| Project/Area Number |
12680841
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Nara Medical University |
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Principal Investigator |
YOSHIKAWA Takafumi Nara Med, Univ. Assoc. Prof., 医学部, 講師 (90275347)
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| Co-Investigator(Kenkyū-buntansha) |
UEMURA Toshimasa NAIR, Senior Researcher, 主任研究官
MISHIMA Kenji Nara Med. Univ. Research Associate, 医学部, 助手 (50275343)
YAMADA Eiji Nara Med. Univ. Assistant Poof., 医学部, 助教授 (00111899)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | tissue engineering / gene / bone / wound / therapy / regeneration |
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| Research Abstract |
Culture of bone marrow blood produces bone marrow mesenchymal cells that adhere to the bottom of the culture dish. These cells are known to include cells that can differentiate in various directions. Culturing these marrow mesenchymal cells with differentiation factors and biomaterials can achieve regeneration of bone and soft tissue. The present study showed that culture of marrow mesenchymal cells with porous artificial bone material in an osteogenic culture medium containing dexamethasone could produce new bone tissue on the artificial material. This bioartificial bone graft substitute could effectively regenerate bone tissue when it was implanted in vivo. Porous artificial bone material perfused with marrow mesenchymal cells also markedly stimulated bone regeneration using gene engineering technique. Similarly, when an artificial dermis made of collagen sponge was perfused with marrow mesenchymal cells and used to cover a skin defect, the regeneration of blood vessels was stimulated. Since the present results demonstrated the ability of marrow mesenchymal cells to regenerate bone and soft tissue, these cells are expected to be useful for tissue regeneration therapy.
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