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Study of cultured bone or cartilage regeneration using human mesenchymal stem cells

Research Project

Project/Area Number 12794015
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionNagoya Univeristy

Principal Investigator

UEDA Minoru  Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (00151803)

Co-Investigator(Kenkyū-buntansha) ISHIGURO Naoki  Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20212871)
HATA Ken-ichro  University Hospital, Associate Professor, 医学部附属病院, 助教授 (80293710)
MIZUTANI Hideki  National Nagoya Hospital, Medical Chief, 医長 (30167663)
TAKAI Osamu  Center for Integranted Research in Science and Engineering, Professor, 教授 (40110712)
岩田 久  名古屋大学, 医学部, 教授 (90023796)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥37,000,000 (Direct Cost: ¥37,000,000)
Fiscal Year 2002: ¥20,900,000 (Direct Cost: ¥20,900,000)
Fiscal Year 2001: ¥16,100,000 (Direct Cost: ¥16,100,000)
KeywordsCultured Bone / Cultured cartilage / graft materials / Mechanical Stress / Mesenchymal Stem Cells / Culture on a large scale / Cultured periosteum / ティッシュエンジニアリング / 大量培養法 / ヒト
Research Abstract

Recently, it is obvious that mesenchymal stem cells (MSCs) differentiate various human tissues and these cells proliferation technology comes true. On the other hand, biodegradable matrix with differentiation and proliferation of the cells developed by rapid development of matrix engineering. On the basis of these technology, tissue engineering, which involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaffolds and growth factor, was the focus of attention. By using this method, we can regenerate a living tissue using patioents' self. These can cure the difficult treatment of chronic bone and joint disease. At first, we pay attention to develop graft materials as a matrix and improve calcium phosphate. It called CPC and was compared to β-TCP. And in animal study with osteogenesis, it was clear that the materials had good ability of bone regeneration. Therefore we can expect application of the bone materials. And applying fibrin glue and platelet rich plasma, we can confirm that these material have ability of good bone regeneration. As for cell culture method, we can get acceleration of the cell function using stress, such as stretch stress, pressure stress and supersonic waves and apply bone regeneration. There were not big differences in function of chondrocytes, which differentiated from MSCs, using the same stress. And as for culture method on a large scale using a bioreactor, it was useful under using pressure stress. In the future, by applying these results, we would like to suggest cell providing method in regenerating bone and cartilage using MSCs and strategy of fostering new industry.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (64 results)

All Other

All Publications (64 results)

