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High Resolution X-ray Study of Cofactor Dependent Enzyme and Biocatalyst Design

Research Project

Project/Area Number 13125207
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionOsaka City University

Principal Investigator

HIROTSU Ken  Osaka City University, Graduate School of Science, Osaka City University, Professor, 大学院・理学研究科, 教授 (10047269)

Co-Investigator(Kenkyū-buntansha) MORIMOTO Yukio  Kyoto University, Research Reactor Institute, Professor, 原子炉実験所, 教授 (80200450)
MIYAHARA Ikuko  Osaka City University, Graduate School of Science, Lecturer, 大学院・理学研究科, 講師 (40271176)
柴田 直樹  姫路工業大学, 大学院・理学研究科, 助手 (30295753)
安岡 則武  姫路工業大学, 理学部, 教授 (40029054)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥38,400,000 (Direct Cost: ¥38,400,000)
Fiscal Year 2003: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2002: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2001: ¥23,000,000 (Direct Cost: ¥23,000,000)
Keywordscomposite blocatalyst / glutamine amidotransferase / quinoprotein / pyridoxal phosphate / flavin / X-ray Crystallography / コンポジット触媒 / PLP酵素 / トレオニン合成酵素 / グルタミンアミノ基転移酵素 / CTP合成酵素 / 基質認識 / 結晶構造 / X線結晶解析 / アルギニノコハク酸合成酵素 / グルタミンアミドトランスフェラーゼ / 高度好熱菌 / 分子内チャネル / フラビン依存型酵素 / アミノ基転移酵素 / キノプロテイン / フラビン依存型酸化酵素 / ビルトイン補酵素
Research Abstract

