Application of Hydrophobic Interaction among Biomolecules under Extreme Conditions to Strongly-correlated Softmaterials

• Project/Area Number: 13031024
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: NAKAMURA Satoshi Tokyo Tech, Bioeng, Professor, 大学院・生命理工学研究科, 教授 (50227899)
• Project Period (FY): 2001 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥52,000,000 (Direct Cost: ¥52,000,000); Fiscal Year 2003: ¥25,900,000 (Direct Cost: ¥25,900,000); Fiscal Year 2002: ¥15,800,000 (Direct Cost: ¥15,800,000); Fiscal Year 2001: ¥10,300,000 (Direct Cost: ¥10,300,000)
• Keywords: Bioengineering / Biotechnology / Softmaterial / Protein / Extreme environment

Research Abstract

The objective of this research is to elucidate the importance of non-covalent bonds in proteins on tolerances towards extreme environments. A 2Fe-2S feredoxin from triangular disc-shaped extremely halophilic archaeon Haloarcula japonica was investigated. It seemed that the additional amino terminal region of H. japonica Fd was importantfor halotolerancy. The thermostability of 3-isopropylmalate dehydrogenase of moderate facultative thermophile Bacillus coagulans was improved by amino acid substitutions at the subunit-subunit interface and/or a loop region.Furthermore, the function of chitin-binding domain of a multi-domain chitinase from alkaliphilic Bacillus sp. J813 was elucidated.

Research Products

• [Publications] T.Matsuo et al.: "Cloning and Expression of the Ferredoxin Gene from Extremely Halophilic Archaeon Haloarcula japonica Strain TR-1"BioMetals. 14. 135-142 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] R.Yatsunami et al.: "A Novel uhitosanase trom bacillus sp. Strain K17 : Gene Cloning and Expression in Escherichia coli"Nucleic Acids Res.Suppl.. 2. 227-228 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Komatsu et al.: "Thermostable Synthetic Hemoprotein"Chem.Lett.. 32. 108-109 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ihsanawati et al.: "Crystallization and Preliminary X-ray Studies of Xylanase 10B from Thermotoga maritima"Acta Cryst.. D59. 1659-1661 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Inami et al.: "Directed Evolution of Xylanase J from Alkaliphilic Bacillus sp. Strain 41M-1"Nucleic Acids Res.Suppl.. 3. 315-316 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] S.Nakamura: "Structure and Function of a Multidomain Alkaline Xylanase from Alkalinhilic Bacillus sp. Strain 41M-1"Catal.Survey Asia. 7. 157-164 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Fukazawa et al.: "Chitosan 7B-degrading Enzyme from Alkaliphilic Bacillus sp. Strain J813 "Chitin and Chitosan, Chitin and Chitosan in Life Science" (Ed. by T. Uragami et al.)"Kodansha. 428-429 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Matsuo: "Cloning and Expression of the Ferredoxin Gene from Extremely Halophilic Archaeon Haloarcula japonica Strain TR-1"BioMetals. 14. 135-142 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] R.Yatsunami: "A Novel Chitosanase from Bacillus sp. Strain K17 : Gene Cloning and Expression in Escherichia coli"Nucleic Acids Res. Suppl.. 2. 227-228 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T.Komatsu: "Thermostable Synthetic Hemoprotein"Chem. Lett.. 32. 108-109 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ihsanawati: "Crystallization and Preliminary X-ray Studies of Xylanase 10B from Thermotoga maritima"Acta Cryst.. D59. 1659-1661 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] M.Inami: "Directed Evolution of Xylanase J form Alkaliphilic Bacillus sp. Strain 41M-1"Nucleic Acids Res. Suppl.. 3. 315-316 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] S.Nakamura: "Structure and Function of a Multidomain Alkaline Xylanase from Alkaliphilic Bacillus sp. Strain 41M-1"Catal. Survey Asia. 7. 157-164 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T.Fukazawa: "Chitosan 7B-degrading Enzyme from Alkaliphilic Bacillus sp. Strain J813""Chitin and Chitosan, Chitin and Chitosan in Life Science" (Ed. by T. Uragami et al.), Kodansha, Tokyo. 428-429 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T.Komatsu et al.: "Thermostable Synthetic Hemoprotein"Chem.Lett.. 32. 108-109 (2003)
• [Publications] S.Nakamura: "Structure and Function of a Multidomain Alkaline Xylanase from Alkaliphilic Bacillus sp.Strain 41M-1"Catal.Survey Asia. 7. 157-164 (2003)
• [Publications] Ihsanawati et al.: "Crystallization and Preliminary X-ray Studies of Xylanase 10B from Thermotoga maritima"Acta Cryst.. D59. 1659-1661 (2003)
• [Publications] 中村 聡: "バイオマテリアルへの期待"化学装置. 45. 51-52 (2003)
• [Publications] 中村 聡: "極限環境微生物とその利用:極限酵素の分子手術"BIOベンチャー. 3. 92-94 (2003)
• [Publications] 中村 聡: "アルカリキシラナーゼの分子手術"BIO INDUSTRY. 20. 5-16 (2003)
• [Publications] 中村 聡: "環境修復と有用物質生産-環境問題へのバイオテクノロジーの利用-"シーエムシー. 26-32 (2003)
• [Publications] R.Yatsunami et al.: "A Novel Chitosanase from Bacillus sp. Strain K17 : Gene Cloning and Expression in Escherichia coli"Nucleic Acids Res. Suppl.. 2. 227-228 (2002)
• [Publications] T.Matsuo et al.: "Cloning and Expression of Haloarcula japonica Ferredoxin Gene, and Characterization of Recombinant Proteins"Porphyrins. 11. 108-108 (2002)
• [Publications] T.Komatsu et al.: "Thermostable Synthetic Hemoprotein"Chem.Lett.. 32. 108-109 (2003)
• [Publications] K.Endo et al.: "Molecular Cloning of a Chitinase Gene from Newly Isolated Alkaliphilic Nocardiopsis sp. Strain F96"Extremophiles. (In press). (2003)
• [Publications] 中村 聡: "グリーンバイオケミストリー-ポストゲノム時代を迎え,対話を始めた化学と社会-"現代化学. 2002年4月号. 14-19 (2002)
• [Publications] 中村 聡: "パイオマテリアルへの期待"化学装置. (印刷中). (2003)
• [Publications] 大倉一郎ほか: "新版 生物工学基礎"講談社サイエンティフィク. 150 (2002)
• [Publications] T.Matsuo et al.: "Cloning and Expression of the Ferredoxin Gene from Extremely Halophilic Archaeon Haloarcula japonica Strain TR-1"BioMetals. 14. 135-142 (2001)
• [Publications] 鈴木麻美絵ほか: "大腸菌O157ベロ毒素遺伝子検出系の構築"生理学技術研究会報告(生理学研究所技術課・編). 23. 116-117 (2001)
• [Publications] R.Yatsunami et al.: "Gene clusters encoding ATP sythase of Haloarcula japonica strain TR-1"Nucleic Acids Res.Suppl.. 1. 51-52 (2001)
• [Publications] 中村 聡: "極限環境微生物と極限酵素"BIO INDUSTRY. 18. 14-20 (2001)
• [Publications] 八波利恵ほか: "生体内分子の自己修復"科学と教育. 49. 122-125 (2001)
• [Publications] T.Fukazawa et al.: "Chitin and Chitosan, Chitin and Chitosan in Life Science (ed.by T.Uragami, K.Kurita, T.Fukamizo)"Kodansha Scientific Ltd.. 600 (2001)