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The experimental study for the explication of mechanism and therapy on the joint contracture

Research Project

Project/Area Number 12832036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionNAGASAKI UNIVERSITY

Principal Investigator

OKITA Minoru  SCHOOL OF MEDICINE, RESEARCH ASSOCIATE, 医学部, 助手 (50244091)

Co-Investigator(Kenkyū-buntansha) FUJINO Hidemi  SUZUKA UNIVERSITY OF MEDICAL SCIENCE, ASSOCIATE PROFFESOR, 保健衛生学部, 助教授 (20278998)
YOSHIMURA Toshiro  SCHOOL OF MEDICINE, PROFFESOR, 医学部, 教授 (80182822)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsContracture / Soleus muscle / Immobilization / Muscle fiber / Collagen fiber / Hyaluronan / Passine tension / 固定期間 / 伸張運動
Research Abstract

Study 1 : The experimental study for the explication of mechanism on the joint contracture
Immobilization is often associated with a decrease in muscle elasticity. This condition is called muscle contracture, but the mechanism is not yet clear. We examined changes in ankle joint mobility, passive tension, sarcomere length, collagen fibril arrangement in the endomysium, and hyaluronic acid in muscular tissue 1, 2, 4, 8, and 12 weeks after immobilization of rat soleus muscles in shortened position. Ankle joint mobility decreased with the duration of immobilization. Passive tension on immobilized muscles had increased. Sarcomere length had shortened 1 week after immobilization, but did not show further change 2, 4, 8, and 12 weeks after immobilization. Collagen fibril arrangement in the endomysium 1 and 2 weeks after immobilization was longitudinal to the axis of the muscle fibers, whereas 4, 8, and 12 weeks after immobilization it was circumferential.
Hyaluronic acid in muscular tissue had … More increased 1 week after immobilization but remained at the same level at weeks 2, 4, 8, and 12. Histochemically, hyaluronic acid in the endomysium of immobilized muscular tissue was stained more strongly and widely than that in the control tissue. Increased hyaluronic acid in muscular tissue may induce muscle stiffness, but the significance of how hyaluronic acid is related to the mechanism of muscle contracture was not clear. The findings suggest mat muscle contracture started 1 week after immobilization and increased with the length of immobilization. Consequently, muscle contracture is affected by the shortening muscle fibers during the early stage of immobilization, after which the collagen adapts by the fibril arrangement in the endomysium becoming more circumferential. This change in collagen fibril arrangement may cause advanced muscle contracture in the late stage of immobilization.
Study 2 : The experimental study for the explication of therapy on the joint contracture
1) To determine whether prolonged stretching is preventing for joint contracture in mice. It was found that progression of joint contracture can not be prevented in periods of stretch lasting 10 min, and can prevent in periods of stretch lasting 20 min or more. But the progression of joint contracture can not completely prevented in periods of stretch lasting 30 min.
2) To determine whether prolonged stretching is ameliorating for disuse muscle atrophy in immobilized rat soleus muscles. Eighteen male Wistar rats were divided randomly into control (n=3) and immobilization (n=15) groups. The immobilization groups rats were immobilized on ankle joint for 4 weeks, animals were divided into three groups : immobilization alone (group I, n=3), stretch training for 30 min/day for 1or 3 weeks after remobilization (group S, n=6), and spontaneous recovery (non stretch training) for 1 or 3 weeks after remobilization (group NS, n=6). After 1 week of remobilization, muscle fiber diameters for type I fibers in group S had increased significantly compared with group NS. After 3 weeks of remobilization, muscle fiber diameters for types I and II fibers in group S had increased significantly compared with group NS. Our findings suggest that prolonged stretching induces recovery from disuse muscle atrophy after joint contracture. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] 佐伯 彩, 沖田 実, 吉村俊朗, 中野治郎: "弛緩位ならびに伸張位での固定がラットヒラメ筋におよぼす影響"理学療法学. 27巻. 63-68 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 沖田 実: "拘縮に伴う筋・筋膜の変化"ボバースジャーナル. 23巻. 55-60 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 沖田 実, 吉村俊朗, 中野治郎, 佐伯 彩: "拘縮の病態とストレッチング"理学療法探求. 3巻. 29-36 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okita M, Yoshimura T, Nakano J, Saeki A, Uehara A, et al.: "Effects of short duration stretching on disuse muscle atrophy in immobilized rat soleus muscles"Journal of the Japanese Physical Therapy Associasion. 4巻. 1-5 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 中田 彩, 沖田 実, 中居和代, 中野治郎, 他: "持続的伸張運動の実施時間の違いが関節拘縮の進行抑制効果におよぼす影響-マウスにおける実験的研究-"理学療法学. 