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The host-switching mechanism of phytoplasmas between plants and animals towards the elucidation of the host determination system

Research Project

Project/Area Number 13306004
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionThe University of Tokyo

Principal Investigator

NAMBA Shigetou  The University of Tokyo, Graduate School of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (50189221)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Yukio  The University of Tokyo, Graduate School of Frontier Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (60125987)
SATO Mamoru  National Institute of Agrobiological Sciences, Research Leader, 主席研究管理官
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥37,700,000 (Direct Cost: ¥29,000,000、Indirect Cost: ¥8,700,000)
Fiscal Year 2003: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2002: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2001: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Keywordssmall circular DNA / complete genomic analysis / host factor for insect transmissibility / ATP synthase / leafhopper / host switching / ヨコバイ / ミュータント / 昆虫非伝搬性変異株 / 特異抗体 / 遺伝子発現
Research Abstract

In this year, we determined the complete genomic sequence of the plant-pathogenic bacterium Candidatus Phytoplasma asteris. Comparison of the genome of P.asteris with those of other parasitic and symbiotic bacteria reveals features that are unique to this intracellular parasite, which has adapted to a nutrient-rich environment. The genome of P.asteris encodes genes for basic functions, such as DNA replication, transcription, and translation. In contrast, genes responsible for amino acid and fatty acid biosynthesis, the TCA cycle, and oxidative phosphorylation are absent. This may be because phytoplasmas can depend on their host cells for supplies of many biological substances, as is the case with mycoplasmas. Surprisingly, the P.asteris genome encodes proteins for even fewer metabolic functions than do those of mycoplasmas. For example, the genes for the pentose phosphate cycle and ATP synthase have not been found. R.prowazekii has also lost the genes for the pentose phosphate cycle ; however, this organism has retained genes for the TCA cycle, electron transfer system, and ATP synthase. The complete P.asteris genome suggests that the set of known metabolic pathways of phytoplasmas is even smaller than that of mycoplasmas, representing the most minimal genome among living organisms. Instead, P.asteris possesses genes for transporter proteins involved in the uptake of substances from the surrounding host cell cytoplasm. This may be the result of reductive evolution, as a consequence of life as an intracellular parasite in a nutrient-rich environment Phytoplasmas are unique bacteria that contain a new minimal gene set, and this finding should help to elucidate the host determination mechanisms of phytoplasmas.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All 2004 2003 2001 Other

All Journal Article (9 results) Patent(Industrial Property Rights) (1 results) Publications (16 results)

  • [Journal Article] Secretion of immunodominant membrane protein from onion yellows phytoplasmathrough the Sec protein-translocation system in Escherichia coil.2004

    • Author(s)
      Kakizawa, S., Oshima, K., Nishigawa, H., Jung, H-Y., Wei, W., Suzuki, S., Tanaka, M., Miyata, S., Ugaki, M., Namba, S.
    • Journal Title

      Microbiology 150

      Pages: 135-142

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] First complete nucleotide sequence and heterologous gene organization of the two rRNA operons in the phytoplasma genome.2003

    • Author(s)
      Jung, H-Y., Miyata, S., Oshima, K., Kakizawa, S., Nishigawa, H., Kuboyama, T., Ugaki, M., Hibi, T., Namba,S.
    • Journal Title

      DNA and Cell Biology 22

      Pages: 209-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Two different thymidylate kinase gene homologues, including one that has catalytic activity, are encoded in the onion yellows phytoplasma genome.2003

    • Author(s)
      Miyata, S., Oshima, K., Kakizawa, S., Nishigawa, H., Jung, H-Y., Kuboyama, T., Ugaki, M., Namba, S.
    • Journal Title

      Microbiology 149

      Pages: 2243-2250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] "Condidatus Phytoplasma ziziphi", a novel phytoplasma taxon associated with jujube witches' broom disease.2003

    • Author(s)
      Jung, H-Y., Sawayanagi, T., Kakizawa, S., Nishigawa, H., Wei, W., Oshima, K., Miyata, S., Ugaki, M., Hibi, T., Namba, S.
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 53

