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寄生植物ストライガのトランスクリプトーム解析

Research Project

Project/Area Number 12F02781
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field 植物分子生物・生理学
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

白須 賢  独立行政法人理化学研究所, 環境資源科学研究センター, グループディレクター

Co-Investigator(Kenkyū-buntansha) SPALLEK Thomas  独立行政法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員
SPALLEK Thomas  独立行政法人理化学研究所, 植物免疫研究グループ, 外国人特別研究員
Project Period (FY) 2012 – 2014
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2014: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2013: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2012: ¥400,000 (Direct Cost: ¥400,000)
KeywordsParastic Plants / RNA-Sequencing / Striga / Pectin-methyl-esterase Inhibitor / 植物 / 病理学 / 生理学 / 遺伝子
Outline of Annual Research Achievements

I developed two projects to elucidate molecular interaction between host plants and parasitic plants. I focus thereby of three different parasitic plants, Striga asiatica, Striga hermonthica and Phtheirospermum japonicum.
1) I characterized of a novel protein family of secreted proteins, which are conserved in at least three parasitic plants species and carry a Plant invertase/pectin methylesterase inhibitor domain. My new results show that members of this protein family specifically localized to haustoria at late stages of infection and that expression of these genes in non-parasitic plants caused to severe developmental phenotypes. These phenotypes maybe associated with altered cell wall composition or nutrient acquisition.
2) I studied host hormone responses during plant parasitism and discovered an involvement of host auxin signalling components in infection and haustoria development. I established a method to evaluate Arabidopsis infection by S. asiatica, S. hermonthica and P. japonicum and screened a collection of known Arabidopsis auxin signalling mutants for resistance against these parasitic plants. I discovered that mutants along the Solitary Root (SLR) pathway exhibited elevated levels of resistance. This work showed that parasitic plants couple infection processes to host components such as auxin signalling.

Current Status of Research Progress
Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

本研究課題は平成26年度が最終年度のため、記入しない。

Strategy for Future Research Activity

本研究課題は平成26年度が最終年度のため、記入しない。

Report

(3 results)
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (4 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] The genus Striga : a witch profile2014

    • Author(s)
      Thomas Spallek, Mutuku Musembi, Ken Shirasu
    • Journal Title

      Molecular Plant Patholology

      Volume: 14 Issue: 9 Pages: 861-869

    • DOI

      10.1111/mpp.12058

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Trans-family grafting between parasitic plants and their hosts (talk)2014

    • Author(s)
      Thomas Spallek
    • Organizer
      Plant reprogramming workshop
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2014-11-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] GENOME AND TRANSCRIPTOME ANALYSES OF STRIGA SPP.2014

    • Author(s)
      Satoko Yoshida, ri-ichiroh Manabe, Seunglill Kim, Thomas Spallek, Musembi Mutuku, Michael Timko, Doil Choi, Ken Shirasu
    • Organizer
      XVI International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      Rhodes, Greece
    • Year and Date
      2014-07-06 – 2014-07-10
    • Related Report
      2014 Annual Research Report
  • [Book] Mc Graw-Hill Year Book of Science (Lexical entry)2014

    • Author(s)
      Musembi Mutuku, Thomas Spallek, Ken Shirasu
    • Publisher
      Striga : physiology, effects, and genomics
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-04-25   Modified: 2024-03-26  

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