• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

肺腺癌の診断,悪性度診断の客観化を目指した遺伝子診断システムの確立

Research Project

Project/Area Number 13218020
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

深山 正久  東京大学, 大学院・医学系研究科, 教授 (70281293)

Co-Investigator(Kenkyū-buntansha) 仁木 利郎  東京大学, 大学院・医学系研究科, 助教授 (90198424)
後藤 明輝  東京大学, 大学院・医学系研究科, 助手 (90317090)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥40,000,000 (Direct Cost: ¥40,000,000)
Fiscal Year 2004: ¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 2003: ¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 2002: ¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 2001: ¥10,200,000 (Direct Cost: ¥10,200,000)
Keywords肺腺癌 / 悪性度 / 浸潤 / 遺伝子発現 / DNAチップ / マイクロダイセクション / p73 / TSLC1 / バイオインフォマティクス / 浸潤部 / 瘢痕形成 / 低酸素 / 発現不均一性
Research Abstract

肺腺癌に特異的な遺伝子変化,肺腺癌の生物学的挙動に関連した遺伝子変化を同定する目的で,肺腺癌の発現プロファイルの解析を進めた.
1)浸潤関連遺伝子,判別マーカーの同定:レーザーマイクロダイセクション法とDNA chipを用い,肺腺癌辺縁部,中心部癌細胞,ならびに細気管支肺胞上皮癌細胞(BAC)と正常肺における発現プロファイルの比較を行った.正常部で発現が低く,辺縁部癌細胞で発現亢進していたプローブを71個,中心部癌細胞で亢進していたもの105個,辺縁部と中心部で共通して発現亢進が見られたもの81個を抽出した.中心部高発現105プローブから,BACで発現亢進していた25個のプローブを差し引き,重複したプローブ,ESTを差し引いた65個の遺伝子を肺腺癌浸潤関連遺伝子として,解析を進めている.このうち10個は肺非小細胞癌の予後,転移マーカーとして既に報告されていた.47個については,肺以外の臓器の癌で発現亢進,腫瘍形成に関係しているという報告があった.8個が癌での機能が未知の遺伝子であった.さらに肺腺癌とBACの判別マーカーとなる候補遺伝子についても解析を進めている.
2)癌抑制遺伝子候補の臨床病理学的意義:p73ならびにTSLC1の肺腺癌外科切除例に対する発現解析を行った.p73は63例(66%)において癌細胞の核内に陽性所見がみられた.P73陰性所見は65歳以上の症例に多く,発現低下は単変量,多変量解析で有意な予後不良因子であった.一方,TSLC1は,93例中33例にのみ陽性で,TSLC1陰性所見は男性例,低分化癌に多くみられた.TSLC1発現低下は予後不良因子であったが,とりわけ男性例において顕著であった.P73は高齢者,TSLC1は男性腺癌における進展に関与する癌抑制遺伝子であり,不均一な肺腺癌における層別化に有効なバイオマーカーとなることが示唆された.

Report

(4 results)
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All 2005 2004 2003 2002 Other

All Journal Article (6 results) Publications (15 results)

  • [Journal Article] Lung adenocarcinoma associated with familial adenomatous polyposis. Clear cell carcinoma with beta-catenin accumulation accompanied by atypical adenomatous hyperplasia.2005

    • Author(s)
      Goto A et al.
    • Journal Title

      Virchows Arch 446

      Pages: 73-77

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunohistochemical study of Skp2 and Jab1, two key molecules in the degradation of P27, in lung adenocarcinoma.2004

    • Author(s)
      Goto A et al.
    • Journal Title

      Pathology International 54

      Pages: 825-829

    • NAID

      10017450201

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Overexpression of cdk4/cyclin D1, a possible mediator of apoptosis and an indicator of prognosis in human primary lung carcinoma.2004

    • Author(s)
      Dobashi Y et al.
    • Journal Title

      International journal of Cancer 110

      Pages: 532-541

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Lung adenocarcinoma associated with atypical adenomatous hyperplasia. A clinicopathological study with special reference to smoking and cancer multiplicity.2003

    • Author(s)
      Kitagawa H, et al.
    • Journal Title

      Pathology International 53

      Pages: 613-619

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Overexpression of cadherins suppresses pulmonary metastasis of osteosarcoma in vivo2003

    • Author(s)
      Kashima T, et al.
    • Journal Title

      International Journal of Cancer 147

      Pages: 147-154

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Frequent co-localization of cox-2 and laminin-5 g2 chain at the invasive front of early-stage lung adenocarcinoma.2002

    • Author(s)
      Niki T, et al.
    • Journal Title

      American Journal of Pathology 160

      Pages: 1129-1141

    • Related Report
      2004 Annual Research Report
  • [Publications] Kitagawa H, et al.: "Lung adenocarcinoma associated with atypical adenomatous hyperplasia. A clinicopathological study with special reference to smoking and cancer multiplicity."Pathology International. 53. 823-827 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zhang SC, et al.: "Intratumor microvessel density in biopsy specimens predicts local response of hypopharyngeal cancer to radiotherapy."Japanese Journal of Clinical Oncology.. 33. 613-619 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kashima T, et al.: "Overexpression of cadherins suppresses pulmonary metastasis of osteosarcoma in vivo"International Journal of Cancer. 147. 147-154 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Niki T, et al.: "Frequent co-localization of cox-2 and laminin-5 g2 chain at the invasive front of early-stage lung adenocarcinoma."American Journal of Pathology. 160. 1129-1141 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kashima T et al.: "Overexpression of cadherins suppresses pulmonary metastasis of osteosarcoma in vivo"Int J Cancer. 104. 147-154 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hippo Y et al.: "Global gene expression analysis of gastric cancer by oligonucleotide microarrays"Cancer Res. 62. 233-240 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Niki T et al.: "Frequent co-localization of cox-2 and laminin-5 g2 chain at the invasive front of early-stage lung adenocarcinoma"Am.J.Pathol.. 160. 1129-1141 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chong JM et al.: "Interleukin-1beta expression in human gastric carcinoma with Epstein-Barr virus infection"J Virol.. 76. 6825-6831 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hironaka M et al.: "Pulmonary gangliocytic paraganglioma : case report and comparative immunohistochemical study of related neuroendocrine neoplasms"Am.J.Surg.Pathol.. 25. 688-693 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hironaka M et al.: "Pulmonary gangliocytic paraganglioma : case report and comparative immunohistochemical study of related NE neoplasms"Am J Sung Pathol. 25. 688-693 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanaka A et al.: "Purification of activins from androgen-independent Shionogi carcinoma cells demonstrates enhanced expression of activin beta B-subunit・・・・"J Cell Physiol. 188. 194-200 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kobayashi M et al.: "Isolation of an androgen-inducible novel lipocalin gene, Arg1,from androgen-dependent mouse mammary Shionogi carcinoma cells"J Steroid Biochem Mol Biol. 77. 109-115 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hippo Y et al.: "Global gene expression analysis of gastric cancer by oligonucleotide microarrays"Cancer Res. 62. 233-240 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masaki T et al.: "Possible contribution of CD44 variant 6 and nuclear β-catenin expression to the formation of budding tumor Cells・・・"Cancer. 92. 2539-2546 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masaki T et al.: "Matrilysin (MMP-7)as a significant determinant of malignant potential of early invasive colorectal carcinomas"Br J Cancer. 84. 1317-1321 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi