• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

SYNTHESIS AND EVALUATION OF OLIGOPEPTIDES EXHIBITING CELL-ATTACHMENT ACTIVITY FOR TISSUE ENGINEERING USE

Research Project

Project/Area Number 12680854
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionOSAKA INSTITUTE OF TECHNOLOGY

Principal Investigator

HIRANO Yoshiaki  OSAKA INSTITUTE OF TECHNOLOGY, FACULTY OF ENGINEERING, ASSOCIATE PROFESSOR, 工学部, 助教授 (80247874)

Co-Investigator(Kenkyū-buntansha) OKA Masahito  OSAKA PREFECTURE UNIVERSITY, RESEARCH INSTITUTE FOR ADVANCE SCIENCE AND TECHNOLOGY, ASSOCIATE PROFESSOR, 先端科学研究所, 助教授 (70203966)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsCell-attachment oligopeptides / RODS / RODS mimetic peptide / Peptide-immobilized materials / Conformational analysis / ECEPP / Inhibition of platelet aggregation / Structure-activity relationship / 細胞接着性オリゴペプチド / RGDSミメチィックペプチド / 生医学材料
Research Abstract

Arg-Gly-Asp-Xaa (RGDX) sequence is a cell-adhesion motif present in several matrixassociated adhesive glycoproteins including fibronectin, vitronectin, and fibrinogen. The RGDX sequence is recognized by several integrins, including the platelet, fibroblast cell, and so on. It has been demonstrated that soluble peptides containing the RGD sequence compete with RGDcontaining insoluble matrix proteins for binding to their respective integrins, and thus prevent cellmatrix adhesion. However, the affinity of the short synthetic peptides to their corresponding integrins is lower than that of proteins. In this work, we designed and synthesized Arg-Gly-AspSer (RGDS) mimetic peptides for the purpose of improving the cell attachment activity of RGDS oligopeptide, and their cell-attachment activities were assayed by L929 fibroblast cell toward peptide-immobilized polymeric materials and platelet aggregation inhibition method. Then we discussed on the structure and activity relationship of RGDS mim … More etic peptides.
RGDS and its mimetic peptides, and RGDS containing β -sheet model peptides were synthesized using liquid ancl solid phase procedures. All peptides were characterized by NMR, MALDI-TOF MS, amino acid analysis, and elemental analysis. Cell-attachment activities of these peptides were examined by cell- attachment test using L929 fibroblast cell toward peptide-immobilized PVA film.
Number of L929 cells attaching to RGDS mimetic peptide-immobilized PVA films at incubation times of 1, 3, 6 and 24 hr. The cell-attachment activity of Har-Gly-Asp-Ser (hRGDS) and Orn-Gly-Asp-Ser (OrGDS) was the same as that of RGDS. However, the cell attachment activity of Arg-Nip-Asp-Ser (RNiDS) was higher than that of RGDS from the initial stage, and cell attachment activity of RNiDS was about eight times as high as that of RGDS after incubation for 24hr. The hRGDS and OrGDS peptides present the same CD spectra as that ofRGDS. The spectral patterns suggested that these peptides possess a type I-β bend. However, RNiDS takes a typical type II-β bend. RNiDS peptide takes the different type of turn structure in comparison with the other RGDS mimetic peptides. It seems that RNiDS forms the optimum conformation for binding to the integrin receptor. It is necessary that for the active expression, the RGDS mimetic peptides take the optimum conformation in the PVA film surface. The nipecotic acid residue of RNiDS appears to contain an important structural motif that contributed to the observed cell-attachment activity. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Y.HIRANO 他4名: "Synthesis and Evaluation of Cell-attachment Activity of RGDS Mimetic Peptides"Biomedical Materials Research in the Far East. 4. 108-109 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.OKA, Y.HIRANO 他1名: "Theoretical Conformational Analysis of Oligopeptides Containing Amino-Acid Sequence Relating to Cell Attachment Activity"Biomedical Materials Research in the Far East. 4. 114-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.HIRANO 他3名: "Synthesis and Conformational Analysis of Model Polypeptide Having Repetitve Gly-Pro-Xaa Sequences"Biomedical Materials Research in the Far East. 4. 150-151 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.OKA, Y.HIRANO 他4名: "Theoretical Conformational Analysis on the Turn Portion of Tachyplesin I Based on the Ala-Residue Approximation"Peptide Science. 1999. 339-342 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.HIRANO 他4名: "Synthesis and Conformational Analysis of Model Peptides Having Repetitive Xaa-Pro-Pro Sequences"Peptide Science. 1999. 343-346 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.TABATA, Y.HIRANO, 他5名: "De Novo Formation of Adipose Tissue by Controlled Release of Basic Fibroblast Growth Factor"Tissue Engineering. 6. 279-289 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.ISHII, Y.HIRANO, 他3名: "Influence of gelatin complexation on cell proliferation activity and proteolytie resistance of basic fibroblast growth factor"J.Biomaterials Science Polymer Edition. 11. 517-582 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.HIRANO 他5名: "Synthesis of Arg-Gly-Asp-Ser Mimetic Oligopeptides and Evaluation of Their Cell-Attachment Activity"Peptide Science. 