| Project/Area Number |
13204043
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyoto University |
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Principal Investigator |
INOUE Gen (2002-2004) Kyoto University, Graduate School of Medicine, Assistant professor, 医学研究科, 講師 (20260606)
中尾 一和 (2004) 京都大学, 医学研究科, 教授 (00172263)
ITOH Hiroshi (2001) Kyoto University, Graduate School of Medicine, Associate Professor, 医学研究科, 助教授 (40252457)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAO Kazuwa Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (00172263)
SAITO Yoshihiko Nara Medical University, Faculty of Medicine, Prefessor, 医学部, 教授 (30250260)
ITOH Hiroshi Kyoto University, Graduate School of Medicine, Associate Professor, 医学研究科, 助教授 (40252457)
INOUE Gen Kyoto University, Graduate School of Medicine, Assistant professor, 医学研究科, 講師 (20260606)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥33,000,000 (Direct Cost: ¥33,000,000)
Fiscal Year 2004: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 2003: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 2002: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2001: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | Hypertension / genome / natriuretic peptide / GC-A / allele frequency / 遺伝子多型 / 高血圧症 / CT繰り返し配列 / ノックアウトマウス / 心室 / 心肥大 / 内皮 / eNOS / 遺伝子 / 本能性高血圧症 / GC-A遺伝子 / 転写活性 / ANP / cGMP / SNP |
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| Research Abstract |
The purpose of this study was to evaluate the significance of the effect of cGMP-related gene on controlling blood pressure in humans. After the informed consents, blood sample was drawn from normotensive (n=180, mean blood pressure 124/74 mmHg) and hypertensive (n=189, mean blood pressure 162/93 mmHg). Extracted genome was subjected to PCR direct sequence method to detect polymorphism in the sequence of guanylyl cyclase-A gene, which is one of the two major types of receptors for natriuretic peptides. As a result, we had detected 5 polymorphisms through the whole span of 22 exsons and 21 introns which length is 16kb. In this study, we investigated one of those polymorphisms, CT repeats in-300 by of 5' franking lesion of this gene. We detected the existence of (CT)6 repeats in addition to already reported (CT) 10, 11, 12 repeats. Moreover, the allele frequency of (CT)6 seems to be significantly different between normotensive and hypertensive. On the other hand, there seems to be no significant difference in the allele frequency of (CT) 10, 11, 12 repeats. We are now confirming our results and on the discussion about its significance on blood pressure control in humans
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