| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | antifeedant / Cryptomeria japonica / Acuta despesta / (-)-cubebol / (+)-2.7(14), 10-bisabolatrien-1-ol-4-one / sequirin-C / agathresinol / (-)-cubebol / (+)-2, 7, (14), 10-bisabola trien-1-01-4-one / Seguirin-C / agathresino / Acusta despesta / (+)-2,7(14),10-bisabolatrien-1-ol-4-one |
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| Research Abstract |
During our studies on the components in Japanese cedar, Cryptomeria japonica, we found that the crude methanol extract of C. japonica showed intense antifeeding activity against one snail species, Acusta despesta, which is well-known as a pest of many vegetables and crops. The active components in the extract were separated into the hexane and ethyl acetate soluble fractions. From the active ethyl acetate soluble fraction, two norlignans, sequirin-C and agatharesinol, were isolated and identified as the active compounds. Both compounds inhibited feeding behavior of A. despesta at 30 μg/cm2 and 40 μg/cm2 concentrations, respectively, when applied by an eggplant leaf or filter paper containing-20 μl of 5 % sucrose solution.
On the other hand, (-)-cubebol and (+)-2,7(14),10-bisabolatrien-1-ol-4-one were isolated and identifed as the active compounds from the hexane fr. Both compounds strongly inhibited the feeding behavior of A. despesta at 120 μg/cm2 and 80 μg/cm2 concentrations, respectively. In-addition to these, the absolute configuration of (+)-2,7(14), 10-bisabolatrien-1-ol-4-one was also determined as (IS, 6R)-2,7(14),10-bisabolatrien-1-ol-4-one.
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