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Efficient development of new Japanese pear cultivars with high functions by genetic engineering methods

Research Project

Project/Area Number 12794002
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field 園芸・造園学
Research InstitutionTottori University

Principal Investigator

TANABE Kenji  Faculty of Agriculture, Piofessor, 農学部, 教授 (40032106)

Co-Investigator(Kenkyū-buntansha) OTANI Hiroshi  Faculty of Agriculture, Professor, 農学部, 教授 (50032305)
ITAI Akihiro  Faculty of Agriculture, Associate Professor, 農学部, 助教授 (10252876)
TAMURA Fumio  Faculty of Agriculture, Professor, 農学部, 教授 (50217197)
TABIRA Hiroki  Tottori Horticultural Research Center, Researcher, 生物工学研究室, 室長(研究職)
KODAMA Motoichiro  Faculty of Agriculture, Assistant professor, 農学部, 講師 (00183343)
村田 謙司  鳥取県園芸試験場, 果樹研究室, 室長
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥17,100,000 (Direct Cost: ¥17,100,000)
Fiscal Year 2002: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2001: ¥11,600,000 (Direct Cost: ¥11,600,000)
KeywordsStorage ability / Self-compatibilty / Black spot resistance / Pear scab resistance / Gene diagnosis / Ethylene / 黒斑病抵抗性 / MAS (Marker Assisted Selection) / ニホンナシ / 新品種育成 / 黒斑病 / DNAマーカー / 毒素結合タンパク質
Research Abstract

