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Aging of the dog brain : molecular pathological study on the mechanism of biometal (iron) accumulation

Research Project

Project/Area Number 12833006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionTottori University

Principal Investigator

SHIMADA Akinori  Tottori Univ., Agriculture, Prof., 農学部, 教授 (20216055)

Co-Investigator(Kenkyū-buntansha) SATOH Masahiko  National Inst. Environ. Sci., Researcher, 環境健康研究領域健康指標研究室, 主任研究員 (20256390)
YAMANO Yoshiaki  Tottori Univ., Agriculture, Associate Prof., 農学部, 助教授 (00182593)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsDog / Brain / Aging / Iron / Metallothionein / 8-OHdG / 8-OHdG / フェリチン
Research Abstract

Purpose of the study
Age-related changes of the brain of dogs including neuronal degeneration induced by the free radical intoxication were pathologically studied. The study included relationship between the iron accumulation and neuronal degenerative changes and mechanism of the iron accumulation in the brain. 1. Distribution of the age-related morphological changes, accumulated iron (cell species, electron microscopic investigation), 2. Demonstration of iron-related proteins (ferritin, transferrin), 3. 8-OHdG and 4-HNE expression as a marker of cell injury induced by free radicals, 4. Metallothionein expression (immunohistochemical, biochemical analysis).
Summarized findings and conclusion
1. Iron accumulation was demonstrated in the cytoplasm of glial cells (oligodendroglia), some evidence of iron accumulation in the mitochondria.
2. Ferritin distribution was similar to that of iron accumulation in the cytoplasm of glial cells ; ferritin immunoreactivity became strong with age.
3. 8-OHdG and 4-HNE expression as a marker of cell injury induced by free radicals, increased with age suggesting that oxygen radicals would, in part, be responsible for brain aging.
4. Level of total Metallothionein decreased with age in both gray and white matter, indicating that shortage of the anti-peroxidization protein would be, in part, responsible for the brain aging.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Shimada A., Kohno E., Maeda M., Morita T.: "Spatial and temporal changes of iron, ferritin and transferrin in the brain of aged dog"Toxicol. Sci. Supplement. 60. 360 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimada A., Maeda M., Mizutani Y., Sawada M., Morita T.: "Immunohistochemical and in situ hybridization study of metallothionein (i & ii, iii) expression in the brain of aged dog"Toxicol. Sci. Supplement. 66. 12 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimada A., Kohno E., Maeda M., Morita T.: "Spatial and temporal changes of iron, ferritin and transferrin in the brain of aged dog"Toxicol. Sci. Supplement.. 60. 360 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimada A., Maeda M., Mizutani Y., Sawada M., Morita T.: "Immunohistochemical and in situ hybridization study of metallothionein ( i & ii, iii) expression in the brain of aged dog"Toxicol. Sci. Supplement.. 66. 12 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimada A., Kohno E., Maeda M., Morita T.: "Spatial and temporal changes of iron, ferritin and transferrin in the brain of aged dog"Toxicol. Sci. Supplement. 60. 360 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimada,A.: "Metallothionein expression and its Significance in the brain aging of dog"Trace Elements in Man and Animals. 10. 444-445 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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