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Cellular and molecular responses to power-frequency electromagnetic fields.

Research Project

Project/Area Number 12831003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIYAKOSHI Junji  KYOTO UNIVERSITY GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学研究科, 助教授 (70121572)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsMagnetic fields / Gene expression / HSP70 / Signal transduction / Heat shock / 極低周波変動磁場 / 変動磁場 / 姉妹染色分体交換 / 染色体異常 / DNA鎖切断 / 電離放射線
Research Abstract

We investigated the change in gene expression of the stress protein, i.e., the heat shock protein in this study, under a high intensity of extremely low frequency (ELF) magnet field, and as a positive control, we investigated the effect of heat processing. In addition, we also studied a modifying effect of the magnetic field on gene expression of the heat shock protein under treatment of combination of the magnetic field and heat processing.
1. The study on the heat shock protein was performed using HSP70, a typical heat shock protein, which reacts to numerous stresses including heat shock and expresses transiently. Goodman et al. (1997) reported ESP70 induction by ELF magnetic field exposure, however, the data at the level of μT were obtained and negative counterarguments were published. In this study, we investigated whether the exposure to a high magnetic intensity of ELF magnet field produces an effect on induction of the heat shock protein or not using human leukemia-derived cells (HL60) that Goodman et al. used. No induction of HSP70 protein expression was observed 24 hours after exposure to the 50 mT ELF magnetic field.
2. As a positive control, we investigated the effect of heat processing. In addition, we also studied a modifying effect of the magnetic field on gene expression of the heat shock protein under treatment of combination of the ELF magnetic field and heat processing. Under a mild heat shock (40 - 42 ℃), induction of HSP70 protein expression was observed in HL60 cells. After combined treatment of this heat processing and exposure to the 50 mT ELF magnetic field, the level of HSP70 protein expression was lower than that under treatment of heat shock alone, which indicated that the ELF magnetic field inhibited the HSP70 protein expression. These results suggested that a high intensity of ELF magnetic field exaggerated the negative feedback action against the HSP70 expression induced by the heat shock and inhibited the HSP70 expression.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Junji Miyakoshi: "Suppression of heat-induced HSP-70 by simultaneous exposure to 50 mT magnetic field"Life Sciences. 66. 1187-1196 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Junji Miyakoshi: "Exposure to extremely low frequency magnetic fields suppresses X-ray-induced transformation in mouse C3H10T1/2 cells"Biochemical and Biophysical Research Communications. 271. 323-327 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroko Yaguch: "Increased chromatid-type chromosomal aberrations in mouse m5S cells exposed to power-line frequency magnetic fields"International Journal of Radiation Biology. 76. 1677-1684 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Gui-Rong Ding: "Increase in hypoxanthine-guanine phosphoribosyl transferase gene mutations by exposure to electric field"Life Sciences. 68. 1041-1046 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Junji Miyakoshi: "Exposure to strong magnetic fields at power frequency potentiates X-ray-induced DNA starnd breaks"Journal of Radiation Research. 41. 293-302 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Gui-Rong Ding: "Increase in X-ray-induced mutations by exposure to magnetic field (60Hz,5mT) in NF-κB-inhibited cells"Biochemical and Biophysical Research Communications. 276. 238-243 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Junji Miyakoshi, Yukihiro Mori, hiroko Yaguchi, Guirong Ding and Akira Fujimori: "Suppression of heat-induced HSP-70 by simultaneous exposure to 50 mT magnetic field"Life Sciences. 66. 1187-1196 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Junji Miyakoshi, Masami yoshida, hiroko Yaguchi and Gui-Rong Ding: "Exposure to extremely low frequency magnetic fields suppresses X-ray-induced transformation in mouse C3H10T1/2 cells"Biochemical and Biophysical Research Communications. 271. 323-327 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroko Yaguchi, Masami Yoshida, Gui-Rong Ding, Kazushige Shingu and Junji Miyakoshi: "Increased chromatid-type chromosomal aberrations in mouse m5S cells exposed to power-line frequency magnetic fields"International Journal of Radiation Biology. 76. 1677-1684 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Gui-Rong Ding, Kanako Wake, Masao Taki and Junji Miyakoshi: "Increase in hypoxanthine-guanine phosphoribosyl transferase gene mutations by exposure to electric field"Life Sciences. 68. 1041-1046 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Junji Miyakoshi, Masami Yoshida, Keiko Shibuya and Masahiro Hiraoka: "Exposure to strong magnetic fields at power frequency potentiates X-ray-induced DNA starnd breaks"Journal of Radiation Research. 41. 293-302 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Gui-Rong Ding, Hiroko Yaguchi, Masami Yoshida and Junji Miyakoshi: "Increase in X-ray-induced mutations by exposure to magnetic field (60 Hz, 5 mT) in NF-κB-inhibited cells"Biochemical and Biophysical Research Communications. 276. 238-243 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Junji Miyakoshi: "Suppression of heat-induced HSP-70 by simultaneous exposure to 50 mT magnetic field"Life Sciences. 66. 1187-1196 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Junji Miyakoshi: "Exposure to extremely low frequency magnetic fields suppresses X-ray-induced transformation in mouse C3H10T1/2 cells"Biochemical and Biophysical Research Communications. 271. 323-327 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroko Yaguchi: "Increased chromatid-type chromosomal aberrations in mouse m5S cells exposed to power-line frequency magnetic fields"International Journal of Radiation Biology. 76. 1677-1684 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Gui-Rong Ding: "Increase in hypoxanthine-guanine phosphoribosy1 transferanse gene mutations by exposure to electric field"Life Science. 68. 1041-1046 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Junji Miyakoshi: "Exposure to strong magnetic fields at power frequency potentiates X-ray-induced DNA starnd breaks"Journal of Radiation Research. 41. 293-302 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Gui-Rong Ding: "Increase in X-ray-induced mutations by exposure to magnetic field (60Hz, 5mT) in NF-κB-inhibited cells"Biochemical and Biophysical Research Communications. 276. 238-243 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] J.Miyakoshi: "Increase in hypoxanthine-guanine phosphoribosyl transferase gene mutations by exposure to electric field."Life Sciences. 68. 1041-1046 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] J.Miyakoshi: "Increase in X-ray-induced mutations by exposure to magnetic field (60Hz,5mT) in NF-κB-inhibiteed cells."Biochem.Biophys.Res.Commun.. 276. 238-243 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] J.Miyakoshi: "Inhibition of IκB-α phosphorylation at serine and tyrosine acts Independently on sensitization to DNA damaging agents in human Glioma cells"Br.J.Cancer. 82. 28-33 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] J.Miyakoshi: "Increased chromatid-type chromosomal aberrations in mouse m5S Cells exposed to power-line frequency magnetic fields."Intl.J.Radiat.Biol.. 76. 1677-1684 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] J.Miyakoshi: "Exposure to strong magnetic fields at power frequency potentiates X-ray-induced DNA starnd breaks."Journal of Radiation Research. 41. 293-302 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] J.Miyakoshi: "Exposure to extremely low frequency magnetic fields suppresses X-ray-induced transformation in mouse C3H10T1/2 cells."Biochem.Biophys.Res.Commun.. 271. 323-327 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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