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Development of Oxidized LDL Receptor (CD36) Knockout Mice and Its Application to Elucidation of Molecular Mechanism for Atherogenesis

Research Project

Project/Area Number 12835005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionOsaka University

Principal Investigator

YAMASHITA Shizuya  Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (60243242)

Co-Investigator(Kenkyū-buntansha) HIRANO Ken-ichi  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (30332737)
SAKAI Naohiko  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (80294073)
TOMIYAMA Yoshiaki  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (80252667)
西田 誠  大阪大学, 医学部・附属病院, 医員
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsCD36 / Atherosclerosis / Knockout mice / Oxidized LDL / 受容体 / 粥状動脈硬化 / 長鎖脂肪酸 / スカベンジャー受容体
Research Abstract

CD36 is a membrane glycoprotein expressed on platelets, monocytes/macrophages and adipose tissues. CD36 belongs to the class B scavenger receptor family. We found patients with CD36 deficiency in 1990 and analyzed the pathophysiology of these patients.
We have identified 3 novel mutations in the CD36 gene and reported that macrophages from CD36-deficient patients showed a reduced capacity to bind oxidized LDL and were resistant to foam cell formation. Thus, CD36 may be one of the important receptors for oxidized LDL. Furthermore, we have found that CD36 is also expressed in cardiac muscles of CD36-positive subjects, but not in those of CD36-deficient patients.
The deficiency of CD36 may lead to the high frequency of cardiomyopathy in these patients.
Little has been known as to the in vivo effect of CD36 deficiency. We found that CD36-deficient patients are accompanied by insulin resistance, which is a common phenotype of multiple risk factor syndrome. In the current study, we have used genetic engineering techniques to establish CD36 knockout mice and elucidate the role of CD36 in the pathogenesis of atherosclerosis.
We have established chimera mice and have obtained homozygotes of CD36 knockout mice. We have performed a backcross breeding and are currently trying to see the changes of serum lipids, glucose metabolism and the presence or absence of insulin resistance in these mice. We will finally examine the susceptibility of CD36 knockout mice to atherosclerosis.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] S.Yamashita, et al.: "Low HDL syndromes and high HDL syndrome"The encyclopedia of endocrinology and endocrine disease. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Yamashita, et al.: "Expression of functional analyses of novel mutations in ATP-binding cassette transporter-1 in Japanese patients with high density lipoprotein deficiency"Biochem Biophys Res Commun. 290. 713-721 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Yamashita, et al.: "ATP-binding cassette transporter-1 (ABCA1) induces rearrangement of actin cytoskeletons through possible interaction between ABCA1 and Cdc42"Biochem Biophys Res Commun. 287. 757-765 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Yamashita, et al.: "CD36 deficiency is associated with insulin resistance"Lancet. 357. 686-687 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Yamashita, et al.: "Insulin resistance and CD36 deficiency"Lancet. 358. 244 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Yamashita, et al.: "Expression of cholesteryl ester transfer protein in human atherosclerotic lesions and its implication in the reverse cholesterol transport"Atherosclerosis. 159. 67-75 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Yamashita, et al: "Low HDL syndromes and high HDL syndrome"The encyclopedia of endocrinology and endocrine disease. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Yamashita, et al: "Expression of cholesteryl analyzes of novel mutations in ATP-binding cassette transporter-1 in Japanese patients with high density lipoprotein deficiency"Biochem Biophys Res Commun. 290. 713-721 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Yamashita, et al.: "ATP-binding cassette transporter-1 (ABCA1) induces rearrangement of actin cytoskeletons through possible interaction between ABCA1 and Cdc42"Biochem Biophys Res Commun. 287. 757-765 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Yamashita, et al.: "CD36 deficiency is associated with insulin resistance"Lancet. 357. 686-687 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Yamashita, et al.: "Insulin resistance and CD36 deficiency"Lancet. 358. 244 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Yamashita, et al.: "Expression of cholestery1 ester transfer protein in human atherosclerotic lesions and its implication in the reverse cholesterol transport"Atherosclerosis. 159. 67-75 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita S, et al.: "Low HDL syndromes and high HDL syndrome"The encyclopedia of endocrinology and endocrine dlsease. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashita S, et al.: "Expression of functional analyses of novel mutations in ATP-binding cassette transporter-1 In Japanese patlents with high density lipoprotein ldeliciancy"Biochem Biophys Res Commun. 290. 713-721 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashita S, et al.: "ATP-binding Cassette Transporter-1(ABCA1)Induces Rearrangement of Actln Cytoskeletons through Possible interaction between ABCA1 and Cdc42"Biochem Biophys Res Commun. 287. 757-765 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashita S, et al.: "CD36 deficiency is associated with insulin resistance"Laucet. 357. 686-687 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashita S, et al.: "Insulin resistance and CD36 deficiency"Laucet. 358. 244 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashita S, et al.: "Expression of cholesteryl estor transfer protein In human atherosclerotic leslons and its impllcation in the reverse cholesterol transport"Athowsclevosis. 159. 67-75 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Miyaoka,T.Kuwasako et al: "CD36 deficiency associated with insulin resistance"Lancet. 357. 686-687 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Janabi,S.Yamashita et al: "Oxidized LDL-induced NF-kB activation and subequent expression of proinflammatory genes are defective in monocyte-derived macrophages from CD36-deficient patients"Arterioscler Thromb Vasc Biol. 20. 1953-1960 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Nozaki,S.Yamashita et al.: "CD36 mediates long-chain fatty acid transport in human myocardium : complete myocardial accumulation defect of radiolabeled long-chain fatty acid analog in subjects with CD36 deficiency"Mol Cell Biochem. 192. 129-135 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Nakata,M.Nishida et al.: "CD36, a novel receptor for oxidized low density lipoproteins, is highly expressed on lipid-laden macrophages in human atherosclerotic aorta"Arterioscler Thromb Vasc Biol. 19. 1333-1339 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Hirano S.Yamashita et al.: "Expression of human scavenger receptor class B type I (SR-BI) in cultured human monocyte-derived macrophages and atherosclerotic lesions"Circ Res. 8. 108-116 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nakagawa,,S.Yamashita et al.: "Oxidized LDL increases and interferon-g decreases expression of CD36 in human monocyte-derived macrophages"Arterioscler Thromb Vasc Biol. 18. 1350-1357 (1998)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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