  • [Publications] Hirotaka, Maeda: "Preparation of Bioactive Polylactic Acid Composites Containing Calcium Carbonates"Key Engineering Materials. 240・242. 163-166 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirotaka Maeda: "Biomimetic apatite formation on poly (lactic acid) composites containing calcium carbonates"Journal of Materials Reserch Society. 17・4. 727-730 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saisei Fu: "Experimental study of tissue engineered bone using biodegradable ceramic B -TCP as scaffold"Dentistry in Japan. 38. 67-72 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue engineering in research in oral implant surgery"Artificial Organs. 25・3. 164-171 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Hard and soft tissue engineering : application in implant surgery"Tissue Engineering for Therapeutic Use 5. 191-202 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Keisuke Wada: "Maxillary sinus floor augmentation in rabbits : A comparative Histologic-Histomorphometric study between rhBMP-2 and autogenous bone"The international Journal of Periodontics and Restorative Dentistry. 21・3. 253-263 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue engineering : applications for maxillofacial surgery"Materials Science and Engineering. 13. 7-14 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaki Honda: "Cartilage formation by cultured chondrocytes in a new scaffold made of poly (L-Lactide-ε -caprolactone) sponge"Journal of Maxillofacial Surgery. 58. 767-775 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Megumi Matsuno: "In vitro Analysis Distraction osteogenesis"The Journal of Craniofacial Surgery. 11・4. 303-307 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 夫 才成: "生体吸収性セラミックスβ-TCPを足場に用いた培養人工骨に関する実験的研究"日本口腔外科学会雑誌. 47・1. 1-7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "新世紀の歯科医学と歯科医療 -再生歯科医療-幹細胞を使った骨再生-"日本歯科医学会誌. 21・21. 18-22 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "これからの歯科は幹細胞の時代"The Quintessence. 21・1. 47-54 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "組織工学技術を用いた骨・軟骨の再生医療"medicina. 39・3. 494-499 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "21世紀の医療として期待される再生医療に向けた取り組みと将来展望"WEB Journal. 44. 2-8 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "実用化が進む再生医療"最新医学. 57. 257-268 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山田 陽一: "注入型培養骨"Clinical Calcium. 12・2. 50-54 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "再生医学は21世紀の歯科医療をどう変えるか"東京都歯科医師会雑誌. 49・2. 47-59 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 松野 恵: "皮膚と骨"外科. 63・3. 323-327 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 藤内 祝: "再生医学における移植組織の構築法"Surgery Frontier. 8・2. 86-90 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山田 陽一: "骨形成細胞による骨再生"炎症と免疫. 9・3. 253-259 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 本田 雅規: "Tissue Engineeringによる軟骨組織再生"治療学. 35・10. 1087-1090 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "骨・軟骨と再生医療"今日の移植. 14・5. 612-618 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "再生医療概論"蛋白質 核酸 酵素. 45・13. 2257-2259 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田実: "ハーバードが動き出したティッシュエンジニアリング"Quintessence. 7・5. 718-720 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 日比野 祥敬: "生体組織工学の進歩-骨・軟骨の再生-"遺伝子医学. 4・2. 256-259 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 本田 雅規: "ティッシュエンジニアリングによる軟骨修復"蛋白質 核酸 酵素. 45・13. 2297-2300 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上田 実: "ティッシュエンジニアリングと歯科医療"歯科学報. 100・8. 6-11 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirotaca maeda: "Preparation of Bioactive Polylactic Acid Composites Containing Calcium Carbonates"Key Engineering Materials. 163-166. 240-242 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirotaka Maeda: "Biomimetic apatite formation on poly(lactic acid)composites containing calcium carbonates"Journal of Materials Research Society. 17-4. 727-730 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saisei Fu: "Experimental study of tissue engineered bone using biodegradable ceramic β-TCP as scaffold"Dentistry in Japan. 38. 67-72 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue engineering in research in oral implant surgery"Artificial Organs. 25-3. 164-171 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Hard and soft tissue engineering : application in implant surgery"Elsevier Science B.V.. 191-202 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Keisuke Wada: "Maxillary sinus floor augmentation in rabbits : A comparative Histologic-Histomorphometric study between rhBWP-2 and autogenous bone"The international Journal of Periodontics and Restorative Dentistry. 21. 254-263 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue engineering : applications for maxillofacial surgery"Materials Science and Engineering. C13. 7-14 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaki Honda: "Cartilage formation by cultured chondrocytes in a new scaffold made of poly(L- Lactide-ε-caprolactone) sponge"Journal of Maxillofacial Surgery. 58. 767-775 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Megmi Matsuno: "In vitoro Analysis Distraction osteogenesis"The Journal of Craniofacial Surgery. 11-4. 303-307 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saisei Fu: "Experimental study of tissue engineerd bone using biodegradable ceramic β-TCP as a scaffold"Japanese Journal of Oral and Maxillofacial Surgery. 47-1. 1-7 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Regenerative dental therapy : Bone regenaration using Osteogenic stem cell"Journal of The Japanese Association for Dental Science. 21-21. 18-22 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Stem cell technology in dentistry"The Quintessence. 21-1. 47-54 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Bone and Cartilage regeneration using tissue engineering"Medicina. 39-3. 494-499 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue engineering : Future view and approach toward realizing regenerative medicine"WEB Journal. 44. 2-8 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Regenerative medicine of proceeding the realization"The Medical Fashon. 57. 257-268 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoichi Yamada: "Injectable Bone"Clinical Calcium. 12-2. 50-54 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "The potential of Regenerative medicine changing the dental therapy in 21 century"Journal of The Association for Tokyo Dental Doctor. 49-2. 47-59 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Megumi Matsuno: "Tissue and Bone : Regeneration of Tissue and Bone"Surgery. 63.3. 323-327 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwai Tohnai: "Transplantation Using Tissue Engineering"Surgery Frontier. 8.2. 86-90 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoichi Yamada: "Bone Regeneration Using Osteogenic Stem Cell"Inflammation and Immunology. 9-3. 253-259 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaki Honda: "Cartilage Regeneration Using Tissue Engineering"Biomedicine & Therapeutic. 35-10. 1087-1090 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue Engineering in Bone and Cartilage"Transplant Today. 14.5. 612-618 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Regenerative medicine : introduction"Protein, Nucleotic acid and Enzyme. 45.13. 2257-2259 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Harvard research center notice Tissue engineering"Quintessence Dental implantology. 7-5. 718-720 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshitaka Hibino: "The Progress of Tissue Engineering -Regeneration of the Bone and Cartilages -"Gene and Medicine. 4.2. 256-259 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaki Honda: "Cartilage Regeneration Using Tissue Engineering"Protein, Nucleotic acid and Enzyme. 45-13. 2297-2300 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue engineering and dental therapy"Report of dentistry. 100-8. 6-11 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirotaka.Maeda: "Preparation of Bioactive Polylactic Acid Composites Containing Calcium Carbonates"Key Engineering Materials. 240・242. 163-166 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirotaka Maeda: "Biomimetic apatite formation on poly (Lactic acid) composites containing calcium carbonates"Journal of Materials Reserch Society. 17・4. 727-730 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saisei Fu: "Experimental study of tissue engineered bone using biodegradable ceramic β-TCP as scaffold"Dentistry in Japan. 38. 67-72 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 上田 実: "新世紀の歯科医学と歯科医療 -再生歯科医療-幹細胞を使った骨再生-"日本歯科医学会誌. 21・21. 18-22 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 上田 実: "これからの歯科は幹細胞の時代"The Quintessence. 211. 47-54 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 上田 実: "組織工学技術を用いた骨・軟骨の再生医療"medicina. 39・3. 494-499 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 上田 実: "21世紀の医療として期待される再生医療に向けた取り組みと将来展望"WEB Journal. 44. 2-8 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 上田 実: "実用化が進む再生医療"最新医学. 57. 257-268 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山田 陽一: "注入型培養骨"Clinical Calcium. 12・2. 50-54 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Boo, J., Ueda M.et al.: "Tissue-Engineering Bone using Mesenchymal Stem Cells and a Biodegradable Scaffold"The Journal of Craniofacial Suegery. (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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