Tottori University researchers succeeded to release two self-compatible cultivars, 'Akibae' and 'Zuishu', which were resistant to black spot disease and had high quality. Moreover they released an early maturing cultivar 'Shinju', which were also resistant to black spot disease and had high quality. These cultivars already have been registered, and are open to public. Especially the cultivar, 'Akibae' deserved high reputation and its cultivated area increases to 40ha in 2003. They crossed these cultivars with 'Kinchaku', which is resistant to pear scab to produce new cultivars with resistance to pear scab. Shelf life of Japanese pear fruit is correlated with maximum ethylene production. They succeeded to develop the gene diagnosis for storage ability in Japanese pear cultivars and strains by analysis of genes for ethylene biosynthetic pathway. This diagnosis makes it possible to release new cultivars with high shelf life. With regard to mechanisms of black spot disease, two candidate receptor proteins for AK toxin from Alternaria alternata were identified. A trial for the gene cloning of the AK receptor protein is in progress. Researchers of Tottori Horticultural Research Center developed the gene diagnosis for self-compatibility. This also makes it easy to find new cultivars without artificial pollination.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] Itai, A., T.Kotaki, K.Tanabe, F.Tamura, O.Kawaguchi, M.Fukuda: "Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers"Theoretical and Applied Genetics. 106. 1266-1272 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., K.Tanabe, F.Tamura, M.Tomomitsu: "Cloning and characterization of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase (PPACS3) from ripening fruit of Japanese pear (Pyrus pyrifolia Nakai)"Journal of Japanese Society for Horticultural Science. 72. 99-106 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., K.Tanabe, F.Tamura: "The mechanism of ethylene production during fruit ripening in Asian pears"Acta Horticulturae. 587. 497-504 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., K.Yoshida, K.Tanabe, F.Tamura: "Expression analysis of JPRXYL gene during fruit ripening in several pear cultivars"Acta Horticulturae. 596. 183-186 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Teng, Y., K.Tanabe, F.Tamura, A.Itai, A.: "Genetic relationships of Pyrus species and cultivars native to east Asia revealed by randomly amplified polymorphic DNA markers"Journal of the American Society for Horticultural Science. 127. 262-270 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Otani, H., M.Kodama, K.Kohmoto: "Infection mechanism of black spot pathogen in Japanese pear"Acta Horticulturae. 587. 623-630 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Singh, P., Nakajima, H., Otani, H., Kodama, M., Kohmoto, K., Park, R, Bugiani, R., Cavanni: "Host-specific SV-toxin of the fungal pathogen causing brown spot of European pear"Acta Horticulturae. 596. 507-511 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, F., K.Tanabe, A.Itai: "Regulation of endodormancy in Japanese pear"Acta Horticulturae. 587. 325-336 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Teng, Y., K.Tanabe, F.Tamura, A.Itai, A.: "Assessment of genetic relatedness among large-fruited pear cultivars native to east Asia using RAPD markers"Acta Horticulturae. 587. 139-146 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Teng, Y.K.Tanabe, F.Tamura, A.Itai, A.: "Genetic relationships of pear cultivars in Xinjiang ; China, as measured by RAPD markers"Journal of Horticultural Science and Biotechnology. 76. 771-779 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Johnson, L.J., Johnson, R.D., Akamatsu, H., Salamiah, A., Otani, H., Kohmoto, K., Kodama, M.: "Spontaneous loss of a conditionally dispensable chromosome from the Alternaria alternata apple pathotype leads to loss of toxin production and pathogenicity."Current Genetics. 40. 65-72 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, F., K.Tanabe, A.Itai, M.Morimoto: "Variation in the chilling requirement for breaking leaf bud endodorrnancy in wild pear species and pear cultivars"Journal of Japanese society for Horticultural Science. 70. 596-598 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., K.Tanabe, F.Tamura, T.Tanaka: "Isolation of cDNA clones corresponding to the genes expressed during fruit ripening in Japanese pear (Pyrus pyritolia Nakai) -Involvement of ethylene signal transduction pathway in their expression"Journal of Experimental Botany. 51. 1163-1166 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Singh, R, Park, R, Bugiani, R., Cavanni, P., Nakajima, H., Kodama, M., Otani, H., Kohmoto, K.: "Effects of host-selective SV-toxin from Stemphylium vesicarium, the cause of brown spot of European pear plants, on ultrastructure of leaf cells"Journal of Phytopathology. 148. 87-93 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Otani, H.: "Host recognition by plant pathogens and role of host-specific toxins"Journal of General Plant Pathology. 66. 278-280 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., T.Kotaki., K.Tanabe, F.Tamura, D.Kawaguchi and M.Fukuda.: "Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers"Theor. Appl. Genet.. 106. 1266-1272 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., K.Tanabe, F.Tamura and M.Tomomitsu: "Cloning and characterization of a cDNA encoding 1-aminocyclopropane-1- carboxylate (ACC) synthase (PRAC33) from ripening fruit of Japanese pear (Pyrus pyrifolia Nakai)"J. Japan. Soc. Hart. Sci.. 72. 99-106 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., K.Tanabe and F.Tamura: "The mechanism of ethylene production during fruit ripening in Asian pears"Acta Hort.. 587. 497-504 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., K.Yoshida, K.Tanabe and F.Tamura: "Expression analysis of JPRXVL gene during fruit ripening in several pear cultivars"Acta Hort.. 596. 183-186 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Teng, Y., K.Tanabe, F.Tamura and A.Itai: "A Genetic relationships of Pyrus species and cultivars nativeeast Asia revealed by randomly amplified polymorphic DNA markers"J. Amer. Soc. Hort. Sci.. 127. 262-270 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Otani, H., M.Kodama and K.Kohmoto: "Infection mechanism of black spot pathogen in Japanese pear"Acta. Hort.. 587. 623-630 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Singh, P., Nakajima, H., Otani, H., Kodama, M., Kohmoto, K., Park, P., Bugiani, R., and Cavanni: "Host-specific SV-toxin of the fungal pathogen causing brown spot of European pear"Acta Hort.. 596. 