High-resolution Structures of natural composite catalysts such as glutamine amidotransferase, and allosteric CTP synthetase, PLP-dependent enzymes, and FAD-dependent enzymes have been determined by X-ray crystallographic methods. The substrate recognition, induced fit, and reaction mechanism have been analyzed to understand the molecular design which enables these enzymes to act as biocatalysts.
1. Three-dimensional structures of natural biocatalysts and active-site designs. (1)Imidazole glycerol phosphate synthetase. The ammonia transfer mechanism through a molecular channel linking glutaminase and synthase sites has been proposed. (2)CTP (cytidine 5'-triphosphate) synthetase. The mechanism for ammonia tunneling, allosteric GTP activation, and enzyme activation induced by ATP and UTP-dependent conformational change has been examined. (3)Argininosuccinate synthetase. The substrate recognition mechanism and pseudo time-resolved X-ray analysis has been performed in order to clarify the st … More ereochemistry of the reaction. (4) Quinohemoprotein aminedehydrogenase. A novel quinine cofactor and highly unusual thioether cross-bridges have been identified.
2. Three-dimensional structures of PLP-dependent enzymes. (1)Histidinol-phosphate aminotransferase. The active-site design for double substrate recognition has been analyzed. (2)Branched-chain amino acid aminotransferase. The double substrate recognition and reaction mechanism have been elucidated. (3)Threonine synthase. The reaction catalyzed is expected to proceed through all the intermediates utilized by PLP-dependent enzymes. Stereochemistry of the reaction has been proposed. (4)Glutamine/kunerenine aminotransferase. In mammals, the enzyme is involved in CNS events. The substrate recognition and induced fit phenomena have been examined.
3. Three dimensional structures of FAD-dependent enzymes. (1)Medium-chain acyl-CoA oxidase. On the basis of structural comparison with the homologue (acyl-coA dehydrogenase), the molecular design of the oxidase has been analyzed. (2)Medium-chain acyl-CoA dehydrogenase as the transition state analogue. The transition state of the reaction has been examined based on the X-ray structure and MO calculation of the complex. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] H.Mizuguchi, H.Hayashi, K.Okada, I.Miyahara, K.Hirotsu, H.Kagamiyama: "Strain is More Important than Electrostatic Interaction in Controlling the pKa of the Catalytic Group in Aspartate Aminotransferase."Biochemistry. 40(2). 353-360 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Haruyama, T.Nakai, I.Miyahara, K.Hirotsu, H.Mizuguchi, H.Hayashi, et al.: "Structures of Escherichia coli Histidinol-Phosphate Aminotransferase and Its Complexes with Histidinol-Phosphate and N-(5'-Phosphopyridoxyl)- L-Glutamate : Double Substrate Recognition of the Enzyme"Biochemistry. 40(15). 4633-4644 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Okada, K.Hirotsu, H.Hayashi, H.Kagamiyama: "Structures of Escherichia coli Branched-Chain Amino Acid Aminotransferase and Its Complexes with 4-Methylvalerate and 2-Methylleucine : Induced Fit and Substrate Recognition of the Enzyme"Biochemistry. 40(25). 7453-7463 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Tada, C-T.Lu, Y.Nakamura, K.Wada, I.Miyahara, K.Hirotsu, Y.Katsuya, M.Sawada, et al.: "Crystallization and preliminary X-ray analysis of a novel pectolytic enzyme polymethoxy-galactturonase AX1 from Trichosporon penicillatum"Acta Crystallogr D Biol Crystallogr. D57(3). 457-458 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ura, T.Nakai, S.Kawaguchi, I.Miyahara, K.Hirotsu, S.Kuramitsu: "Substrate recognition mechanism of thermophilic dual-substrate enzyme"J.Biochem.. 130(1). 89-98 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Satoh, J-H.Kim, I.Miyahara, B.Devreese, I.Vandenberghe, A.Hacisalihoglu, et al.: "Crystal Structure of Quinohemoprotein Amine Dehydrogenase from Pseudomonas putida"J.Biol.Chem.. 277(4). 2830-2834 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, Y.Nakajima, K.Hirotsu: "Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action"J.Biol.Chem.. 277(18). 15890-15896 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Omi, H.Mizuguchi, M.Goto, I.Miyahara, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Structure of Imidazole Glycerol Phosphate Synthase from Thermus thermophilus HB8 : Open-Closed Conformational Change and Ammonia Tunneling."J Biochem.. 132(5). 759-765 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 佐藤敦子, 広津 建: "最近の研究から「ビルトイン補酵素を持つアミン脱水素酵素の構造研究 - 新規キノン補酵素と異常な分子内架橋-」"日本結晶学会誌. 44. 176-181 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, Y.Agari, R.Omi, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of N-acetyl-gamma-glutamyl-phosphate reductase from Thermus thermophilus HB8"Acta Crystallogr.D Biol.Crystallogr.. 59(2). 356-358 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, R.Omi, J.Hoseki, N.Nakagawa, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8."Acta Crystallogr D Biol Crystallogr. 59(3). 551-553 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Satoh, O.Adachi, K.Tanizawa, K.Hirotsu: "The active site structure of quinohemoprotein amine dehydrogenase inhibited by p-nitrophenyl-hydrazine"Biochimica et Biophysica Acta. 1647(1-2). 272-277 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto I.Miyahara H.Hayashi H.Kagamiyama, K.Hirotsu: "Crystal structures of branched-chain amino Acid aminotransferase complexed with glutamate and glutarate : true reaction intermediate and double substrate recognition of the enzyme."Biochemistry. 42(13). 3725-3733 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Gogo, R.Omi, I.Miyahara, M.Sugahara, K.Hirotsu: "Structures of Argininosuccinate Synthetase in Enzyme-ATP-Substrates and Enzyme-AMP-Product Forms : Stereochemistry of the Catalytic Reaction"J.Biol.Chem.. 278(25). 22964-22971 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Omi, M.Goto, I.Miyahara, H.Mizuguchi, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Crystal Structures of Threonine Synthase from Thermus thermophilus HB8 ; Conformational change, substrate recognition, and mechanism"J Biol Chem.. 278(46). 46035-46045 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 森本幸生: "蛋白質結晶学における二次元検出器の利用"日本結晶学会誌. 45. 289-294 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Omi, M.Goto, N.Nakagawa, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of histidinol phosphate phosphatase"Acta Crystallogr.D Biol.Crystallogr.. 60(3). 574-576 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, R.Omi, I.Miyahara A.Hosono, H.Mizuguchi, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Crystal structures of glutamine : phenylpyruvate aminotransferase from Thermus thermophilus HB8: induced fit and substrate recognition"J.Biol.Chem.. 279(16). 16518-16525 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, R.Omi, N.Nakagawa, I.Miyahara, K.Hirotsu: "Crystal Structures of CTP Synthetase reveal ATP, UTP, and Glutamine Binding Sites"Structure. 12(In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Sato, N.Shibata, Y.Morimoto, Y.Takayama, K.Ozawa, H.Akutsu, Y.Higuchi, N.Yasuoka: "X-ray induced reduction of the crystal of high-molecular weight cytochrome c revealed by microspectrophotometry"J.Synchrotron Rad.. 11(pt1). 113-116 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 森本幸生他: "中性子科学への招待_中性子入門_"日本結晶学会誌. 46. 120-126 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, H.Tamaoki: "Agency for Scientific Publ.Berlin (Eds.Chapman, S., Perham, R., Scrutton, N.)"Flavins and Flavoproteins. 6 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Mizuguchi, H.Hayashi, K.Okada, I.Miyahara, K.Hirotsu, H.Kagamiyama: "Strain is more important than electrostatic interaction in controlling the pKa of the catalytic group in aspartate aminotransferase."Biochemistry. 40. 353-360 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Haruyama, T.Nakai, I.Miyahara, K.Hirotsu, H.Mizuguchi, H.Hayashi, H.Kagamiyama: "Structures of Escherichia coli histidinol-phosphate aminotransferase and its complexes with histidinol-phosphate and N-(5'-phosphopyridoxyl)-L-glutamate : Double substrate recognition of the enzyme."Biochemistry. 40. 4633-4644 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Okada, K.Hirotsu, H.Hayashi, H.Kagamiyama: "Structures of Escherichia coli branched-chain amino acid aminotransferase and its complexes with 4-methylvalerate and 2-methylleucine : Induced fit and substrate recognition of the enzyme."Biochemistry. 40. 7453-7463 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Tada, C-T.Lu, Y.Nakamura, K.Wada, I.Miyahara, K.Hirotsu, Y.Katsuya, M.Sawada, M.Takao, T.Sakai, K.Nishimura: "Crystallization and preliminary X-ray analysis of novel pectolytic enzyme polymethoxy-galactturonase AX1 from Trichosporon penicillatum."Acta Cryst.. D57. 457-458 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ura, T.Nakai, S.Kawaguchi, I.Miyahara, K.Hirotsu, S.Kuramitsu: "Substrate recognition mechanism of thermophilic dual-substrate enzyme."J. Biochem.. 130. 89-98 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Satoh, J-H.Kim, I.Miyahara, B.Devreese, I.Vandenberghe, A.Hacisalihoglu, T.Okajima, S.Kuroda, O.Adachi, J.A.Duine, J.Van Beeumen, K.Tanizawa, K.Hirotsu: "Crystal structure of quinohemoprotein amine dehydrogenase from Pseudomonas putida"J. Biol. Chem.. 277. 2830-2834 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, Y.Nakajima, K.Hirotsu: "Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action."J. Biol. Chem.. 277. 15890-15896 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Omi, H.Mizuguchi, M.Goto, I.Miyahara, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Structure of imidazole glycerol phosphate synthase from Thermus thermophilus HB8 : Open-closed conformational change and ammonia tunneling."J. Biochem.. 132. 759-765 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, Y.Agari, R.Omi, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of N-acetyl-gamma-glutamyl-phosphate reductase from Thermus thermophilus HB8."Acta Crystallogr. D Biol. Crystallogr.. 59. 356-358 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, R.Omi, J.Hoseki, N.Nakagawa, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8."Acta Crystallogr D Biol Crystallogr.. 59. 551-553 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Satoh, O.Adachi, K.Tanizawa, K.Hirotsu: "The active site structure of quinohemoprotein amine dehydrogenase inhibited by p-nitrophenyl-hydrazine."Biochimica et Biophysica Acta. 1647. 272-277 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto I.Miyahara H.Hayashi H.Kagamiyama, K.Hirotsu: "Crystal structures of branched-chain amino acid aminotransferase complexed with glutamate and glutarate : true reaction intermediate and double substrate recognition of the enzyme."Biochemistry. 42. 3725-3733 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Gogo, R.Omi, I.Miyahara, M.Sugahara, K.Hirotsu: "Structures of argininosuccinate synthetase in enzyme-ATP-substrates and enzyme-AMP-product forms : Stereochemistry of the catalytic reaction."J. Biol. Chem.. 278. 22964-22971 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Omi, M.Goto, I.Miyahara, H.Mizuguchi, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Crystal structures of threonine synthase from Thermus thermophilus HB8 : Conformational change, substrate recognition, and mechanism."J. Biol. Chem.. 278. 46035-46045 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Omi, M.Goto, N.Nakagawa, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of histidinol phosphate phosphatase."Acta Crystallogr. D Biol. Crystallogr.. 60. 574-576 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, R.Omi, I.Miyahara A.Hosono, H.Mizuguchi, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Crystal structures of glutamine : phenylpyruvate aminotransferase from Thermus thermophilus HB8 : induced fit and substrate recognition."J. Biol. Chem.. 279. 16518-16525 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, R.Omi, N.Nakagawa, I.Miyahara, K.Hirotsu: "Crystal structures of CTP synthetase reveal ATP, UTP, and glutamine binding sites."Structure, in press. 12. (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Sato, N.Shibata, Y.Morimoto, Y.Takayama, K.Ozawa, H.Akutsu, Y.Higuchi, N.Yasuoka: "X-ray induced reduction of the crystal of high-molecular weight cytochrome c revealed by microspectrophotometry."J. Synchrotron Rad.. 11. 113-116 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Goto, R.Omi, I.Miyahara, A.Hosono, H.Mizuguchi, et al.: "Crystal structures of glutamine : phenylpyruvate aminotransferase from Thermus thermophilus HB8 : Induced fit and substrate recognition"J.Biol.Chem.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Hosono, H.Mizuguchi, H.Hayashi, M.Goto, I.Miyahara, et al.: "Glutamine : phenylpyruvate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8"J.Biochem.. 134(6). 843-851 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Satoh, Y.Nakajima, I.Miyahara, K.Hirotsu, et al.: "Structure of the transition state analog of medium-chain acyl-CoA dehydrogenase."J.Biochem.. 134(6). 297-304 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] R.Omi, M.Goto, I.Miyahara, H.Mizuguchi, H.Hayashi, et al.: "Crystal structures of threonine synthase from Thermus thermophilus HB8: conformational change, substrate recognition, and mechanism"J.Biol.Chem.. 278(46). 46035-46045 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Goto, R.Omi, I.Miyahara, M.Sugahara, K.Hirotsu: "Structures of Argininosuccinate Synthetase in Enzyme-ATP-Substrates and Enzyme-AMP-Product Forms : Stereochemistry of the Catalytic Reaction"J.Biol.Chem.. 278(25). 22964-22971 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Satoh, O.Adachi, K.Tanizawa, K.Hirotsu: "The active site structure of quinohemoprotein amine dehydrogenase inhibited by p-nitrophenyl-hydrazine"Biochimica et Biophysica Acta. 1647(1-2). 272-277 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] C.Setoyama, Y.Nishina, H.Tamaoki, H.Mizutani, I.Miyahara, K.Hirotsu, K.Shiga, R.Miura: "Effects of hydrogen bonds in association with flavin and substrate in flavoenzyme D-amino acid oxidase. The catalytic and structural roles of Gly313 and Thr317"Journal of Biochemistry. 131(1). 59-69 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, H.Tamaoki, R.Miura: "Three-dimensional Structure of the Flavoenzyme Acyl-CoA Oxidase-II from Rat Liver, the Peroxisomal Counterpart of Mitochondrial Acyl-CoA Dehydrogenase"Journal of Biochemistry. 131(3). 365-374 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Goto, Y.Nakajima, K.Hirotsu: "Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action"Journal of Biological Chemistry. 277(18). 15890-15896 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] R.Omi, H.Mizuguchi, M.Goto, I.Miyahara, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Structure of Imidazole Glycerol Phosphate Synthase from Thermus thermophilus HB8 : Open-Closed Conformational Change and Ammonia Tunneling"Journal of Biochemistry. 132(5). 759-765 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Goto, Y.Agari, R.Omi, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of N-acetyl-gamma-glutamyl-phosphate reductase from Thermus thermophilus HB8"Acta Crystallogr. D BioL Crystallogr. 59(2). 356-358 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Goto, R.Omi, J.Hoseki, N.Nakagawa, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8"Acta Crystallogr D Biol Crystallogr. 59(3). 551-553 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, et al.: "Structural Comparison of Acyl-CoA Oxidase-II from Rat liver (Eds. S. Chapman, R. Perham, N. Scrutton, Flavins and Flavoproteins)"Agency for Scientific Publ. Berlin. 6 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Mizuguchi, H.Hayashi, K.Okada, I.Miyahara, K.Hirotsu, et al.: "Strain is More Important than Electrostatic Interaction in Controlling the pKa of the Catalytic Group in Aspartate Aminotransferase"Biochemistry. 40(2). 353-360 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kazuki Haruyama, Tadashi Nakai, Ikuko Miyahara, Ken Hirotsu, et al.: "Structures of Escherichia coli Histidinol-Phosphate Aminotransferase and Its Complexes with Histidinol-Phosphate and N-(5'-Phosphopyridoxyl)-L-Glutamate: Double Substrate Recognition of the Enzyme"Biochemistry. 40(15). 4633-4644 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kengo Okada, Ken Hirotsu, Hideyuki Hayashi, Hiroyuki Kagamiyama: "Structures of Escherichia coli Branched-Chain Amino Acid Aminotransferase and Its Complexes with 4-Methylvalerate and 2-Methylleucine: Induced Fit and Substrate Recognition of the Enzyme"Biochemistry. 40(25). 7453-7463 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Ura, T.Nakai, Si.Kawaguchi, I.Miyahara, K.Hirotsu, S.Kuramitsu: "Substrate recognition mechanism of thermophilic dual-substrate enzyme"The Journal of Biochemistry. 130(1). 89-98 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Satoh, J-K.Kim, I.Miyahara, B.Devreese, I.Vandenberghe, et al.: "Crystal Structure of Quinohemoprotein Amine Dehydrogenase from Pseudomonas putida,Identification of a Novel Quinone Cofactor encaged by Mutiple Thioether Cross-Brudges"J.Biol.Chem.. 277(4). 2830-2834 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, et al.: "Crystallization and preliminary X-ray chsracterization of rat liver acyl-CoA oxidase"The Journal of Biochemistry. D57. 1680-1681 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2018-03-28  

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