| Project/Area Number |
12680837
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
OKAMOTO Kouji Kyushu Institute of Technology, Department of Biochemical Engineering and Science, Professor, 情報工学部, 教授 (40122618)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Elastin-derived peptide / Self-assembly / Elasticity / Cell migration / Cell adhesion / Anti-thrombus / 抗血栓性 / 弾性線維 / 細胞呼び寄せ型 / 弾性マトリックス / 人工血管 |
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| Research Abstract |
In 2000 to 2002, a polymer based on the repeating pentapeptide sequence Vat-Pro-Gly-Val-Gly(VPGVG) which possesses the property of self-assembly and elasticity, a polymer based on the repeating hexapeptide sequence Val-Gly-Val-Ala-Pro-Gly(VGVAPG) which possesses the property of cell migration, two random copolymers(a high VGVAPG content : hp10 and a low VGVAPG content : hp50) based on VPGVG and VGVAPG sequences described above and five random copolymers(a higher Gly-Arg-Gly-Asp-Ser(GRGDS) content : pp500 and a less GRGDS content : pp20) based on VPGVG sequence described above and GRGDS sequence which possesses the property of cell adhesion and anti-thrombus were synthesized. The chemotactic activity of hp10 and hp50 was tested and as results, hp10 was more active than hp50. The cell adhesive activity of pp20-500 was tested and as results, pp500 was more active than pp20. In 2003, two random copolymers(hp 100 and hp500) in which both copolymers are higher contents of VGVAPG than hp10 and hp50 were synthesized and tested their abilities for self assembly and chemotactic activity. As results, compared with hp10 and hp50, both hp100 and hp500 did not exhibited higher abilities of self-assembly and chemotaxis. Further studies on design of bioelastic and chemotactic matrix for artificial brood vessel is under investigation.
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