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Regeneration of immature tissue (fetal liver) functions utilizing a tissue-engineering approach

Research Project

Project/Area Number 12680823
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionUniversity of Tsukuba

Principal Investigator

MIYOSHI Hirotoshi  Institute of Basic Medical Sciences, Department of Biomedical Engineering, University of Tsukuba, Assistant Professor, 基礎医学系, 講師 (70292547)

Co-Investigator(Kenkyū-buntansha) OOKAWA Keiko  Institute of Basic Medical Sciences, Department of Biomedical Engineering, University of Tsukuba, Assistant Professor, 基礎医学系, 講師 (30251052)
OHSHIMA Norio  Institute of Basic Medical Sciences, Department of Biomedical Engineering, University of Tsukuba, Professor, 基礎医学系, 教授 (50015971)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordshepatocyte / artificial liver / tissue engineering / bone marrow / fetus / oncostatin M / three-dimensional culture / hematopoiesis / oncostatin M
Research Abstract

Fetal livers are known to perform hepatic and hematopoietic functions, and contain undifferentiated cells abundantly. In the current study, long-term three-dimensional (3-D) cultures of fetal liver cells (FLCs) as well as bone marrow cells (BMCs) were performed from the tissue engineering viewpoints, and abilities of proliferation and differentiation of these cells were investigated.
As a 3-D scaffold, porous polyvinyl formal (PVF) resin was adopted, and the 3-D cultures as well as conventional monolayer cultures as controls were performed. In the long-term 3-D cultures of murine FLCs for up to 1 month, the FLCs secreted albumin, a representative liver-specific function, more abundantly and stably than those in the monolayer cultures. Moreover, proliferation and albumin secretion of the FLCs were dramatically stimulated by supplementation of oncostatin M, especially in the 3-D cultures.
In the 3-D perfusion cultures of the murine and porcine FLCs, these cells easily lost their viability … More and functions under conditions of ordinary perfusion rate, suggesting that FLCs are quite susceptible to medium flow rates. However, porcine FLCs were rather stable in these perfusion cultures than murine cells, and showed prolonged albumin secretion ability under a low flow rate condition. Therefore, the development of a bioartificial liver using 3-D cultured porcine FLCs is expected to be realistic.
Respecting hematopoietic functions, 3-D cultures of murine BMCs were performed. In the 3-D cultures, high-density cultures of BMCs were achieved, and these cells contained progenitor cells more abundantly than in the monolayer cultures. Moreover, proliferation of the progenitor cells in the 3-D cultures was found to be considerably improved either by coculture of the BMCs with stroma cells or by cultures under a lowered ambient oxygen tension. From these facts, the 3-D cultures of BMCs were considered to be a promising method for effective expansion of the bone marrow immature cells as well. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] 柳 健一, Thein Tun, 三好浩稔: "バイオ人工肝臓ヘの応用を目的とする充填層型リアクタの開発:リアクタの性能改善とスケールアップ"肝臓. 41(3). 226-227 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyoshi H, Ehashi T, Ema H, et al.: "Long-term culuture of fetal liver cells using three-dimentional porous polymer substrate"ASAIO J. 46(4). 397-402 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tun T, Miyoshi H, Ema H, et al.: "New type of matrix support for bone marrow cell culture: in vitro culture and in vivo transplantation experiments"ASAIO J. 46(5). 522-526 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 三好浩稔, 大島宣雄: "人工肝臓:この一年の進歩"人工臓器. 29(5). 602-604 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang TH, Miyoshi H, Ohshima N: "Novel cell immobilization method utilizing centrifugal force to achieve high-density hepatocyte culuture in porous scaffold"J Biomed Mater Res. 55(3). 379-386 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyoshi H, Ehashi T, Ohshima N: "Long-term three-dimentional culuture of fetal liver cells to achieve prolonged metabolic functions"Tissue Engineering for Therapeutic Use. 6(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tun T, Miyoshi H, Aung T, et al.: "Effect of growth factors on ex vivo bone marrow cell expansion using three-dimensional matrix support"Artif Organs. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aung T, MiYoshi H, Tun T, Ohshizma N: "Chondroinduction of mouse mesenchymal stem cells in three-dimensional highly porous matrix scaffolds"J Biomed Mater Res. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yanagi K, Tun T, Miyoshi H, Taniguchi H, Takada Y, Fukao K, Ohshima N: "Development of a packed-bed type bioartificial liver : improvement of the metabolic performance of the reactor and its scaling-up"Acta Hepatologica Japonica (in Japanese). 41(3). 226-227 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyoshi H, Ehashi T, Ema H, Hsu HC, Nakauchi H, Ohshima N: "Long-term culture of fetal liver cells using three-dimensional porous polymer substrate"ASAIO Journal. 46(4). 397-402 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tun T, Miyoshi H, Ema H, Nakauchi H, Ohshima N: "New type of matrix support for bone marrow cell culture : in vitro culture and in vivo transplantation experiments"ASAIO Journal. 46(5). 522-526 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyoshi H, Ohshima N: "Annual progress in artificial organs : artificial liver"The Japanese Journal of Artificial Organs (in Japanese). 29(5). 602-604 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang TH, Miyoshi H, Ohshima N: "Novel cell immobilization method utilizing centrifugal force to achieve high-density hepatocyte culture in porous scaffold"Journal of Biomedical Materials Research. 55(3). 379-386 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyoshi H, Ehashi T, Ohshima N: "Long-term three-dimensional culture of fetal liver cells to achieve prolonged metabolic functions"Tissue Engineering for Therapeutic Use 6. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tun T, Miyoshi H, Aung T, Takahashi S, Shimizu R, Kuroha T, Yamamoto M, Ohshima N: "Effect of growth factors on ex vivo bone marrow cell expansion using three-dimensional matrix support"Artificial Organs. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aung T, Miyoshi H, Tun T, Ohshima N: "Chondroinduction of mouse mesenchymal stem cells in three-dimensional highly porous matrix support"Journal of Biomedical Materials Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 柳健一, Thein Tun, 三好浩稔, 谷口英樹, 高田泰次, 深尾立, 大島宣雄: "バイオ人工肝臓への応用を目的とする充填層型リアクタの開発 : リアクタの性能改善とスケールアップ"肝臓. 41(3). 226-227 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyoshi H., Ehashi T., Ema H., Hsu HC., Nakauchi H., Ohshima N.: "Long-term culture of fetal liver cells using three-dimensional porous polymer substrate"ASAIO J. 46(4). 397-402 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tun T., Miyoshi H., Ema H., Nakauchi H., Ohshima N.: "New type of matrix support for bone marrow cell culture : in vitro culture and in vivo transplantation experiments"ASAIO J. 46(5). 522-526 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 三好浩稔, 大島宣雄: "人工肝臓 : この一年の進歩"人工臓器. 29(5). 602-604 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yang TH., Miyoshi H., Ohshima N.: "Novel cell immobilization method utilizing centrifugal force to achieve high-density hepatocyte culture in porous scaffold"J Biomed Mater Res. 55(3). 379-386 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyoshi H., Ehashi T., Ohshima N.: "Long-term three-dimensional culture of fetal liver cells to achieve prolonged metabolic functions"Tissue Engineering for Therapeutic Use. 6(in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Tun T., Miyoshi H., Aung T., et al.: "Effect of growth factors on ex vivo bone marrow cell expansion using three-dimensional matrix support"Artif Organs. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Aung T., Miyoshi H., Tun T., Ohshima N.: "Chondroinduction of mouse mesenchymal stem cells in three-dimensional highly porous matrix scaffolds"J Biomed Mater Res. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 柳健一,Thein Tun,三好浩稔,谷口英樹,高田泰次,深尾立,大島宣雄: "バイオ人工肝臓への応用を目的とする充填層型リアクタの開発:リアクタの性能改善とスケールアップ"肝臓. 41(3). 226-227 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyoshi H,Ehashi T,Ema H,Hsu HC,Nakauchi H,Ohshima N: "Long-term culture of fetal liver cells using three-dimensional porous polymer substrate"ASAIO J. 46(4). 397-402 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tun T,Miyoshi H,Ema H,Nakauchi H,Ohshima N: "New type of matrix support for bone marrow cell culture : in vitro culture and in vivo transplantation experiments"ASAIO J. 46(5). 522-526 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 三好浩稔,大島宣雄: "人工肝臓:この一年の進歩"人工臓器. 29(5). 602-604 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yang TH,Miyoshi H,Ohshima N: "Novel cell immobilization method utilizing centrifugal force to achieve high-density hepatocyte culture in porous scaffold"J Biomed Mater Res. (in press).

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-11-11  

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