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STUDY ON THE COLD-RESPONSIVE PATHWAYS IN POLYPLOID WHEAT

Research Project

Project/Area Number 13306002
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKobe University

Principal Investigator

NAKAMURA Chinaru  Kobe Univ, Biological and Environmental Science, Professor, 農学部, 教授 (10144601)

Co-Investigator(Kenkyū-buntansha) TAKUMI Shigeo  Kobe Univ, Biological and Environmental Science, Assistant Professor, 農学部, 助手 (50249166)
MORI Naoki  Kobe Univ, Biological and Environmental Science, Associate Professor, 農学部, 助教授 (60230075)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥30,160,000 (Direct Cost: ¥23,200,000、Indirect Cost: ¥6,960,000)
Fiscal Year 2003: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2002: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2001: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Keywordscold acclimation / freezing tolerance / cold-responsive genes / chloroplast / mitochondria / common wheat / ABA-dependent / independent pathways / ABA非依存性経路 / Cor-Ltr / Dhn / Lea / Rab遺伝子群 / Cor
Research Abstract

Cold/freezing tolerance or winter hardiness is an important trait for over-wintering plants. Plants grown in the temperate regions have ability to respond and adapt to low temperature in order to survive severe winter. This adaptive ability manifests itself through the process known as cold hardening or acclimation, which involves significant alternations in gene expression profiles associated with numerous biochemical and physiological changes.
Our aim of this research was to understand the cold-responsive pathways in wheat. We cloned a number of cold-responsive (Cor/Lea) genes and their putative transcription factors, and compared their expression profiles with the developmental time-course of freezing tolerance during cold acclimation of two wheat cultivars with contrasting levels of freezing tolerance. Our major findings can be summarized as follows.
(1) The developmental time course of freezing tolerance shows good correlation with the expression profiles of Cor/Lea genes and a transcription factor Cbf/Dreb1 during cold acclimation. (2) Expression of Cbf/Dreb1 is rapid (within 15 min after exposure to low temperature at 4℃), preceding that of the down-stream Cor/Lea genes. (3) There are two apparent peaks of expression of Cbf/Dreb1 and Cor/Lea genes, i.e., an early response peak at 1-4 hours and a long-term response peak at 2-3 weeks after cold acclimation. (4) Elevated levels of expression of Cbf/Dreb1 and Cor/Lea genes are associated with the recessive state of vernalization responsive genes vrn1, thus likely with the dominant state of frost/freezing resistance genes Fr1. (5) A major cold-responsive pathway found in Arabidopsis is conserved in the wheat genome. (6) The wheat genome possibly possesses a novel cold-responsive pathway(s).

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Ohno R., et al.: "Expression of a cold-responsive Lt-Cor gene and development of freezing tolerance during cold acclimation in wheat(Triticum aestivum L.)"J.of Experimental Botany. 52. 2367-2374 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takumi S., et al.: "Characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat"Genes & Genetic Systems. 77. 81-88 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno R., et al.: "Kinetics of transcript and protein accumulation of a low-molecular-weight wheat LEA D-11 dehydrin in response to low temperature"J.of Plant Physiology. 160. 193-200 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takumi S., et al.: "Cold-specific and light-stimulated expression of a wheat (Triticum aestivum L.) Cor gene Wcor15 encoding a chloroplast-targeted protein"J.of Experimental Botany. 54. 2265-2274 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takumi S., et al.: "Cultivar differences in cold acclimation/freezing tolerance and Cor gene expression in common wheat"Proc.10th Int.Wheat Genet.Symp.,Paestam, Italy. 3. 1269-1271 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, F., et al.: "Effects of Vm-Frintervals on the homoeologous group 5 chromosomes on cold responsive gene expression in common wheat"Proc.10th Int.Wheat Genet.Symp.,Paestam, Italy. 3. 1174-1176 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi F., et al.: "Comparative study of the expression profiles of a Col/Lea gene family in two wheat cultivars with contrasting levels of freezing tolerance"Physiologia Plantarum. 120. 585-594 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Naydenov, N., et al.: "A possible energy-conserving function of alternative oxidase during seed germination"Recent Research Developments in Biochemistry. 5. 145-156 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno, R.et al.: "Expression of cold-responsive Lt-Cor gene and development of freezing tolerance during cold acclimation in wheat (Triticum aeslivum L.)"J.ExBot.. 52. 2367-2374 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takumi, S.et al.: "Characterization of two non-homologous nuclear genes encoding mitochondrial alternative oxidase in common wheat"Genes & Genet.Syst.. 77. 81-88 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno, R., et al.: "Kinetics of transcript and protein accumulation of a low-molecular-weight wheat LEAD-11 dehydrin in response to low temperature"J.Plant Physiol.. 160. 193-200 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takumi, S.et al.: "Cold-specific and light-stimulated expression of a wheat (Triticum aestivum L.) Cor gene Wcor15 encoding a chloroplast-targeted protein"J.ExBot.. 2265-2274 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takumi, S.et al.: "Cultivar differences on cold cold acclimation/freezing tolerance and Cor gene expression in common wheat"Proc.10^<th> Int.Wheat Genet.Sym. 3. 1269-1271 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, F.et al.: "Effects of Vrn-Fr intervals on the homoeologous group 5 chromosomes on cold responsive gene expression in common wheat"Proc.10^<th> Int.Wheat Genet.Sym. 3. 1174-1176 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayshi, F.et al.: "Comparative study of the expression profiles of a Cor/Lea gene family in two wheat cultivars with contrasting levels of freezing tolerance"Physiol.Plant.. 120. 585-594 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Naydenov, N.et al.: "A possible energy-conserving function of alternative oxidase during seed germination"Recent.Res.Devel.in Biochem. 5. 145-156 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno R.et al.: "Expression of a cold-responsive Lt-Cor gene and development of freezing tolerance during cold acclimation in wheat (Triticum aestivum L.)"J.of Experimental Botany. 52. 2367-2374 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takumi S. et al.: "Characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat"Genes & Genetic Systems. 77. 81-88 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohno R.et al.: "Kinetics of transcript and protein accumulation of a low-molecular-weight wheat LEA D-11 dehydrin in response to low temperature"J.of Plant Physiology. 160. 193-200 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takumi S. et al.: "Cold-specific and light -stimulated expression of a wheat (Triticum aestivum L.) Corgene Wcor15 encoding a chloroplast-targeted protein"J.of Experimental Botany. 54. 2265-2274 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kobayashi F. et al.: "Comparative study of the expression profiles of a Col/Lea gene family in two wheat cultivars with contrasting levels of freezing tolerance"Physiologia Plantarum. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Naydenov, N. et: "A possible energy-conserving function of alternative oxidase during seed germination"Recent Research Developments in Biochemistry. 5(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohno R et al.: "Expression of a cold-responsive Lt-Cor gene and development of freezing tolerance during cold acclimation in wheat (Triticum aestivum L.)"J Experimental Botany. 52. 2367-2374 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takumi S et al.: "Characterization of two non-homeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat"Genes & Genetic Systems. 77. 81-88 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohno R et al.: "Kinetics of transcript and protein accumulation of a low-molecular weight wheat LEA D-11 dehydrin in response to low temeprature"J Plant Physiology. 160. 19 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takumi S et al.: "Cold specific expression of a wheat (Triticum aestivum L.) Cor gene Wcor15 encoding a chloroplast targeted protein"J Experimental Botany. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohno R.et al.: "Expression of a cold-responsive Lt-Cor gene and development of freezing tolerance during cold acclimation in wheat(Triticum aestivum L.)"J. Experimental Botany. 52. 2367-2374 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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