507-511 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura,F., K.Tanabe and A.Itai: "Regulation of endodormancy in Japanese pear"Acta. Hort.. 587. 325-336 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Teng, Y., K.Tanabe, F.Tamura and A.Itai: "A Assessment of genetic relatedness among large-fruited pear cultivars native to east Asia using RAPD markers"Acta Hort.. 587. 139-146 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Teng, Y., K.Tanabe, F.Tamura and A.Itai: "A Genetic relationships of pear cultivars in Xinjiang ; China, as measured by RAPD markers"J. Hort. Sci. Biotech.. 76. 771-779 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Johnson, L.J., Johnson, R.D., Akamatsu, H., Salamiah, A., Otani, H., Kohmoto, K. and Kodama, M: "Spontaneous loss of a conditionally dispensable chromosome from the Altemaria alternata apple pathotype leads to loss of toxin production and pathogenicity"Curr. Genet.. 40. 65-72 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, F., K,Tanabe, A.Itai and M.Morimoto: "Variation in the chilling requirement tor breaking leaf bud endodorrnancy in wild pear species and pear cultivars"J. Japan. Soc. Hort. Sci.. 70. 596-598 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., K.Tanabe, F.Tamura and T.Tanaka: "Isolation of cDMA clones corresponding to the genes expressed during fruit ripening in Japanese pear (Pyrus pyrifolia Nakai) -Involvement of ethylene signal transduction pathway in their expression"J. Exp. Bot.. 51. 1163-1166 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Singh, P., Park, P., Bugiani, R., Cavanni, P., Nakajima, H., Kodama, M., Otani, H. and Kohmoto, K: "Effects of host-selective SV-toxin from Stemphylium vesicarium, the cause of brown spot of European pear plants, on ultrastructure of leaf cells"J. Phytapath. 148. 87-93 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Otani, H.: "Host recognition by plant pathogens and role of host-specific toxins"J.General Plant Pathol.. 66. 278-280 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Itai, A., T.Kotaki, K.Tanabe, F.Tamura, D.Kawaguchi, M.Fukuda: "Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers"Theoretical and Applied Genetics. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Itai, A., K.Tanabe, F.Tamura, M.Tomomitsu: "Cloning and characterization of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase (PPACS3) from ripening fruit of Japanese pear (Pyrus pynfolia Nakai)"Journal of Japanese Society for Horticultural Science. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Itai, A., K.Tanabe, F.Tamura: "The mechanism of ethylene production during fruit ripening in Asian pears"Acta Horticulturae. 587. 497-504 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Itai, A., K.Yoshida, K.Tanabe, F.Tamura: "Expression analysis of JPRXYL gene during fruit ripening in several pear cultivars"Acta Horticulturae. 596. 183-186 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tamura, F., K.Tanabe, A.Itai: "Regulation of endodormancy in Japanese pear"Acta Horticulturae. 587. 325-336 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Teng, Y., K.Tanabe, F.Tamura, A.Itai, A: "Genetic relationships of Pyrus species and cultivars native to east Asia revealed by randomly amplified polymorphic DNA markers"J.Amer.Soc.Hort.Sci.. 127. 262-270 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tamura, F., K.Tanabe, A.Itai, M.Morimoto: "Variation in the chilling requirement for breaking leafbud endodormancy in wild pear species and pear cultivars"J. Japan. Soc. Hort. Sci.. 70. 596-598 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Teng, W., K.Tanabe, F.Tamura, A.Itai: "Genetic relationship of pear cultivars in Xinjiang, China, as measured by RAPD makers"Journal of Horticultural Science & Biotechnology. 76. 771-779 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Teng, W., K.Tanabe, F.Tamura, A.Itai: "Genetic relationship of Pyrus species and cultivars native East Asia revealed by randomly amplified polymorphic DNA markers"J. Amer. Soc. Hort. Sci.. 127(In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Otani, H., M.Kodama, K.Koumoto: "Infection mechanism of black spot pathogen in Japanese pear"Acta Hortic.. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watanabe, H., H.Tabita, K.Murata: "Reduction of spray application times by introduction of new desease-tolerant cultivar "Gold nijisseiki" Japanese pear growing"Acta Hortic.. (In press). (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 児玉 基一朗, 赤松 創, 尾谷 浩: "Alternaria alternata 病原菌群における宿主特異的毒素生産と病原性の分子遺伝"植物感染生理談話会論文集. 37. 115-128 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Itai,A.,K.Tanabe,F.Tamura and T.Tanaka: "Isolation of cDNA clons corresponding to the genes expressed during fruit ripening in Japanese pear (Pyrus pyritolia Nakai)"J.Expt.Bot.. 51. 1163-1166 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Johnson RD.,Johnson L.,Ito,Y.,Kadama M,Otai,H,and Kohmoto,K.: "Cloning and characterization of a cyclic peptide synthetase gene from Alternaria alternata applle pathotype whose product is in volved in AM-toxin systhesis and pathogenicity."Mol Plant-Microbe Interact.. 13. 742-753 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Johnson,RP,Johnson L.,Kohmoto,K,Otani H Lane,C.R.and Kodama.: "A polymerase-chain-reaction based method to specifically detect Alternaria alternata appla pathotype (A. mali), the causal agent of Alternaria blotch of apple"Phytopathology. 90. 973-976 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Singh,P,Park,P.,Bugiani,R,Caranni.P,Nakajima.H,Kodama,M,Dtahi,H.and Kohmaok: "Effects of host-Selective SV-toxin from Stemphylium vesicarium, the cause of brown spot of European pear plants, on altrastructure of leaf cells."J.Phytopathology. 148. 87-93 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Itai,A.,K Yoshida,K.Tanabe and F.Tamura: "Expression analysis of JPRXYL gene during fruit ripening in several pear cultivats"Acta Hort. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sulamiah,Fukumasa-Nakai,Y.,Akamatsu,H.Otani,H.,Kahmoto,K.and Kodama M.: "Genetic analysis of pathogenicity and host-specific toxin production of Alternaria alternata tomato pathotype by protoplast fusion"J.Gen Plant Pathol. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Otahi,H.,Kohnohe,A.,Norita,M,Shiomi,H.Kodamo,Mand Kohmoto K: "Delivery and Perception of Pathogen Signals in Plant (N.Keen et al Eds)"APS press, St Paul. 68-76 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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