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Study on the effect of endocrine disruptor on memory, learning and emotion in mice

Research Project

Project/Area Number 12836008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionFaculty of Human Development

Principal Investigator

ENOMOTO Taira  Kobe University, Faculty of Human Science, Professor, 発達科学部, 教授 (00127622)

Co-Investigator(Kenkyū-buntansha) NISHIZAKI Tomoyuki  Kobe University, Hyogo College of Medicine, Professor, 医学部, 教授 (00221474)
MATSUMOTO Akira  Kobe University, Graduate School of Medicine, Associate Professor, 医学系研究科・医学部, 助教授 (80181759)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordsnell2 knockout mice / nell2 gene / learning and memory / LTP / beta-amiloid / hippocampus / 回避学習 / 海馬 / ne112遺伝子 / マウス / 環境ホルモン / 記憶 / 学習 / 情動行動 / 性行動
Research Abstract

In order to study the effect of endocrine disrupter on learning, memory and emotion in mice, we have established knockout mouse strain defective to nel12 gene which is expressed mainly in hippocampus in mouse brain. Using this system, we have studied the LTP(Long-term potentiation) and hippocampal dependent memory and learning of normal, and knockout mice in water-maze system. Furthermore, we have studied expression and distribution of carboxypeptidase B in the subregions of the hippocampus of normal and Alzheimer's human brain.
Result :
1) Nell2 gene knockout mice has revealed normal behavior in water maze compared with normal mice : both normal and knockout mice require 4〜6 trials for learning water maze. On the other hand, the induction of physiological LTP in hippocampus was much higher in knockout mice than normal mice, indicating that LTP system in knockout mice become more sensitive.
2) In avoiding learning system, knockout mice have learned to avoid electrical stimulation faster t … More han normal mice. Further, knockout mice have the electrical stimulation in memory longer than normal mice. These results indicate that knockout mice may be potentiated in certain leaning and memory system such as avoiding learning.
3) During the study of nel12 gene knockout mice , we have found that the nel12 gene knockout mice are male-specifically defective in fertilization. All knockout female were pregnant in mating with +/+,-/+, -/- male, respectively. On the other hand, all knockout male were defective in fertilization with +/+,-/+, -/- female. These results clearly indicate that knockout of nel12 gene induces male-specific unfertilization.
4) In order to study the mechanism of male-specific unfertilization, we have stdudied sperm formation, sperm motility, sperm morphology, and genes related to sperm-egg binding. Sperm in knockout mice was normal in its motility, number and morphology, indicating that sperm formation is normal in knockout mice.
5) We have also studied gene expression of sperm-egg binding factors such as fertilin and cyritestin in normal and knockout mice. Both genes were expressed normally in knockout mice testis. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] S.Matsuyama: "Activation of nikotine acetylcholine receptors induces lon-term potentiation in vivo in the intact mouse dentate gyrus"Eur. J. Neurosci.. 12. 3741-3747 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Nomura: "A long-lasting facilitation of Hippocampal neurotransmission via a phospholipase A2 signaling pathway"Life Sciences. 68. 2885-2891 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Papp: "Expression and distribution of carboxypeptidase B in the hippocampal subregions of normal and alzheimer's disease brain"Acta Biologica Hungarica. 52(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Matsuyama, A. Matsumoto, T. Enomoto and T. Nishizaki: "Activation of nicotine acetylcholine receptors induces long-term potentiation in vivo in the intact mouse dentate gyrus"European Journal of Neuroscience. 12. 3741-3747 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Nomura, T. Nishizaki, T. Enomoto, and H. Itoh: "A long-lasting facilitation of hippocampal neurotransmission via a phosphilipase A2 signaling pathway"Life Science. 68. 2885-2891 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Papp, I. Torok, A. Matsumoto, T. Enomoto, S. Matsumoto and P. Kasa: "Expression and distribution of carboxypeptidase B in the hippocampal subregions of normal and Alzheimer's dissease brain"Acta Biologica Hungarica. 52, No.2 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Matsuyama: "Activation of nikotine acetylcholine receptors induces lon-term potentiation in vivo in the intact mouse dentate gyrus"Eur. J. Neurosci. 12. 3741-3747 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Nishizaki: "Presynaptic nicotinic receptors play a critial role in a pathway of NMDA receptor-dependent LTP"Life Science. 68. 2885-2891 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Torok.H.Papp: "Expression and distribution of carboxypepetidase B in the hippocampal subregions of normal and Alzheimer's disease brain"Acta Biol. Hun.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 榎本平: "内分泌かく乱物質問題:女性ホルモン様物質の動態への発生影響"神戸大学発達科学部研究紀要. 7. 141-153 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Matsuyama: "Activation of nicotinic acetylcholine receptors induces long-term potentiation in vivo in the intact mouse dentate gyrus"European Journal of Neuroscience. 12. 3741-3747 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nomura: "A long-lasting facilitation of hippocampal neurotransmission via a phospholipase A2 signaling pathway"Brain Research. (In press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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