29巻. 1-5 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okita M, Yoshimura T, Nakano J, Motomura M, Eguchi K: "Effects of reduced joint mobility on sarcomere length, collagen fibril arrangement in the endomysium, and hyaluronan in rat coleus muscle"Journal of Muscle Research & Cell Motility. 24巻(印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 沖田 実: "踵歩きギプス療法-heel gait cast-(穐山富太郎 編) 第1章-II「拘縮の病態」を執筆"医歯薬出版. 174 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 沖田 実: "拘縮の予防と治療(奈良勲,浜村明徳 編) 第3章「拘縮に関する基礎研究」を執筆"医学書院. 129 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saeki A, Okita M, Yoshimura T, Nakano J: "Effects of immobilization on rat soleus muscle in the shortened and lengthened position"Rigakuryohogaku. 27-3. 63-68 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okita M: "Morphological canges in muscle fibers and myofascias on the joint contracture"Journal of the Japan Bobath Association. 23-1. 55-60 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okita M, Yoshimura T, Nakano J, Saeki A: "Pathogenesis of the joint contracture and the influence of the muscle stretching on the joint contracture"Rigakuryohogaku tankyu. 3. 29-36 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okita M, Yoshimura T, Nakano J, Saeki A, Uehara A, et al: "Effects of short duration stretching on disuse muscle atrophy in immobilized rat soleus muscles"Journal of the Japanese Physical Therapy Association. 4. 1-5 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakata A, Okita M, Nakai K, Nakano J, et al.: "Effects of prolonged stretching for prevention of joint contracture in mice"Rigakuryohogaku. 29-1. 1-5 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okita M, Yoshimura T, Nakano J, Motomura M, Eguchi K: "Effects of reduced joint mobility on sarcomere length, collagen fibril arrangement in the endomysium, and hyaluronan in rat soleus muscle"Journal of Muscle Research & Cell Motility. (in press). 24 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okita M, ed by Akiyama T: "Pathomechanics for the joint contracture. Kakato Aruki Gipusu Ryoho (heel gait cast therapy)"Ishiyaku Publishers, Tokyo. 13-32 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okita M, ed by Nara I and Hamamura A: "Basic study for the joint contracture. Prevention and Therapy for the Joint Contracture"Igakushoin, Tokyo. 37-48 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 佐伯 彩, 沖田 実, 吉村俊朗, 中野治郎: "弛緩位ならびに伸張位での固定がラットヒラメ筋におよぼす影響"理学療法学. 27巻. 63-68 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] 沖田 実: "拘縮に伴う筋・筋膜の変化"ボバースジャーナル. 23巻. 55-60 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] 沖田 実, 吉村俊朗, 中野治郎, 佐伯 彩: "拘縮の病態とストレッチング"理学療法探求. 3巻. 29-36 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okita M, Yoshimura T, Nakano J, Saeki A, Uehara A, et al.: "Effects of short duration stretching on disuse muscle atrophy in immobilized rat soleus muscles"J Jpn Phys Ther Assoc. 4巻. 1-5 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中田 彩, 沖田 実, 中居和代, 中野治郎, 他: "持続的伸張運動の実施時間の違いが関節拘縮の進行抑制効果におよぼす影響-マウスにおける実験的研究-"理学療法学. 29巻. 1-5 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 藤原孝之, 鈴木重行, 小林邦彦, 沖田 実, 他: "理学療法の効果の検証-関節可動域制限の病態に対して-"理学療法学. 29巻. 344-350 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okita M, Yoshimura T, Nakano J, Motomura M, Eguchi K: "Effects of reduced joint mobility on sarcomera length, collagen fibril arrangement in the endomysium, and hyaluronan in rat soleus muscle"Journal of Muscle Research & Cell Motility. 24巻(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 沖田 実: "踵歩きギプス療法-heel gait cast-(穐山富太郎編)第1章-II「拘縮の病態」を執筆"医歯薬出版. 174 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 沖田 実: "拘縮の予防と治療(奈良勲, 浜村明徳編)第3章「拘縮に関する基礎研究」を執筆"医学書院. 129 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 沖田 実: "拘縮に伴う筋・筋膜の変化"ボバースジャーナル. 23巻・1号. 55-60 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 沖田 実, 吉村俊朗, 中野治郎, 佐伯 彩: "拘縮の病態とストレッチング"理学療法探求. 3巻. 29-36 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Okita M, Yoshimura T, Nakano J, Saeki A, Uehara A, Mineshita A, Eguchi K: "Effects of short duration stretching on disuse muscle atrophy in immobilized rat soleus muscles"Journal of the Japanese Physical Therapy Association. 4巻・1号. 1-5 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中田 彩, 沖田 実, 中居和代, 中野治郎, 田崎洋光, 他: "持続的伸張運動の実施時間の違いが関節拘縮の進行抑制効果におよぼす影響-マウスにおける実験的研究-"理学療法学. 29巻・1号. 1-5 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 沖田実: "拘縮に伴う筋・筋膜の変化"ボバースジャーナル. 23巻・1号. 55-60 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 沖田実,吉村俊朗,中野治郎,佐伯彩: "拘縮の病態とストレッチング"理学療法探求. 3巻. 29-36 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Okita M,Yoshimura T,Nakano J,Saeki A,Uehara A,Mineshita A,Eguchi K: "Effects of short duration stretching on disuse muscle atrophy in immobilized rat soleus muscles"Journal of Japanese Physical Therapy Association. 4巻・1号(印刷中). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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