| Project/Area Number |
12680817
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory animal science
|
|---|
| Research Institution | Azabu University |
|---|
Principal Investigator |
SHINO Masao Azabu University, Animal Science and Biotechnology, Associate Professor, 獣医学部, 助教授 (30063960)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KASHIWAZAKI Naomi Azabu University, Animal Science and Biotechnology, Associate Professor, 獣医学部, 助教授 (90298232)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
|
|---|
| Keywords | Rat / Spermatozoa / Freezing / Equex Stem / Intrauterine Insemination / 凍結保存 |
|---|
| Research Abstract |
The ultimate aim of the present study is to establish a reliable method for cryopreservation of rat spermatozoa, which can be, produced pups derived from frozened/thawed spermatozoa. Based on previous results of this project, the ability of frozen/thawed spermatozoa to generate normal offspring by intrauterine insemination was evaluated. Rat spermatozoa were frozen with various concentrations of glycerol (0, 3 and 6%) and with or without 0.7% Equex Stem (ES) as cryoprotective agents. Females induced pseudo-pregnancies on DAY-0 were inseminated into the oviductal end of both uterine horns (intrauterine insemination) with post-thaw spermatozoa. On DAY-22 the inseminated females underwent Caesarean section to confirm pregnancy and normality of fetuses. Foster mothers who had given birth the day before the Caesareans nursed several offspring derived from cryopreserved spermatozoa. When inseminated with spermatozoa frozen with ES and without glycerol, the rate of pregnancy and the number of obtained fetuses were significantly higher. However, these results of frozen/thawed sperm insemination as well as the rate of development to the blastocyst stage after intrauterine insemination, which was obtained last year, indicated poor results of fresh spermatozoa. We also confirmed the fertility of the rats derived from frozen/thawed spermatozoa by progeny tests. These results indicate that rat frozen/thawed spermatozoa can generate normal offspring by intrauterine insemination.
|
