
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionKumamoto University (2001-2002)
Juntendo University (2000)

Principal Investigator

OHSUGI Takeo  Kumamoto University, Division of Microbiology and Genetics, Center for Animal Resources and Development, associate professor, 動物資源開発研究センター, 助教授 (00211102)

Co-Investigator(Kenkyū-buntansha) 羅 智靖  順天堂大学, 医学部, 助教授 (60230851)
久原 孝俊  順天堂大学, 医学部, 助教授 (70134616)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsHTLV-I / Tax / mice / transgenic mice / Lck
Research Abstract

Human T-cell leukemia virus type I (HTLV-I) is a causative agent that induces adult T-cell leukemia/lymphoma (ATL). To investigate the therapeutic agent for ATL, it is necessary to establish an animal model. The HTLV-I regulatory protein Tax has been postulated to lead to the proliferation and transformation of T-cells in ATL. In this study, we attempted to develop transgenic (Tg) mice expressing HTLV-I Tax preferentially in T-cells under the control of a distal or proximal lck promoter that direct high expression in mature or immature T-cells, respectively. Tumors developed on the liver and lung of proximal-Tax #15, and on the liver, spleen and lung of distal-Tax #6. These tumors were not derived from T-cells. Histological examination of kidneys in proximal-Tax #15 and #16, distal-Tax #6 and #7 showed glomerular nephritis, whereas the nontransgenic mice had no lesion. No transgenic progeny were obtained from these founder mice. Therefore, offspring with Tax gene was obtained using in vivo fertilization technique in proximal-Tax Tg mice, and the change of genetic background of distal-Tax Tg mice from C57BL/6 to BDF1 produced offspring with Tax gene. Pathological and molecular biological analyses of these mice are in progress at our laboratory.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report

Research Products

(3 results)

All Other

All Publications (3 results)

  • [Publications] Ohsugi, T.: "Transmission of human T-cell leukaemia virus type I into adult mice"Laboratory Animals. 34. 439-445 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohsugi T.: "Transmission of human T-cell leukaemia virus typeI into adult mice."Laboratory Animals. 34. 439-445 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohsugi,T: "Transmission of human T-cell leukaemia virus type I into adult mice"Laboratory Animals. 34. 439-445 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi