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CLINICAL APPLICATION OF URINARY NAG INDEX AND SDS-PAGE ANALYSIS OF URINARY PROTEIN IN FELINE AND CANINE URINARY DISORDER

Research Project

Project/Area Number 12833003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionIWATE UNIVERSITY

Principal Investigator

SATO Reeko  IWATE UNIVERSITY, DEPARTMENT OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (80142892)

Co-Investigator(Kenkyū-buntansha) SYUTO Bunei  IWATE UNIVERSITY, DEPARTMENT OF AGRICULTURE, PROFESSOR, 農学部, 教授 (60001533)
NAITO Yoshihisa  IWATE UNIVERSITY, DEPARTMENT OF AGRICULTURE, PROFESSOR, 農学部, 教授 (40003785)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsURINARY NAG INDEX / SDS-PAGE / URINARY PROTEIN / NEPHROSIS MODEL / COWS / CATS / DOGS / NAG(NAGase) / 尿 / 尿蛋白 / JDS-PAGE / 腎臓病 / アイソザイム / 腎臓
Research Abstract

This study was undertaken to determine the clinical usefulness of measurement of urinary NAG index and SDS-PAGE analysis of urinary protein as a marker for urinary disorder in animals. The results were as follows :
(1) Urinary NAG index elevated from the early stage of nephrosis model experimentally-induced by potassium dichromate or sulfonamide in cattle and cat. Isoenzyme B of urinary NAG was increased after the administration of renal toxic drugs. (2) Urinary NAG index of the cases with chronic or acute renal failure was higher than that of healthy animals. On the other hand, NAG index of the cases with feline lower urinary tract disease or lower urinary tract infection was in the normal range. The dogs with lower urinary tract infection accompanied by pyelonephritis showed elevated values of NAG index. The elevation of NAG index indicated the extension of infection and inflammation to the pelvis. Therefore, measurement of urinary NAG index seemed to be useful as a marker of pyelonephritis. In secondary renal failure such as diabetes mellitus and pyometra, observation of urinary NAG index was also useful as a marker of renal injury. (3) In urine of chronic renal failure, low molecular proteins such as retinol-binding protein were detected by SDS-PAGE assay. In the assay of SDS-PAGE, serum protein pattern was observed in urine of lower urinary tract infection. SDS-PAGE assay of urinary protein was useful to distinguish the parenchymal lesions between kidneys and lower urinary tract. SDS-PAGE assay of urinary protein was more effective when it was done with the measurement of urinary NAG index. In conclusion, the clinical usefulness of measurement of urinary NAG and SDS-PAGE assay of urinary protein as the markers for renal diseases in animals were attested from the results obtained in this study.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Reeko Sato: "Function and clinical application of lactoferrin in small animals"Small Animal Clinic. 119. 12-21 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jun Sato et al.: "Three cases of congenital lymphedewa in Japanese Shorthorn cattle"J. Jpn. Vet. Med. Assoc.. 54. 95-98 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jun Sato et al.: "Pituitary chromo phobe carcinoma with a low level serum gonadotropin and an a spermatogenesis in a dog"J. Vet. Med. Sci.. 63. 183-185 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jun Yasuda et al.: "Idiopathic thrombocytopenia in Japanese Black cattle"J. Vet. Med. Sci.. 64. 87-89 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Reeko Sato et al.: "Clinical availability of urinary N-acetyl-β-D-glucosamini-dase index in dogs with urinary disease"J. Vet. Med. Sci.. 64(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Reeko Sato et al.: "Urinary excretion of N-acetyl-β-D-glucosamini-dase and its isc enzymes in cats with urinary disease"J. Vet. Med. Sci.. 64(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Reeko Sato et al.: "Lactoferrin : Structure, Function and Application"ELSEVIER. 6 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Norio Hirano et al.: "Lactoferrin : Structure, Function and Application"ELSEVIER. 5 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] REEKO SATO: "Lactoferrin : Structure, Function and Application ; Plasma lactoferrin concentration measured by ELISA in healthy and diseased cows"ELSEVIER. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] NORIO HIRANO: "Lactoferrin : Structure, Function and Application ; Antiviral effect of bovine lactoferrin on felone herpesvirus infection"ELSEVIER. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] REEKO SATO: "Function and clinical application of lactoferrin in small animals"Small Animal Clinic. 119. 12-21 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] NORIO YAMAGISHI: "Effect of oral administration of rumen-bypass vitamin D and Ca metabolism in cows"J. Vet. Med. Sci.. 62. 403-408 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] JUN SATO: "Three cases of congenital lymphedema in Japanese Shorthorn cattle"J. Jpn. Vet. Med. Assoc.. 54. 95-98 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] JUN SATO: "Pituitary chromophobe carcinoma with a low level serum gonadotropin and an aspermatogenesis in a dog"J. Vet. Med. Sci.. 63. 183-185 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] JUN YASUDA: "Idiopathic thrombocytopenia in Japanese Black cattle"J. Vet. Med. Sci.. 64 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] REEKO SATO: "Clinical availability of urinary N-acetyl-β- D-glucosaminidase index in dogs with urinary diseases"J. Vet. Med. Sci.. 64 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] REEKO SATO: "Urinary excretion of N-acetyl-β-D-glicosaminidase and its isoenzymes in cats with urinary disease"J. Vet. Med. Sci.. 64 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] JUN SATO: "High levels of serum alkaline phosphatase activities and its isoenzymes in canine cases of pyometra"J. Jpn. Vet. Med. Assoc.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Reeko Sato: "Function and Clinical appiication of Factoferrin in small animals"Small Animal Clinic. 119. 12-21 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jun Sato et. al.: "Pituitary chromophobe carcinoma with a low level serum gonadotropin and anaspermatogenesis in a dog"J. Vet. Med. Sci. 63. 183-185 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Reeko Sato et. al.: "clinical availability of urinary N-acetyl-B-D glucosaminidase index in dogs with urinary diseases"J. Vet. Med. Sci. 64(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Reeko Sato et. al.: "Urinary excretion of N-acetyl-B-D-glucosaminidase and its isoengymes in cats with urinary disease"J. Vet. Med. Sci. 64(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jun Yasuda et. al.: "Idiopathic thrombocgtopenia in Japanease Black cattle"J. Vet. Med. Sci. 64. 87-89 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jun Sato et. al.: "High levels of serum alkaline phosphatase activities and its isoengymes in canine cases of pygmetra"J. Vet. Med. Assoc. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamagishi N. et al.: "Effect of oral administration of Rumen-bypass Vitamin D3 on Vitamine Dand Ca metabolism in cows"J.Vet.Med.Sci.. 62. 403-408 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 佐藤淳 他: "日本短角種牛における先天性リンパ水腫の3例"日本獣医師会雑誌. 54・2. 95-98 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Reeko Sato. et al.: "Lactoferrin : Structure, Function and Application"ELSEVIER. 6 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Norio Hirano et.al.: "Lactoferrin : Structure, Function and Application"ELSEVIER. 5 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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