      Pages: 1037-1041

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Reductive evolution suggested from the complete genome sequence of a plant-pathogenic phytoplasma.2003

    • Author(s)
      Oshima, K., Kakizawa, S., Nishigawa, H., Jung, H-Y., Wei, W., Suzuki, S., Arashida, R., Nakata, D., Miyata, S., Ugaki, M., Namba, S.
    • Journal Title

      Nature Genetics 36

      Pages: 27-29

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] First complete nucleotide sequence and heterologous gene organization of the two rRNA operons in the phytoplasma genome.2003

    • Author(s)
      Jung, H-Y., Miyata, S., Oshima, K., Kakizawa, S., Nishigawa, H., Kuboyama, T., Ugaki, M., Hibi, T., Namba, S.
    • Journal Title

      DNA and Cell Biology 22

      Pages: 209-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] "Candidatus Phytoplasma ziziphi", a novel phytoplasma taxon associated with jujube witches' broom disease.2003

    • Author(s)
      Jung, H-Y., Sawayanagi, T., Kakizawa, S., Nishigawa, H., Wei, W., Oshima, K., Miyata, S., Ugaki, M., Hibi, T., Namba, S.
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 53

      Pages: 1037-1041

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] "Candidatus Phytoplasma oryzae", a novel phytoplasma taxon associated with rice yellow dwarf disease.2003

    • Author(s)
      Jung, H-Y., Sawayanagi, T., Wongkaew, P., Kakizawa, S., Nishigawa, H., Wei, W., Oshima, K., Miyata, S., Ugaki, M., Hibi, T., Namba, S.
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 53

      Pages: 1925-1929

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Secretion of immunodominant membrane protein from onion yellows phytoplasmathrough the Sec protein-translocation system in Escherichia coli.

    • Author(s)
      Kakizawa, S., Oshima K., Nishigawa, H., Jung, H-Y., Wei, W., Suzuki, S., Tanaka, M., Miyata, S., Ugaki, M., Namba, S.
    • Journal Title

      Microbiology 150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Patent(Industrial Property Rights)] ファイトプラズマの検出方法2001