2000. 333-336 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.HIRANO 他4名: "Conformational Analysis of Model Polypeptides Having Repetitive Xaa-Pro Sequences"Peptide Science. 2000. 337-340 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.HIRANO 他4名: "Structure and Functionally Relationship of RGDS Mimeticpeptides-immobilized Biomaterials"Biomedical Materials Research in the Far East. 5. 535-536 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.IUCH, Y.HIRANO 他4名: "Cell-Attachment Activities of Surface Immobilized RGDS Mimetic Oligopeptides"Peptide Science. 2001(印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshiaki HIRANO, Tsuyoshi YAMAGUCHI, Tadashi IUCHI, Masahito OKA, Toshio HAYASHI: "Synthesis and Evaluation of Cell-attachment Activity of RGDS Mimetic Peptides"Biomedical Materials Research in the Far East. 4. 108-109 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masahito OKA, Yoshiaki Hirano, Toshio Hayashi: "Theoretical Conformational Analysis of Oligopeptides Containing Amino-Acid Sequence Relating to Cell Attachment Activity"Biomedical Materials Research in the Far East. 4. 114-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masahiro HATTORI, Masahito OKA, Toshio HAYASHI, Yoshiaki HIRANO: "Synthesis and Conformational Analysis of Model Polypeptide Having Repetitve Gly-Pro-Xaa Sequences"Biomedical Materials Research in the Far East. 4. 150-151 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masahito Oka, Masahiro Hattori, Toshio Hayashi, Yoshiaki Hirano: "Theoretical Conformational Analysis on the Turn Portion of Tachyplesin I Based on the Ala-Residue Approximation"Peptide Science. 1999. 339-342 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshiaki Hirano, Masahito Oka, Masahiro Hattori, Toshio Hayashi: "Synthesis and Conformational Analysis of Model Peptides Having Repetitive Xaa-Pro-Pro Sequences"Peptide Science. 1999. 343-346 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasuhiko TABATA, Manabu MIYAO, Takashi INAMOTO, Toshihiro ISHII, Yoshiaki HIRANO, Yoshio YAMAOKI, Yoshito IKADA: "De Novo Formation of Adipose Tissue by Controlled Release of Basic Fibroblast Growth Factor"Tissue Engineering. 6-3. 279-289 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Toshihiro Ishii, Yoshiaki Hirano, Md. Munirezzaman, Yoshito Ikada, Yasuhiko Tabata: "Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor"J. Biomaterials Science Polymer Edition. 11-6. 571-582 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshiaki Hirano, Tadashi Iuchi, Mitsutaka Kayahara, Keiko Sato, Masahito Oka, Toshio Hayashi: "Synthesis of Arg-Gly-Asp-Ser Ser Mimetic Oligopeptides and Evaluation of Their Cell-Attachment Activity"Peptide Science. 2000. 333-336 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshiaki Hirano, Masashi Shimoda, Masahito Oka, Toshio Hayashi: "Conformational Analysis of Model Polypeptides Having Repetitive Xaa-Pro Sequences"Peptide Science. 2000. 337-340 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshiaki Hirano, Tadashi IUCHI, Mitsutaka KAYAHARA, Keiko SATO, Masahito OKA, Toshio Hayashi: "Structure and Fanctionally Relationship of RGDS Mimetic Peptide Immobilized Biomaterials"Biomedical Materials Research in the Far East. 5. 535-536 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tadashi Iuchi, Mitsutaka Kayahara, Keiko Sato, Yoshiaki Hirano, Masahito Oka, Toshio Hayashi: "Cell-Attachment Activities of Surface Immobilized RGDS Mimetic Oligopeptides"Peptide Science. (in printing).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.HIRANO, 他5名: "Synthesis of Arg-Gly-Asp-Ser Mimetic Oligopeptides and Evaluation of Their Cell-Attachment Activity"Peptide Science. 2000. 333-336 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.HIRANO, 他4名: "Conformational Analysis of Model Polypeptides Having Repetitive Xaa-Pro Sequences"Peptide Science. 2000. 337-340 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.HIRANO, 他4名: "Structure and Functionally Relationship of RGDS Mimeticpeptides-immobilized Biomaterials"Biomedical Materials Research in the Far East. 5. 535-536 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] TIUCH, Y.HIRANO, 他4名: "Cell-Attachment Activities of Surface Immobilized RGDS Mimetic 0ligopeptides"Peptide Science. 2001(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Hirano 他5名: "Sythesis of Arg-Gly-Asp-Ser Mimetic Oligopeptides and Evaluation of Their Cell-Attachment Activity."Peptide Science. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Hirano 他4名: "Conformational Analysis of Model Polypeptides Having Repetitive Xaa-Pro Sequences."Peptide Science. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Hirano 他4名: "Synthesis and Evaluation of Cell-Attachment Activity of RGDS mimetic Peptides."Biomedical Materials Research in the Far East. 4. 108-109 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Oka,Y.Hirano 他1名: "Theoretical conformational Analysis of Oligopeptide Containing Amino-Acid Sequence Relating to Cell-Attachment Activity"Biomedical Materials Research in the Far East. 4. 32-33 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi