| Project/Area Number |
12839014
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物資源の変換と展開
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KOBAYASHI Genta Faculty of Agriculture, Assistant Prof., 大学院・農学研究院, 助手 (40291512)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASAKI Koji Faculty of Engineering Science, Prof., 大学院・総合理工学研究院, 教授 (30154769)
SONOMOTO Kenji Faculty of Agriculture, Prof., 連合農学, 教授 (10154717)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Biodiesel / Acetone-butanol fermentation / Shochu distillery waste / Food waste / Clostridium / Used cooking oil / Extract fermentation / Environment |
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| Research Abstract |
The possibility of employing methylated crude palm oil (CPOE) and methylated used cooking oil (UCOE) as an extractant to reduce end-product inhibition and to enhance solvent productivity in acetone-butanol-ethanol (ABE) fermentation was evaluated using oleyl alcohol as the standard butanol extractant. Using CPOE as the extraction solvent, about 47 % of the total butanol produced was extracted, glucose consumption was increased to 83 % and relatively high glucose consumption rates and solvent productivities were obtained. Butanol production increased to 20.9 g/l; total ABE solvents and yield also increased from 21.2 g/l and 38 % (in conventional fermentation) to 29.8 g/l and 40.4 %, respectively. The partition coefficient of CPOE to butanol was approximately 0.9 and the butanol partition coefficient of UCOE was similar to that of CPOE. Shochu distillery wastewater and food waste were also employed as substrates in ABE fermentation. Utilizing only shochu distillery wastewater as substrate, butanol production was 3.5 g/l. However, when shochu distillery wastewater and food waste were used in combination, butanol production and total ABE solvents yield were 11.6 g/l and 57.8 %, respectively.
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