    • Inventor(s)
      難波成任
    • Industrial Property Rights Holder
      難波成任
    • Filing Date
      2001-11-12
    • Acquisition Date
      2004-01-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jung, H-Y., Miyata, S., Oshima, K., Kakizawa, S., Nishigawa, H., Kuboyama, T., Ugaki, M., Hibi, T., Namba, S.: "First complete nucleotide sequence and heterologous gene organization of the two rRNA operons in the phytoplasma genome."DNA and Cell Biology. 22. 209-215 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyata, S., Oshima, K., Kakizawa, S., Nishigawa, H., Jung, H-Y., Kuboyama, T., Ugaki, M., Namba, S.: "Two different thymidylate kinase gene homologues, including one that has catalytic activity, are encoded in the onion yellows phytoplasma genome."Microbiology. 149. 2243-2250 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Jung, H-Y., Sawayanagi, T., Kakizawa, S., Nishigawa, H., Wei, W., Oshima, K., Miyata, S., Ugaki, M., Hibi, T., Namba, S.: ""Candidatus Phytoplasma ziziphi", a novel phytoplasma taxon associated with jujube witches' broom disease."International Journal of Systematic and Evolutionary Microbiology. 53. 1037-1041 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Jung, H-Y., Sawayanagi, T., Wongkaew, P., Kakizawa, S., Nishigawa, H., Wei, W., Oshima, K., Miyata, S., Ugaki, M., Hibi, T., Namba, S.: ""Candidatus Phytoplasma oryzae", a novel phytoplasma taxon associated with rice yellow dwarf disease."International Journal of Systematic and Evolutionary Microbiology. 53. 1925-1929 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kakizawa, S., Oshima, K., Nishigawa, H., Jung, H-Y., Wei, W., Suzuki, S., Tanaka, M., Miyata, S., Ugaki, M., Namba, S.: "Secretion of immunodominant membrane protein from onion yellows phytoplasma through the Sec protein-translocation system in Escherichia coli."Microbiology. 150. 135-142 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oshima, K., Kakizawa, S., Nishigawa, H., Jung, H-Y., Wei, W., Suzuki, S., Arashida, R., Nakata, D., Miyata, S., Ugaki, M., Namba, S.: "Reductive evolution suggested from the complete genome sequence of a plant-pathogenic phytoplasma."Nature Genetics. 36. 27-29 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishigawa, H., Oshima, K., Kakizawa, S., Jung, H.-Y., Kuboyama, T., Miyata, S., Ugaki, M., Namba, S.: "A plasmid from a non-insect-transmissible line of a phytoplasma lacks two open reading frames that exist in the plasmid from the wild-type line"Gene. 298. 195-201 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishigawa, H., Oshima, K., Kakizawa, S., Jung, H.-Y., Kuboyama, T., Miyata, S., Ugaki, M., Namba, S.: "Evidence of intermolecular recombination between extrachromosomal DNAs in phytoplasma : a trigger for the biological diversity of phytoplasma?"Microbiology. 148. 1389-1396 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oshima K., Miyata, S., Sawayanagi, T., Kakizawa, S., Nishigawa, H., Jung, H.-Y., Furuki, K., Yanazaki, M., Suzuki, S., Wei, W., Kuboyama, T., Ugaki, M., Namba, S.: "Minimal set of metabolic pathways suggested from the genome of onion yellows phytoplasma"J. Gen. Plant Pathol.. 68. 225-236 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyata, S., Furuki, K., Oshima, K., Sawayanagi, T., Nishigawa, H., Kakizawa, S., Jung, H-Y., Ugaki, M., Namba, S.: "Complete nucleotide sequence of the S10-spc operon of phytoplasma : gene organization and genetic code resemble those of Bacillus subtilis"DNA Cell Biol.. 21. 527-534 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Jung, H.-Y., Sawayanagi, T., Kakizawa, S., Nishigawa, H., Miyata, S., Oshima, K., Ugaki, M., Lee J.-T., Hibi, T., Namba, S.: ""Candidatus Phytoplasma castaneae", a novel phytoplasma taxon associated with chestnut witches' broom disease"Int. J. Syst. Evol. Microbiol.. 52. 1543-1549 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishigawa, H., Miyata, S., Oshima, K., Sawayanagi, T., Komoto, A., Kuboyama, T., Matsuda, I., Tsuchizaki, T., Namba, S.: "In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteins"Microbiology. 147. 507-513 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Oshima, K., Kakizawa, S., Nishigawa, H., Kuboyama, T., Miyata, S., Ugaki, M., Namba, S.: "A Plasmid of phytoplasma encodes a unique replication protein having both plasmid- and virus-like domains : Clue to viral ancestry or result of virus/plasmid recombination?"Virology. 285. 270-277 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Oshima, K., Shiomi, T., Kuboyama, T., Sawayanagi, T., Nishigawa, H., Kakizawa, T., Miyata, S., Ugaki, M., Namba, S.: "Isolation and characterization of derivative lines of the onion yellows phytoplasma that do not cause stunting or phloem hyperplasia"Phytopathology. 91. 1024-1029 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kakizawa, S., Oshima, K., Kuboyama, T., Nishigawa, H., Jung, H.-Y., Sawayanagi, T., Tsuchizaki, T., Miyata, S., Ugaki, M., Namba, S.: "Cloning and expression analysis of phytoplasma protein translocation genes"Molecular Plant-Microbe Interaction. 14. 1043-1050 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jung, H.-Y., Sawayanagi, T., Kakizawa, T., Nishigawa, H., Oshima, K., Miyata, S., Kuboyama, T., Ugaki, M., Namba, S.: "Phylogenetic classification of the phytoplasma ; Identification new phytoplasma from disease chestnut tree in Korea"Japanese Jouranl of Mycoplasmology. 28. (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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