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Identification of Crosstalk Mechanisms between Mechanical and Hormonal Signal Transduction Pathways in Osteocyte

Research Project

Project/Area Number 12832061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionKanagawa Dental College

Principal Investigator

MIKUNI Yuko  Kanagawa Dental College, Faculty of Dentistry, Lecturer, 歯学部, 講師 (60050689)

Co-Investigator(Kenkyū-buntansha) ITOMAN Moritoshi  Kitasato University, Faculty of Medicine, Professor, 医学部, 教授 (70104528)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsosteocytes / ultrasound / osteopenia / disuse / synergy / mechanotransduction / bone formation / crosstalk
Research Abstract

In order to confirm our previous observation in isolated osteocytes of synergy between PTH and mechanical loading, in vivo, the 30-week-old female Wistar rats, either housed in cages of limited space (RESTRAINED and RESTRAINED+PTH) or allowed free activity (CONTROL and PTH) in the institutional standard cages, were inject subcutaneously with 10 μg/kg human PTH (1-34) or saline, three times a week. Rats were sacrificed after two sc calcein injections (6 mg/kg) 13 days and 3 days before death. For 3D evaluation, whole tibia was examined by pQCT and confocal laser microscopy. At the end of 6-week experiment, RESTRAINED and PTH is significantly different (p<0.001) and the differnce, was much larger than the added differences between RESTRAINED and CONTROL (not significant) and RESTRAINED and RESTRAINED+PTH (not significant). BMD of cortical bone also showed a significant difference between the RESTRAINED and PTH (p<0.001) in a similar manner. Also in confocal micrographs, larger numbers of much brighter periosteocytic calcein labels were obserbed in PTH compared to RESTRAINED suggesting the osteocytic mineralizing activity by combined effects of PTH and daily activity of rats. A critical role of osteocytes in sensitizing bone to PTH during daily activity was suggested.
When PTH (1-34) alone or stretching alone exhibited moderate induction of mRNAs such as IGF-I or osteocalcin, we showed that stretch-loading in combination with PTH resulted in a dramatic upregulation.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Mikuni-Takagaki, Y.: "Bone Formation Induced by Pulsed Ultrasound"Bulletin Kanagawa Dental College. 30(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 高垣 裕子: "骨形成の力学的要因-骨芽細胞の分化・局在に伴う応答のバリエーションと細胞間共同作用-"日本骨形態計測学会雑誌. 12・1(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Naruse, K et al.: "Distinct anabolic response of osteoblast to low-intensity pulsed ultrasound"J. Bone Miner. Res.. 17(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mikuni-Takagaki, Y. et al.: "The role of calcium channels in osteocyte function"J Musculoskel. Neuron. Interact.. 2・3. 255-258 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satoyoshi, M. et al.: "Matrix metalloproteinase-2 in dentin matrix mineralization"J. Endodontics. 27・4. 462-466 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 高垣裕子: "骨・軟骨細胞と伸展・圧縮刺激"The Bone. 14. 529-536 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 高垣裕子(分担): "骨細胞(osteocyte)の形成と機能 in 新分子骨代謝学と骨粗鬆症"メディカルレビュー社. 512 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyauchi, A., Notoya, K., Mikuni-Takagaki, Y., Takagi, Y., Goto, M., Miki, Y., Takano-Yamamoto, T., Fujii, Y., Jinnai, K., Takahashi, K., Kumegawa, M., Chihara, K., and Fujita, T.: "Parathyroid hormone-activated volume sensitive calcium influx pathways in mechanically loaded osteocytes"J. Biol. Chem.. 275. 3335-3342 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Naruse, K., Mikuni-Takagaki, Y., Azuma, Y., Ito, M., Oota, T., Kameyama, K. and Itoman, M.: "Anabolic response of mouse-bone-marrow-derived stromal cell clone ST2 cells by low-intensity pulsed ultrasound"Biochem. Biophys. Res. Comm.. 268. 216-220 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mikuni-Takagaki, Y., Furue, M., Kawata, A., Kawase, T., Sasaguri, K., Kubota, T., Sato, S.: "Stretch-sensing Mechanisms Uniquely Expressed in Osteocytes"Bulletin Kanagawa Dental College. 28. 13-16 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satoyoshi, M., Kawata. A., Koizumi, T., Inoue. K., Itohara, S., Teranaka, T. and Mikuni-Takagaki,Y.: "Matrix metalloproteinase-2 in dentin matrix mineralization"J. Endodontics. 27. 462-466 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mikuni-Takagaki, Y., Naruse, K., Azuma Y. and Miyauchi, A.: "The role of calcium channels in osteocyte function"Musculoskel Neuron Interact. 2. 255-258 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mikuni-Takagaki, Y.: "Bone Formation Induced by Pulsed Ultrasound"Bulletin Kanagawa Dental College. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mikuni-Takagaki, Y.: "Bone Formation Induced by Pulsed Ultrasound"Bulletin Kanagawa Dental College. 30(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高垣 裕子: "骨形成の力学的要因-骨芽細胞の分化・局在に伴う応答のバリエーションと細胞間共同作用-"日本骨形態計測学会雑誌. 12・1(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Naruse, K et al.: "Distinct anabolic response of osteoblast to low-intensity pulsed ultrasound"J. Bone Miner. Res.. 17(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mikuni-Takagaki, Y. et al.: "The role of calcium channels in osteocyte function"J Musculoskel. Neuron. Interact.. 2・3. 255-258 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Satoyoshi, M. et al.: "Matrix metalloproteinase-2 in dentin matrix mineralization"J. Endodontics. 27・4. 462-466 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高垣裕子: "骨・軟骨細胞と伸展・圧縮刺激"The Bone. 14. 529-326 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高垣裕子(分担): "骨細胞(osteocyte)の形成と機能in新分子骨代謝学と骨粗鬆症"メディカルレビュー社. 512 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyauchi,A et al.: "Parathyroid hormone-activated volume sensitive calcium influx pathways in mechanically loaded osteocytes."J.Biol.Chem.. 275. 3335-3342 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Naruse,K et al.: "Anabolic response of mouse-bone-marrow-derived stromal cell clone ST2 cells by low-intensity pulsed ultrasound."Biochem.Biophys.Res.Comm.. 268. 216-220 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Satoyoshi,M. et al.: "Matrix metalloproteinase-2 in dentin matrix mineralization."J.Endodontics. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mikuni-Takagaki,Y.: "Osteocytes and Osteoporosis."Connective Tissue. 32. 303-306 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 高垣裕子: "骨・軟骨細胞と伸展・圧縮刺激"The Bone. 14. 529-536 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mikuni-Takagaki,Y. et al.: "Stretch-sensing Mechanisms Uniquely Expressed in Osteocytes"Bulletin Kanagawa Dental College. 28. 13p-16p (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 高垣裕子(分担): "骨細胞(osteocyte)の形成と機能in新分子骨代謝学と骨粗鬆症"メディカルレビュー社. 532 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Satoyoshi M. et al. (分担): "Matrix Metalloproteinase-2 in Dentinogenesis in Chemistry and Biology of Mineralized Tissues"American Academy of Orthopaedic Surgeons. 458 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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