Maf遺伝子導入による転移能の変化

• Project/Area Number: 12877283
• Research Category: Grant-in-Aid for Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Pediatric surgery
• Research Institution: National Institute of Radiological Sciences (2001-2002); University of Tsukuba (2000)
• Principal Investigator: 岩川 眞由美 独立行政法人, 放射線医学総合研究所・フロンティア研究センター, グループリーダー (30231723)
• Co-Investigator(Kenkyū-buntansha): 本橋 ほづみ 筑波大学, 先端学際領域研究センター, 講師 (00282351)
• Project Period (FY): 2000 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥1,600,000 (Direct Cost: ¥1,600,000); Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
• Keywords: Maf / C-1300 / Maf遺伝子 / C-1300神経芽腫 / リポフェクチン法 / liver colony法

Research Abstract

C-1300神経芽腫にリポフェクチン法を用いてMaf遺伝子を導入し、A/Jマウスに静注移植、転移実験を行った。転移臓器別のアッセイ法としては、肝ではliver colony法で検討した。Maf遺伝子導入法を確立し、transfection効率を一定とした。静注移植後、肝転移を定量的に得ることができた。Maf遺伝子導入群では転移抑制が見られた。
次に、Small Maf memberであるMafKをT-cell系で過剰発現するtransgenic mouseを開発した。このtransgenic mouseの寿命は短く、T-cell系の減少、IL-2、IL-4のmRNAレベルの低下を検討した。

Research Products

• [Publications] Katsuoka F, Motohashi H, et al.: "Small Maf compound mutants display central nervous system neuronal degeneration, aberrant transcription, and Bach protein mislocalization coincident with myoclonus and abnormal startle response"Mol Cell Biol.. 23(4). 1163-1174 (2003)
• [Publications] Motohashi H, O'Connor T, et al.: "Integration and diversity of the regulatory network composed of Maf and CNC families of transcription factors"Gene. 294(1-2). 1-12 (2002)
• [Publications] 岩川眞由美, 大川治夫 他: "腎芽腫の病期・年齢による治療戦略と予後"小児外科. 34(10). 1174-1179 (2002)
• [Publications] Terunuma T, Hara A, Senarita M, Motohashi H, Kusakari J: "Effect of acoustic overstimulation on regulation of glucocorticoid receptor mRNA in the cochlea of the gu nea pig"Hear Res.. 151. 121-124 (2001)
• [Publications] Yoh K, Sugawara T, Motohashi H, et al.: "Transgenic over-expression of Mafk suppressesT cell proliferation and function in vivo"Genes Cells. 6. 1055-1066 (2001)
• [Publications] Kusunoki H, Motohashi H, Katsuoka F, et al.: "Solution structure of the DNA-binding domain of Maf G"Nature Struct. Biol.. (in press).
• [Publications] Motohashi,H.: "Perinatal Synthetic Lethality and Hematopoietic Defects in Compound mafG :: mafK Mutant Mice."EMBO J. 19. 1335-1345 (2000)
• [Publications] Hoshino,H.: "Oxidative Stress Abolishes Leptomycin B-sensitive Nuclear Export of Transcription Repressor Bach2 that Counteracts Activation of Maf Recognition Element."J.Biol.Chem.. 275. 15370-15376 (2000)
• [Publications] Katsuoka,F.: "One enhancer mediates mafK transcriptional activation in both hematopoietic and cardiac cells."EMBO J.. 19. 2980-2991 (2000)
• [Publications] Motohashi,H.: "Positive or negative MARE-dependent transcriptional regulation is determined by the abundance of small Maf proteins."Cell. 103. 865-875 (2000)