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Spermatotoxicity and Transgenerational Effects of Dioxin

Research Project

Project/Area Number 12836006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionOsaka University

Principal Investigator

NAKAJIMA Hiroo  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (20237275)

Co-Investigator(Kenkyū-buntansha) RYO Haruko  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (90301267)
NOMURA Taisei  Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (90089871)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsDioxins / Mouse / Reproductive toxicity / Teratogenicity / Transgenerational effects / Environmental Contamination / Genetic Effects
Research Abstract

We examined the morphological, functional and molecular abnormalities in the offspring of mice (B6C3F_1 and CB.17F_1) exposed to dioxins.
A single i.p. or p.o. administration of 100 or 50 ng of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin per g of body weight was given to male mice, and mated with untreated female mice to examine the morphological and functional abnormalities in their offspring. On Day 18 of gestation, their fetuses and fetal appendages were removed by the cesarian operation. Implants, early deaths, late deaths, and the external abnormality of living fetuses were examined, and fetuses were resuscitated by pudding with tissue paper to check RDS (respiratory distress syndrome).
Up to now, we have gotten following results ; the incidence of implantation decreased significantly in the dioxin (100ng/g) treated group in contrast to control group. And significant increase of morphological anormalies (p<0.05) was observed in CB.17F_1 mice, but in B6C3F_1, an increase was marginal significant (p=0.055). The malformations observed in the dioxin treated group were dwarf, omphalocele, agnathy, cleft palate, kinky tail, short tail, agenesis of the testis, general edema, syndacryly, and in the control group were dwarf, Meckel diverticulum, open eye, and cleft palate.
Additionally, dose dependency (increasing incidence of early deaths, malformation and decrease of implantation, living fetus) was also observed in B6C3F_1 mice.
Furthermore, significant increase of functional defects like respiratory distress syndrome was observed in the F_1 fetuses of TCDD treated male mice of two different strains.
Increased incidence of molecular changes, i. e., length polymorphism of repetitive DNA sequences (pc-3) was detected but it was not yet significant at present. Large scale experiments are in progress.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Nakajima, H., et al.: "Anomalous (Preduodenal) Portal Vein : Autosomal Recessive Mutation in AKRI/JMice"Comparative Medicine. 51. 23-26 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nair, C.K.K., et al.: "Radioprotectors in Radiotherapy"J. Radiat. Res.. 42. 21-37 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hongyo, T., et al.: "Specific c-kit Mutations in Sinonasal NK/T-Cell Lymphoma in China and Japan"Cancer Res.. 60. 2345-2347 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nomura, T.: "Dose and dose rate effect in mutagenesis, teratogenesis and carcinogenesis"Radiation and Homeostasis. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Baskar, R., et al.: "Spontaneous and radiation induced tumorigenesis in p53 deficient mice"Radiation and Homeostasis. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniguchi, H., et al.: "Effects of N-methyl-N'-nitro-N-nitrosoguanidine on the human colorectal polyps consecutively maintained in SCID mice"Cancer Lett.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, H., et al.: "Biological Effects of Low Dose Radiation"Inst. Environmental Sciences. 249 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, H., Li, L. Y., Nomura, T.: "Anomalous (Preduodenal) Portal Vein : Autosomal Recessive Mutation in AKR/J Mice"Comparative Medicine. 51. 23-26 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hongyo, T., Nomura, T., et al.: "Specific c-kit Mutations in Sinonasal NK/T-Cell Lymphoma in China and Japan"Cancer Res. 60. 2345-2347 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, H., Li, L. Y., Nomura, T: "Biodosimetry for detecting low dose and loe doce rate radiation"Biological Effects of Low Dose Radiation Inst. Environmental Sciences. 199-205 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nair, C. K. K., et al.: "Radioprotectors in Radiotherapy"J. Radiat. Res. 42. 21-37 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nomura, T.: "Dose and dose rate effect in mutagenesis, teratogenesis and carcinogenesis"Radiation and Homeostasis. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Baskar, R., et al.: "Spontaneous and radiation induced tumorigenesis in p53 deficient mice"Radiation and Homeostasis. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniguchi, E., et al.: "Effects of N-methyl-N'-nitrosoguanidine on the human colorectal polyps consecutively maintained in SCID mice"Cancer Lett.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakajima, H., et al.: "Anomalous(Preduodenal)Portal Vein : Autosomal Recessive Mutation in AKR/J Mice"Compar ative Medicine. 51. 23-26 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 野村大成: "放射線研究と動物実験施設"アニテックス. 13. 185-190 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hongyo, T., et al.: "Specific c-kit Mutations in Sinonasal NK/T-Cell Lymphoma in China and Japan"Cancer Res.. 60. 2345-2347 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakajima, H., et al.: "Biological Effects of Low Dose Radiation"Inst. Environmental Sciences. 249 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakajima,H., et: "Comparative Medicine"Anomalous (Preduodenal) Portal Vein : Autosomal Recessive Mutation in AKR/J Mice. 51. 23-26 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hongyo,T., et al.: "Specific c-kit Mutations in Sinonasal NK/T-Cell Lymphoma in China and Japan."Cancer Res.. 60. 234-234 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakajima,H., et: "Biological Effects of Low Dose"Inst. Environmental. 249 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nomura,T.: "Biological Effects of Low Dose"Inst. Environmental. 249 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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