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INCOORDINATION AND LOCALIZATION OF BORNA DISEASE VIRUS (BDV) IN THE BRAIN OF HORSES WITH BDV PERSISTENT INFECTION

Research Project

Project/Area Number 12833008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionRAKUNO GAKUEN UNIVERSITY

Principal Investigator

TANIYAMA Hiroyukl  VETERINARY SCHOOL, RAKUNO GAKUEN UNIVERSITY, ASSO. PROF., 獣医学部, 助教授 (90133800)

Co-Investigator(Kenkyū-buntansha) HAGIWARA Katsurou  VETERINARY SCHOOL, RAKUNO GAKUEN UNIVERSITY, ASSI. PROF., 獣医学部, 講師 (50295896)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsBORNA DISEASE VIRUS / HORSE / BRAIN / INCOORDINATION / IN SITU PCR / ボルナ病ウイルス / in situ hybridaization / in situ RT-PCR
Research Abstract

1.To investigate the prevalence of diseases in the Borna disease virus (BDV) antibody positive race horses, we undertook seroepidemiological studies of BDV infection on 125 culled race horses in Hokkaido, Japan. The serological study disclosed the presence of antibodies only to BDV- p40 or -p24 in 19.2 % (24/125) and 3.2 % (4/125) of culled horses, respectively. Antibodies to both BDV -p40 and -p24 were found in 24.0 % (30/125) of these horses. Of particular note was the finding that locomotorium disorders were detectable at a significantly higher rate in BDV antibody positive horses than that in the seronegative horses. These results imply that BDV infection may possibly contribute to an increase in the incidence rate of locomotorium disorders in racehorses.
2.Three central nervous systems collected from three horses with clinical wobbler disease and the Borna disease virus (BDV) positive antibody were investigated histopathologically. Immunohistochemical and in situ PCR examinations were performed for BDV antigens and BDV-RNAs. In their cerebellums, severe glial fibrosis was observed in the medulla and partly cortex. However, inflammatory changes such as perivascular cell infiltration with lymphocytes and macrophages were not observed in any areas. BDV antigens (p40 and p24) in the neuron and neuropil were not observed with immunohistochemical examinations. In situ PCR methods were revealed the minimal presence of BDV-RNAs in the cytoplasm and nuclei of a few number of neuron.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Okamto M, et al.: "Borna Disease in a Dog in Japan"J Comp Pathol.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okamoto M, et al.: "Borna disease in a heifer in Japan"Vet Rec.. 150(1). 16-18 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hagiwara K, et al.: "Nosological study of Borna disease virus infection in race horses"Vet Microbiol.. 84(4). 367-374 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniyama H, et al.: "Equine borna disease in Japan"Vet Rec.. 148(15). 480-482 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe M. et al.: "Neurological diseases and viral dynamics in the brains of neonatally borna disease virus-infected gerbils"Virology.. 282(1). 65-76 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura Y, et al.: "Isolation of Borna disease virus from human brain tissue"J Virol.. 74(10). 4601-4611 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hagiwara K, et al.: "Detection of Borna disease virus in a pregnant mare and her fetus"Vet Microbiol.. 72(3-4). 207-216 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okamoto M. et al.: "Borna Disease in Dog in Japan"J Comp Pathol.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okamoto M. et al.: "Borna disease in a heifer in Japan"Vet. Rec.. 50 (1). 16-18 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hagiwara K. et al.: "Nosological study of Borna disease virus infection in race horses"Vet. Microbiol.. 84 (4). 367-3 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniyama H, et al: "Equine borna disease in Japan"Vet. Rec.. 148 (15). 480-482 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe M. et al: "Neurological diseases and viral dynamics in the brains of neonatally borna disease virus-infected gerbils"Virology. 282 (1). 65-76 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura Y, et al: "lsolation of Borna disease virus from human brain tissue."J.Virol. 74 (10). 4601-4611 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hagiwara K. et al: "Detection of Borna disease virus in a pregnant mare and her fetus"Vet. Microbiol.. 72 (3-4). 207-216 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okamoto M, et al.: "Borna Disease in a Dog in Japan"J Comp Pathol.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Okamoto M, et al.: "Borna disease in a heifer in Japan"Vet Rec.. 150(1). 16-18 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hagiwara K, et al.: "Nosological study of Borna disease virus infection in race horses"Vet Microbiol.. 84(4). 367-374 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Taniyama H, et al.: "Equine borna disease in Japan"Vet Rec.. 148(15). 480-482 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watanabe M, et al.: "Neurological diseases and viral dynamics in the brains of neonatally borna disease virus-infected gerbils."Virology.. 282(1). 65-76 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hagiwara K,Kamitani W,Takamura S,Taniyama H,Nakaya T,Tanaka,H,Kirisawa R,Iwai H,Ikuta K.: "Detection of Borna disease virus in a pregnant mare and her fetus."Vet.Microbiol.. 15;72(3-4). 207-216 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakamura Y,Takahashi H,Shoya Y,Nakaya T,Watanabe M,Tomonaga K,Iwahashi K,Ameno K,Momiyama N,Taniyama H,Sata T,Kurata T, de la Torre JC,Ikuta K.: "Isolation of Borna disease virus from human brain tissue."J Virol.. 74(10). 4601-4611 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Taniyama H,Okamoto M,Hirayama K,Hagiwara K,Kirisawa R,Kamitani W,Tsunoda N,Ikuta K.: "Equine borna disease in Japan.Vet Rec.2001"Vet.Rec.. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Okamoto M,Maei K,Murakami N,Kamitani W,Hagiwara K,Kirisawa R,Iwai H,Hirayama K,Moniyama N,OOnuki S,Tagami M,Tsunoda N,Ikuta K,Taniyama H.: "Spontaneous Borna disease of racing horeses in Japan : Histologic, immunohitochemical and molecular biological studies of the central nervous system."J Vet Med Sci.. (In press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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